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Rtn4rl1 (NM_177708) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207106
Rtn4rl1 (Myc-DDK-tagged) - Mouse reticulon 4 receptor-like 1 (Rtn4rl1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rtn4rl1
Synonyms Ngr3; Ngrh2; Ngrl2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207106 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCGCAAAGGGTGCTGTGTGGAATTGCTGCTGTTGCTGCTCGCTGGAGAGCTACCTCTGGGTGGTG
GTTGTCCTCGAGACTGTGTGTGCTACCCTGCGCCCATGACTGTCAGCTGCCAGGCACACAACTTTGCTGC
CATCCCGGAGGGCATCCCAGAGGACAGTGAGCGCATCTTCCTGCAGAACAATCGCATCACCTTCCTCCAG
CAGGGCCACTTCAGCCCCGCCATGGTCACCCTCTGGATCTACTCCAACAACATCACTTTCATTGCTCCCA
ACACCTTCGAGGGCTTTGTGCATCTGGAGGAGCTAGACCTTGGAGACAACCGACAGCTGCGAACGCTGGC
ACCCGAGACCTTCCAAGGCCTGGTGAAGCTTCACGCCCTCTACCTCTATAAGTGTGGACTGAGCGCCCTG
CCCGCAGGCATCTTTGGTGGCCTGCACAGCCTGCAGTATCTCTACTTGCAGGACAACCATATCGAGTACC
TCCAAGATGACATCTTTGTGGACCTGGTCAATCTCAGTCACTTGTTTCTCCATGGTAACAAGCTATGGAG
CCTGGGCCAAGGCATCTTCCGGGGCCTGGTGAACCTGGACCGGTTGCTGCTGCATGAGAACCAGCTACAG
TGGGTTCACCACAAGGCTTTCCATGACCTCCACAGGCTAACCACCCTCTTTCTCTTCAACAACAGCCTCA
CTGAGCTGCAGGGTGACTGTCTGGCCCCCCTGGTGGCCTTGGAGTTCCTTCGCCTCAATGGGAATGCTTG
GGACTGTGGCTGCCGGGCACGTTCCCTGTGGGAATGGCTGCGAAGGTTCCGTGGCTCTAGCTCTGCTGTC
CCCTGCGCGACCCCCGAGCTGCGGCAAGGCCAGGATCTGAAGCTGCTGAGGGTGGAGGACTTCCGGAACT
GCACAGGACCAGTGTCTCCTCACCAGATCAAGTCTCACACGCTTACCACCTCTGACAGGGCTGCCCGCAA
GGAGCACCATCCGTCCCATGGGGCCTCCAGGGACAAAGGCCACCCACATGGCCATCCGCCTGGCTCCAGG
TCAGGTTACAAGAAGGCAGGCAAGAACTGCACCAGCCACAGGAACCGGAACCAGATCTCTAAGGTGAGCT
CTGGGAAAGAGCTTACCGAACTGCAGGACTATGCCCCCGACTATCAGCACAAGTTCAGCTTTGACATCAT
GCCCACCGCACGACCCAAGAGGAAGGGCAAGTGTGCTCGCAGGACCCCCATCCGTGCCCCCAGTGGGGTG
CAGCAGGCATCCTCAGGCACGGCCCTTGGGGCCCCACTCCTGGCCTGGATACTGGGGCTGGCAGTCACTC
TCCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207106 protein sequence
Red=Cloning site Green=Tags(s)

MLRKGCCVELLLLLLAGELPLGGGCPRDCVCYPAPMTVSCQAHNFAAIPEGIPEDSERIFLQNNRITFLQ
QGHFSPAMVTLWIYSNNITFIAPNTFEGFVHLEELDLGDNRQLRTLAPETFQGLVKLHALYLYKCGLSAL
PAGIFGGLHSLQYLYLQDNHIEYLQDDIFVDLVNLSHLFLHGNKLWSLGQGIFRGLVNLDRLLLHENQLQ
WVHHKAFHDLHRLTTLFLFNNSLTELQGDCLAPLVALEFLRLNGNAWDCGCRARSLWEWLRRFRGSSSAV
PCATPELRQGQDLKLLRVEDFRNCTGPVSPHQIKSHTLTTSDRAARKEHHPSHGASRDKGHPHGHPPGSR
SGYKKAGKNCTSHRNRNQISKVSSGKELTELQDYAPDYQHKFSFDIMPTARPKRKGKCARRTPIRAPSGV
QQASSGTALGAPLLAWILGLAVTLR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177708
ORF Size 1335 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177708.5
RefSeq Size 3308 bp
RefSeq ORF 1338 bp
Locus ID 237847
UniProt ID Q8K0S5
Cytogenetics 11 B5
MW 49.8 kDa
Summary Cell surface receptor that plays a functionally redundant role in postnatal brain development and in regulating axon regeneration in the adult central nervous system (PubMed:22406547, PubMed:27339102). Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum (PubMed:27339102). Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG (PubMed:26335717). Plays a role in inhibiting neurite outgrowth and axon regeneration via its binding to neuronal chondroitin sulfate proteoglycans (PubMed:22406547). Binds heparin (PubMed:22406547). Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:22325200).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177708" in other vectors (4)
SKU Description Size Price
MC203049 Rtn4rl1 (untagged) - Mouse reticulon 4 receptor-like 1 (Rtn4rl1), (10ug) 10 ug
$457.00
MG207106 Rtn4rl1 (tGFP-tagged) - Mouse reticulon 4 receptor-like 1 (Rtn4rl1) 10 ug
$657.00
MR207106L3 Lenti ORF clone of Rtn4rl1 (Myc-DDK-tagged) - Mouse reticulon 4 receptor-like 1 (Rtn4rl1) 10 ug
$757.00
MR207106L4 Lenti ORF clone of Rtn4rl1 (mGFP-tagged) - Mouse reticulon 4 receptor-like 1 (Rtn4rl1) 10 ug
$757.00

Plasmid Map for MR207106

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207106
Circular map for MR207106

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