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Gpr177 (BC018381) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207034
Gpr177 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 177 (cDNA clone MGC:25946 IMAGE:4237619)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Other products for "Gpr177 or BC018381"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr177
Synonyms 5031439A09Rik; AI173978; AI987742; Gpr177
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207034 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATGAGTCCATGGTTCCAGTTTATGCTGTTTATCTTGCAGCTAGACATTGCTTTCAAGCTGAACA
ACCAAATCAGAGAAAATGCAGAAATTTCCATGGATGTTTCCCTGGGTTACCGTGATGATATGTTTTCTGA
ATGGACTGAAATGGCCCATGAAAGAGTACCACGTAAACTCAAATGTACTTTTACATCCCCCAAGACCCCA
GAGCACGAAGGTCGTTATTATAATTGTGATGTCCTTCCTTTCATGGAAATTGGGTCAGTGGCTCATAAGT
ATTACCTTCTAAATATCCGGCTACCTGTAAATGAGAAAAAGAAAATCAATGTTGGAATTGGGGAGATAAA
GGACATTCGGTTGGTGGGAATCCACCAAAACGGAGGTTTCACTAAGGTATGGTTTGCTATGAAGACCTTC
CTTACACCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGATGTCCCGACCTCCAG
TGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCTGTGGAATGGTT
TTCTATTGGATTTGACTGGACCTGGATGCTGTTATTTGGTGACATCCGACAGGGCATCTTCTATGCAATG
CTTCTTTCTTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAACATGAACGGAATCACATTGCAG
GGTACTGGAAGCAAGTTGGACCAATTGCTGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGAAAG
AGGAGTACAACTCACAAATCCTTTCTACAGTATCTGGACTACAGATGTTGGAACAGAACTGGCTATGGCT
TTCATCATTGTGGCAGGTATCTGCCTCTGCCTCTATTTCCTGTTTCTGTGCTTCATGGTATTTCAGGTAT
TTAGAAACATCAGTGGGAAGCAGTCTAGCCTCCCAGCCATGAGCAAAGTCCGGAGGCTGCACTATGAGGG
TCTGATCTTCAGGTTCAAGTTCCTCATGCTTATCACCTTGGCTTGTGCTGCCATGACTGTTATCTTCTTC
ATCGTTAGTCAGGTGTCAGAAGGCCATTGGAAATGGGGTGGTGTCACAGTTCAAGTGAGCAGTGCTTTCT
TCACTGGAATCTATGGGATGTGGAACCTGTATGTCTTTGCTTTGATGTTCTTGTATGCACCATCCCATAA
GAACTATGGGGAAGACCAGTCTAATGGTGACCTGGGTGTCCACAGCGGGGAAGAACTGCAGCTTACTACC
ACTATAACCCATGTAGATGGACCAACTGAGATCTACAAGTTGACCCGTAAAGAAGCACAGGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207034 protein sequence
Red=Cloning site Green=Tags(s)

MEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKLKCTFTSPKTP
EHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTF
LTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAM
LLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMA
FIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFF
IVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTT
TITHVDGPTEIYKLTRKEAQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC018381
ORF Size 1323 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC018381, AAH18381
RefSeq Size 2188 bp
RefSeq ORF 1325 bp
Locus ID 68151
Cytogenetics 3 H4
MW 51.3 kDa
Summary Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.UniProtKB/Swiss-Prot Function
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Documents
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"BC018381" in other vectors (4)
SKU Description Size Price
MC217851 Gpr177 (untagged) - Mouse G protein-coupled receptor 177 (cDNA clone MGC:25946 IMAGE:4237619), (10ug) 10 ug
$503.00
MG207034 Gpr177 (tGFP-tagged) - Mouse G protein-coupled receptor 177 (cDNA clone MGC:25946 IMAGE:4237619) 10 ug
$657.00
MR207034L3 Lenti ORF clone of Gpr177 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 177 (cDNA clone MGC:25946 IMAGE:4237619) 10 ug
$757.00
MR207034L4 Lenti ORF clone of Gpr177 (mGFP-tagged) - Mouse G protein-coupled receptor 177 (cDNA clone MGC:25946 IMAGE:4237619) 10 ug
$757.00

Plasmid Map for MR207034

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207034
Circular map for MR207034

file-alt Citations

Palmitoylation regulates epidermal homeostasis and hair follicle differentiation
Mill, Lee, Fukata et al
PLoS Genet (2009) 5 (11), e1000748 DOI: 10.1371/journal.pgen.1000748
Cited by 109
AI Summary: In this study, OriGene products—including the Zdhhc21 cDNA clone (OriGene, MC202414) and Zdhhc21 shRNA plasmids (OriGene, TL513593)—were used to manipulate and analyze Zdhhc21 gene expression, helping to elucidate the role of palmitoylation in hair follicle differentiation and epidermal homeostasis.
FigureFigureFigure
Showing 1-1 of 1 papers.
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