Zic1 (NM_009573) Mouse Tagged ORF Clone
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Zic1 |
| Synonyms | ZIC; ZNF201 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009573 |
| ORF Size | 1341 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009573.4 |
| RefSeq Size | 3350 bp |
| RefSeq ORF | 1344 bp |
| Locus ID | 22771 |
| UniProt ID | P46684 |
| Cytogenetics | 9 48.26 cM |
| MW | 48.3 kDa |
| Summary | Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009573" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC203641 | Zic1 (untagged) - Mouse zinc finger protein of the cerebellum 1 (Zic1), (10ug) | 10 ug |
$457.00
|
|
| MR207138L3 | Lenti ORF clone of Zic1 (Myc-DDK-tagged) - Mouse zinc finger protein of the cerebellum 1 (Zic1) | 10 ug |
$757.00
|
|
| MR207138L4 | Lenti ORF clone of Zic1 (mGFP-tagged) - Mouse zinc finger protein of the cerebellum 1 (Zic1) | 10 ug |
$757.00
|
|
Citations
The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes
Lu, Heyne, Dror et al
Cell Metab. (2018) 27 (6), 1294-1308.e7 DOI: 10.1016/j.cmet.2018.04.013
Lu, Heyne, Dror et al
Cell Metab. (2018) 27 (6), 1294-1308.e7 DOI: 10.1016/j.cmet.2018.04.013
AI Summary: OriGene’s mouse cDNA ORF clones for six different transcription factors were used to over-express these factors in Min6-B1 cells, enabling the study of their roles in β cell identity and dysfunction in the context of diabetes.
Snippet: Transcription Factor Over-Expression Min6-B1 cell line were electroporated using Nucleofector II device (Lonza, Kit T, Program O-017) with pMAXGFP (Lonza) or mouse cDNA ORF clones of the 6 different transcription factors (Origene). 4 days after electroporation, cells were harvested, trypsinized and single cells were sorted in 384-well plates containing 240 nL of primer mix and 1.2 μL of PCR encaps...
Gene regulatory networks in neural cell fate acquisition from genome-wide chromatin association of Geminin and Zic1
Sankar, Yellajoshyula, Zhang et al
Sci Rep (2016) 6, 37412 DOI: 10.1038/srep37412
Sankar, Yellajoshyula, Zhang et al
Sci Rep (2016) 6, 37412 DOI: 10.1038/srep37412
AI Summary: OriGene products contributed to this study by providing full-length murine Geminin (MR202178) and Zic1 (MR207138) cDNAs in expression vectors, which were used to investigate the roles of these transcription factors in neural cell fate acquisition.
Snippet: Full length murine Geminin (MR202178) and Zic1(MR207138) cDNAs in expression vectors were purchased from Origene
Showing 1-2 of 2 papers.
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