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Nfix (NM_001081982) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207035
Nfix (Myc-DDK-tagged) - Mouse nuclear factor I/X (Nfix), transcript variant 1
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nfix
Synonyms CTF; NF-I/X; NF1-X; NFI-X
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207035 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACTCCCCGTACTGCCTCACCCAGGATGAGTTCCACCCGTTTATCGAGGCGCTGCTGCCTCACGTCC
GAGCCTTCTCCTACACCTGGTTCAACCTGCAGGCGCGGAAGCGCAAGTACTTCAAGAAGCACGAGAAGCG
GATGTCAAAGGACGAGGAGCGCGCAGTGAAGGACGAGCTGCTGGGCGAGAAGCCTGAGATCAAGCAGAAG
TGGGCATCCCGGCTGTTGGCCAAGCTGCGCAAAGACATCCGGCCCGAGTTCCGCGAGGACTTTGTGCTAA
CCATCACGGGCAAGAAGCCCCCCTGCTGCGTGCTTTCCAACCCCGACCAGAAGGGCAAGATCCGGCGGAT
TGACTGCCTGCGCCAGGCTGACAAGGTGTGGCGGCTGGACCTGGTCATGGTGATTTTGTTTAAAGGGATC
CCTTTGGAAAGTACTGATGGGGAGCGGCTCTACAAGTCGCCCCAGTGCTCGAACCCCGGCCTGTGTGTCC
AGCCACATCACATTGGAGTCACAATCAAAGAACTGGACCTTTATCTGGCTTACTTTGTCCACACTCCGGA
ATCCGGACAATCAGATAGTTCAAACCAGCAAGGAGATGCGGACATCAAACCACTGCCCAACGGGCACTTA
AGTTTCCAGGACTGCTTTGTGACGTCTGGGGTCTGGAATGTGACAGAGCTGGTGAGAGTATCACAGACTC
CAGTTGCGACTGCATCAGGGCCCAACTTCTCACTGGCGGACCTGGAGAGCCCCAGCTACTACAACATAAA
TCAAGTGACCCTGGGAAGGCGGTCCATCACCTCCCCTCCTTCCACCAGCAGCACCAAGCGCCCCAAGTCC
ATCGACGACAGTGAGATGGAGAGTCCAGTAGATGATGTGTTCTATCCTGGGACAGGCCGCTCTCCGGCCG
CTGGCAGCAGCCAGTCTAGCGGATGGCCCAATGACGTGGATGCAGGCCCTGCTTCTCTAAAGAAGTCAGG
AAAGCTGGACTTCTGCAGCGCCCTCTCCTCTCAGGGCAGTTCCCCACGCATGGCTTTCACCCACCACCCG
CTGCCTGTGCTTGCTGGAGTCAGACCAGGGAGCCCCCGGGCCACGGCATCAGCGCTGCACTTCCCTTCCA
CGTCCATCATCCAGCAGTCGAGCCCGTATTTCACACACCCAACCATCCGCTACCACCACCACCATGGGCA
GGACTCGCTGAAGGAGTTTGTGCAGTTTGTGTGCTCTGACGGCTCGGGTCAGGCCACCGGACAGCATTCA
CAACGACAGGCGCCTCCTCTGCCAACCGGTTTGTCAGCATCGGATCCCGGGACGGCAACTTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207035 protein sequence
Red=Cloning site Green=Tags(s)

MYSPYCLTQDEFHPFIEALLPHVRAFSYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEIKQK
WASRLLAKLRKDIRPEFREDFVLTITGKKPPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGI
PLESTDGERLYKSPQCSNPGLCVQPHHIGVTIKELDLYLAYFVHTPESGQSDSSNQQGDADIKPLPNGHL
SFQDCFVTSGVWNVTELVRVSQTPVATASGPNFSLADLESPSYYNINQVTLGRRSITSPPSTSSTKRPKS
IDDSEMESPVDDVFYPGTGRSPAAGSSQSSGWPNDVDAGPASLKKSGKLDFCSALSSQGSSPRMAFTHHP
LPVLAGVRPGSPRATASALHFPSTSIIQQSSPYFTHPTIRYHHHHGQDSLKEFVQFVCSDGSGQATGQHS
QRQAPPLPTGLSASDPGTATF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081982
ORF Size 1323 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081982.3
RefSeq Size 5626 bp
RefSeq ORF 1326 bp
Locus ID 18032
UniProt ID P70257
Cytogenetics 8 41.02 cM
MW 48.9 kDa
Summary Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081982" in other vectors (3)
SKU Description Size Price
MC200098 Nfix (untagged) - Mouse nuclear factor I/X (Nfix), transcript variant 1, (10ug) 10 ug
$457.00
MR207035L3 Lenti ORF clone of Nfix (Myc-DDK-tagged) - Mouse nuclear factor I/X (Nfix), transcript variant 1 10 ug
$757.00
MR207035L4 Lenti ORF clone of Nfix (mGFP-tagged) - Mouse nuclear factor I/X (Nfix), transcript variant 1 10 ug
$757.00

Plasmid Map for MR207035

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207035
Circular map for MR207035

file-alt Citations

High-mobility group AT-Hook 1 mediates the role of nuclear factor I/X in osteogenic differentiation through activating canonical Wnt signaling
Wu, Wang, Shan et al
Stem Cells (2021)
Cited by 13
AI Summary: OriGene contributed the Nfix expression construct used in this study to investigate the role of NFIX in osteogenic differentiation.
Snippet: and visualized using a chemiluminescence reagent (Proteintech). 2.4 | Constructs and transfections The Nfix expression construct was obtained from Origene (Beijing, China). The translated region of Hmga1 cDNA was PCR;- and visualized using a chemiluminescence reagent (Proteintech). 2.4 | Constructs and transfections The Nfix expression construct was obtained from Origene (Beijing, China). …
Showing 1-1 of 1 papers.
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