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Fads2 (NM_019699) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207091
Fads2 (Myc-DDK-tagged) - Mouse fatty acid desaturase 2 (Fads2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fads2
Synonyms 2900042M13Rik; Fadsd2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207091 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGGGAGGTAACCAGGGAGAGGGGAGCACCGAGCGCCAGGCTCCGATGCCCACCTTCCGTTGGG
AGGAGATTCAGAAGCACAACCTGCGCACCGACCGGTGGCTCGTCATCGACCGCAAGGTCTACAACGTTAC
CAAATGGTCCCAGCGGCACCCGGGGGGCCACCGTGTCATCGGACACTATTCGGGAGAAGATGCTACGGAT
GCCTTCCGTGCCTTCCATCTGGACCTGGACTTCGTGGGCAAGTTCTTGAAGCCCCTGCTGATTGGTGAGC
TGGCCCCAGAGGAGCCCAGCCTGGACCGTGGCAAAAGCTCTCAGATCACCGAGGACTTCAGGGCCCTGAA
GAAGACTGCTGAGGACATGAACCTCTTCAAAACCAACCACCTGTTCTTCTTTCTCCTCCTGTCCCACATC
ATCGTCATGGAAAGCCTTGCCTGGTTCATCCTCTCGTACTTCGGCACTGGCTGGATTCCTACCCTCGTCA
CAGCCTTTGTCCTCGCTACCTCTCAGGCCCAAGCTGGATGGCTGCAACATGACTATGGCCACCTTTCTGT
CTATAAGAAATCCATATGGAACCACGTTGTCCACAAGTTTGTCATTGGCCACTTAAAGGGTGCCTCAGCC
AACTGGTGGAACCACCGACATTTCCAACACCATGCCAAGCCCAACATCTTCCACAAGGACCCGGACATAA
AGAGCCTGCATGTGTTTGTCCTTGGCGAGTGGCAGCCCCTTGAGTATGGCAAGAAGAAGCTGAAATACCT
GCCCTACAACCACCAGCATGAATACTTCTTCCTGATCGGACCGCCGCTGCTCATCCCTATGTACTTCCAG
TACCAGATCATCATGACAATGATCAGCCGCAGGGACTGGGTGGACTTGGCTTGGGCCATCAGCTACTATA
TGCGTTTCTTCTACACCTACATCCCTTTCTACGGCATCTTGGGAGCCCTGGTTTTCCTCAACTTTATCAG
GTTCCTGGAGAGCCACTGGTTTGTGTGGGTCACACAGATGAACCACCTTGTCATGGAGATTGATCTTGAT
CACTACCGGGACTGGTTCAGCAGCCAGCTGGCAGCCACCTGCAATGTGGAGCAGTCCTTCTTCAATGACT
GGTTCAGCGGGCACCTCAATTTCCAGATTGAGCACCACCTCTTCCCCACTATGCCACGTCACAACCTGCA
CAAGATTGCCCCACTGGTGAAGTCTCTCTGCGCCAAGCATGGCATTGAATACCAGGAGAAGCCGTTGCTG
AGGGCCCTGATCGACATTGTGAGTTCACTGAAGAAGTCTGGGGAGCTGTGGCTGGATGCTTACCTCCATA
AA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207091 protein sequence
Red=Cloning site Green=Tags(s)

MGKGGNQGEGSTERQAPMPTFRWEEIQKHNLRTDRWLVIDRKVYNVTKWSQRHPGGHRVIGHYSGEDATD
AFRAFHLDLDFVGKFLKPLLIGELAPEEPSLDRGKSSQITEDFRALKKTAEDMNLFKTNHLFFFLLLSHI
IVMESLAWFILSYFGTGWIPTLVTAFVLATSQAQAGWLQHDYGHLSVYKKSIWNHVVHKFVIGHLKGASA
NWWNHRHFQHHAKPNIFHKDPDIKSLHVFVLGEWQPLEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQ
YQIIMTMISRRDWVDLAWAISYYMRFFYTYIPFYGILGALVFLNFIRFLESHWFVWVTQMNHLVMEIDLD
HYRDWFSSQLAATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLL
RALIDIVSSLKKSGELWLDAYLHK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019699
ORF Size 1332 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019699.2
RefSeq Size 1508 bp
RefSeq ORF 1335 bp
Locus ID 56473
UniProt ID Q9Z0R9
Cytogenetics 19 A
MW 52.4 kDa
Summary Acts as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively (PubMed:9867867). Subsequently, in the biosynthetic pathway of HUFA n-3 series, desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA)(22:6n-3), an important lipid for nervous system function (By similarity). Desaturates palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum (By similarity). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).UniProtKB/Swiss-Prot Function
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"NM_019699" in other vectors (3)
SKU Description Size Price
MG207091 Fads2 (tGFP-tagged) - Mouse fatty acid desaturase 2 (Fads2) 10 ug
$657.00
MR207091L3 Lenti ORF clone of Fads2 (Myc-DDK-tagged) - Mouse fatty acid desaturase 2 (Fads2) 10 ug
$757.00
MR207091L4 Lenti ORF clone of Fads2 (mGFP-tagged) - Mouse fatty acid desaturase 2 (Fads2) 10 ug
$757.00

Plasmid Map for MR207091

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207091
Circular map for MR207091

file-alt Citations

Chaperone-mediated autophagy sustains haematopoietic stem-cell function
Dong, Wang, Kao et al
Nature (2021)
Cited by 286
AI Summary: OriGene's pCMV6-Entry-FADS2-Myc-DDK plasmid (MR207091) was used in this study to investigate the role of FADS2 in chaperone-mediated autophagy and haematopoietic stem cell function.
Snippet: The pCMV6-Entry-FADS2-Myc-DDK plasmid was purchased from Origene (MR207091) and single point mutation of FADS2 42K to Q and A was performed at GenScript
Showing 1-1 of 1 papers.
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