Wipf2 (NM_197940) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207014
Wipf2 (Myc-DDK-tagged) - Mouse WAS/WASL interacting protein family, member 2 (Wipf2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wipf2
Synonyms 1110014J05Rik; 5730509C05Rik; AA407487; Gm1176; Wich; Wire
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207014 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAATTCCTCCTCCCCCTCCGCCCCCGCCTGGTCCTCCCCCACCTCCCACATTTAATCAGGCAAACA
CAGAACAGCCCAAACTCAGTAGAGATGAGCAGCGGAATCGAGGCGCCCTCTTACAAGACATTTGCAAAGG
GACCAAACTAAAGAAGGTGACCAACGTTAATGATCGGAGTGCTCCTGTCATCGAGAAGCCCAGAGGGAGC
AGTGGTGGCTACGGCCCCGGAGCTGCTGCTCTGCAGCCCAAAGGAGGTCTCTTTCAAGGAGGAGTGCCGA
AGCTCCGACCTGTGGGAGCCAAGGATGCTTCAGAGGCTCCAGCTGGTAAGCCGGCCCTGCAAGTCCCCAG
CTCTCGAGCTGCTGCTCCAAGGCCTCCGGGGTCTGCAGCCAGTGGGCGTCCTCACGATGATACAGACAGC
AACCGAGCCTCCCTCCCAGAACTGCCCCGGATGCAGAGACCCTCCTTACCGGACCTCTCTAGGCCCAACA
CCGCCAGTGGCACAGGCATGAAGCACAGCTCCTCTGCACCTCCCCCACCACCTCCAGGGCGGCGTGCCAA
CGCGCCCCCCACCCCTCTGCCTCTGCACAGCAACAAAGCCCAAGCCTACAACAGGGAGAAACCATTGCCT
CCAACACCTGGACAGAGGCTGCACCCTGGACGAGAAGGACATCCTGCTCCACCCCCTGTAAAGCCACCTC
CTTCCCCTGTGAACATCAGAACAGGACCCAGTGGCCAGTCTCTGGCTCCTCCCCCACCGCCTTACCGCCA
GCCTCCTGGGGTCCCCAATGGACCCTCAAGCCCCACCAATGAGTCAGCCCCTGAGCTGCCACAGAGACAC
AATTCTTTGCATAGGAAAACGCCAGGGCCCGTCAGAGGCCTTGCACCTCCTCCACCCACCTCAGCCACCC
CCTCCTTGTTGAGTAACAGGCCACCTCCCCCAGCCCGAGAGCCTCCTAGCAGGGGAGCAGCTCCCCCGCC
CCCACCGCCCATGATCCGAAATGGTGCCAGGGATGCTCCCCCTCCTCCCCCACCTTACCGGATGCATGGG
TCAGAGCCACCAAGCAGAGGAAAGCCCCCACCTCCACCCTCCAGGACACCAGCTGGGCCGCCCCCTCCCC
CACCACCCCCCTTGCGGAATGGCCATAGAGACTCCATCACCACTGTCCGCTCCTTCTTGGATGACTTTGA
ATCCAAGTACTCCTTCCATCCAGTGGAGGACTTCCCTGCTCCAGAAGAATATAAACATCTTCAAAGAGTA
TACCCCAGCAAAACAAACCGAGCTGCCCGTGGAGCCCCACCTCTCCCACCCATTCTCAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207014 protein sequence
Red=Cloning site Green=Tags(s)

MPIPPPPPPPPGPPPPPTFNQANTEQPKLSRDEQRNRGALLQDICKGTKLKKVTNVNDRSAPVIEKPRGS
SGGYGPGAAALQPKGGLFQGGVPKLRPVGAKDASEAPAGKPALQVPSSRAAAPRPPGSAASGRPHDDTDS
NRASLPELPRMQRPSLPDLSRPNTASGTGMKHSSSAPPPPPPGRRANAPPTPLPLHSNKAQAYNREKPLP
PTPGQRLHPGREGHPAPPPVKPPPSPVNIRTGPSGQSLAPPPPPYRQPPGVPNGPSSPTNESAPELPQRH
NSLHRKTPGPVRGLAPPPPTSATPSLLSNRPPPPAREPPSRGAAPPPPPPMIRNGARDAPPPPPPYRMHG
SEPPSRGKPPPPPSRTPAGPPPPPPPPLRNGHRDSITTVRSFLDDFESKYSFHPVEDFPAPEEYKHLQRV
YPSKTNRAARGAPPLPPILR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_197940
ORF Size 1320 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_197940.2
RefSeq Size 7589 bp
RefSeq ORF 1323 bp
Locus ID 68524
UniProt ID Q6PEV3
Cytogenetics 11 D
MW 46.3 kDa
Summary Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_197940" in other vectors (3)
SKU Description Size Price
MG207014 Wipf2 (tGFP-tagged) - Mouse WAS/WASL interacting protein family, member 2 (Wipf2) 10 ug
$657.00
MR207014L3 Lenti ORF clone of Wipf2 (Myc-DDK-tagged) - Mouse WAS/WASL interacting protein family, member 2 (Wipf2) 10 ug
$757.00
MR207014L4 Lenti ORF clone of Wipf2 (mGFP-tagged) - Mouse WAS/WASL interacting protein family, member 2 (Wipf2) 10 ug
$757.00

Plasmid Map for MR207014

Download sequence for

Cloning scheme for MR207014

Circular map for MR207014

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