Nasp (NM_001081475) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207154
Nasp (Myc-DDK-tagged) - Mouse nuclear autoantigenic sperm protein (histone-binding) (Nasp), transcript variant 1
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nasp
Synonyms 5033430J04Rik; AI131596; AI317140; D4Ertd767e; Epcs32; Nasp-T
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207154 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACAGAGTCTACAGCCGCTGCTGCCATCGCCGCGGAGCTGGTTTCCGCTGACAAAATTGAAGATG
CTCCTGCTCCTTCTACCTCGGCAGATAAAATGGAGAGTCTGGATGTGGATAGTGAAGCTAAGAAACTACT
GGGATTAGGACAGAAACATCTGGTGATGGGTGACATTCCAGCAGCTGTTAATGCATTCCAGGAAGCAGCT
AGTCTTTTAGGTAAGAAGTATGGAGAAACGGCTAATGAGTGTGGAGAAGCCTTCTTTTTTTATGGGAAGT
CACTTCTGGAGTTAGCCAGAATGGAGAATGGAGTGTTGGGAAATGCCTTAGAAGGAGTGCATGTAGAAGA
GGAAGAAGGAGAAAAAACAGAAGATGAATCTTTGGTAGAAAATAATGATAATGTAGATGAAACTGAAGGC
TCAGAGGAAGAGGACAGAGAAAATGACAAGGCTGAAGAAACACCAAATGAATCAGTTCTTGAAAAAAAGT
CTCTTCAAGAAAATGAAGAGGAGGAGATAGGGAATTTGGAACTTGCCTGGGATATGCTGGATTTAGCCAA
GATCATTTTTAAGAGGCAAGAAACAAAAGAAGCCCAGCTTTATGCTGCACAAGCCCATCTTAAACTTGGA
GAAGTTAGTGTTGAATCTGAGAATTATATCCAAGCTGTGGAGGAGTTTCAGGCTTGCCTAAGCCTGCAAG
AACAATATTTGGAAGCTCACGATCGTCTCCTTGCAGAGACTCACTACCAATTAGGTTTGGCCTATGGTTA
CAACTCTCAGTATGATGAAGCAGTAGCACAGTTTGGCAAATCTATCGATGTCATTGAAAAGAGAATGGCT
GTACTCCATGAGCAGATGAAGGAGGCCGAAGGATCGTTTACTGAATATGAGAAAGAAATTGAGGAGCTGA
AAGAACTGCTACCTGAAATTCGAGAGAAGATAGAAGACGCAAAGGAATCTCAGCGGAGTGGGAATGTAGC
CGAACTGGCACTGAAAGCTACTCTGGTGGAGAGCTCTACTTCCGGTTTCACTCCTAGTGGAGCTGGTGCC
TCAGTATCCATGATTGCCAGTAGAAAACCAACAGATGGCGCTTCCTCATCAAATTGTGTGACTGATATTT
CCCATCTTGTCAGAAAGAAGAGGAAACCAGAGGAAGAGAGTCCCCGGAAGGATGATGCAAAGAAAGCTAA
ACAAGAGCCAGAGGTGAATGGAGGCAGTGGGGATGCTGTCTCCAGTGGAAAGGAAGTTTCAGAAAACATG
GAGGCCGAGGCTGAGAATCAGGCTGAGAGCCAAACAGCAGAGGGGACCGTGGAGTCTGCAGCTACAATTA
AAAGCACTGCATGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207154 protein sequence
Red=Cloning site Green=Tags(s)

MATESTAAAAIAAELVSADKIEDAPAPSTSADKMESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAA
SLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNVDETEG
SEEEDRENDKAEETPNESVLEKKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLG
EVSVESENYIQAVEEFQACLSLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFGKSIDVIEKRMA
VLHEQMKEAEGSFTEYEKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPSGAGA
SVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVSSGKEVSENM
EAEAENQAESQTAEGTVESAATIKSTAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081475
ORF Size 1344 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081475.1, NP_001074944.1
RefSeq Size 2118 bp
RefSeq ORF 1347 bp
Locus ID 50927
UniProt ID Q99MD9
Cytogenetics 4 53.24 cM
MW 48.8 kDa
Summary Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and linker H1 histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081475" in other vectors (3)
SKU Description Size Price
MC205656 Nasp (untagged) - Mouse nuclear autoantigenic sperm protein (histone-binding) (Nasp), transcript variant 1, (10ug) 10 ug
$457.00
MR207154L3 Lenti ORF clone of Nasp (Myc-DDK-tagged) - Mouse nuclear autoantigenic sperm protein (histone-binding) (Nasp), transcript variant 1 10 ug
$757.00
MR207154L4 Lenti ORF clone of Nasp (mGFP-tagged) - Mouse nuclear autoantigenic sperm protein (histone-binding) (Nasp), transcript variant 1 10 ug
$757.00

Plasmid Map for MR207154

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Cloning scheme for MR207154

Circular map for MR207154

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