Clu (NM_013492) Mouse Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Clu |
| Synonyms | A; AI893575; ApoJ; C; Cli; D14Ucl; D14Ucla3; Sg; Sgp; Sgp-2; Sgp2; SP-; SP-40; Sugp; Sugp-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013492 |
| ORF Size | 1344 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013492.3 |
| RefSeq Size | 1808 bp |
| RefSeq ORF | 1347 bp |
| Locus ID | 12759 |
| UniProt ID | Q06890 |
| Cytogenetics | 14 34.36 cM |
| MW | 51.7 kDa |
| Summary | The protein encoded by this gene is a secreted chaperone that can, under some stress conditions, also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. The encoded preproprotein undergoes proteolytic processing to generate a disulfide-linked heterodimeric mature protein comprised of alpha and beta subunits. Mice lacking the encoded protein exhibit increased severity of autoimmune myocarditis, faster progression of the acute inflammation to myocardial scarring and decreased brain injury following neonatal hypoxic-ischemic injury. provided by RefSeq, Nov 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013492" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC203543 | Clu (untagged) - Mouse clusterin (Clu), (10ug) | 10 ug |
$686.00
|
|
| MG207148 | Clu (tGFP-tagged) - Mouse clusterin (Clu) | 10 ug |
$886.00
|
|
| MR207148L3 | Lenti ORF clone of Clu (Myc-DDK-tagged) - Mouse clusterin (Clu) | 10 ug |
$986.00
|
|
| MR207148L4 | Lenti ORF clone of Clu (mGFP-tagged) - Mouse clusterin (Clu) | 10 ug |
$986.00
|
|
Citations
Clusterin secreted from astrocyte promotes excitatory synaptic transmission and ameliorates Alzheimer's disease neuropathology
Chen, Swartzlander, Ghosh et al
Mol Neurodegener (2021) 16 (1), 5 DOI: 10.1186/s13024-021-00426-7
Chen, Swartzlander, Ghosh et al
Mol Neurodegener (2021) 16 (1), 5 DOI: 10.1186/s13024-021-00426-7
Cited by 103
AI Summary: OriGene’s mouse Clu cDNA plasmid was used to transfect HEK293 cells, enabling the production of clusterin-conditioned media that was critical for assessing the effects of astrocyte-secreted clusterin on synaptic transmission in this study.
Snippet: Clu CM was collected from cultured HEK293 cells. Briefly, ~ 90% confluent HEK293 cells were transfected with plasmid containing mouse Clu cDNA or empty vector (Origene) using Lipofectamine 3000 (Invitrogen) following the manufacturer's instructions
Brain clusterin protein isoforms and mitochondrial localization
Herring, Moon, Rawal et al
Elife (2019) 8 DOI: 10.7554/eLife.48255
Herring, Moon, Rawal et al
Elife (2019) 8 DOI: 10.7554/eLife.48255
AI Summary: OriGene’s pCMV6-mouse CLU Exon 2–9 (C-terminal Myc-DDK-tagged) plasmid (Cat. #MR207148) was used to generate overexpression constructs for studying clusterin isoforms and their cellular localization in the brain.
Snippet: … GENERATION OF OVEREXPRESSION CONSTRUCTS pCMV6-mouse CLU Exon 2–9 (C-terminal Myc-DDK-tagged) was purchased from OriGene (Cat. #MR207148). The mouse CLU Exon 3–9 was amplified by PCR from cDNA of the mouse CLU Exon 2–9 plasmid using two oligonucleotide primers, mouse CLU Exon 3–9 forward [5′-_GACGGCGATCG;... … GENERATION OF OVEREXPRESSION CONSTRUCTS pCMV6-mouse CLU Exon 2–9 (C-terminal Myc-DDK-tagged) was purchased from OriGene (Cat. #MR207148). The mouse CLU Exon 3–9 was amplified by PCR from cDNA of the mouse CLU Exon 2–9 plasmid using two oligonucleotide primers, mouse CLU Exon 3–9 forward [5′-_GACGGCGATCG
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
