Cdca7l (NM_146040) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206981
Cdca7l (Myc-DDK-tagged) - Mouse cell division cycle associated 7 like (Cdca7l)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdca7l
Synonyms BC006933; JPO2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206981 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGGCCACGCGCTCCCAGATCCCCAAAGAAGTAGCTGACATCTTCAGTGCTCCCAGTGACGATG
AAGAGTTTGTGGGTTTCCAGGATGATGTTCCCATGCAAAACCTGTCAGAGAGCTGTGGTAGTTTGGACTC
ACGGGAGTTGGAGAAACAGCAGAATGTGTGCTTCCGTTCCAAATACTTCACAGAAGAACTAAGAAGGATT
TTCAAAGAGGACACGGACTCAGAAATGGAAGATTTTGAAGGATTTACAGAGAGCGAGCTGAACATGAGCA
GTAACCCAGAGCTCATGGAGTCGGAGCTGAGTGACAGTGACAAAGCATACCCTGTGATGAATGATGCGGA
GGAAGATGATGAAGAGGAGGCTGCACCTAGAAGGGGCAGGTCCACAAGAAGAAGCAGTTTCGGTCTTCGG
GTAGCCTTTCAGTTCCCCACCAAAAAATTGGCAAGAACACCAGATAAAGATAGCTCACACTTGCTGGACA
GTAAGACGGATCTTAGAAGAAAGAAGAGCTCCAGACAGCCCAAGGGAAAGGAGGACTCTGCTTCTGATGC
CGAGGATGAGTCCAGAGCTGAGAGCCAAGAGAATTCGGATGCTCTGCTGAAAAGGGCCATGAACATCAAA
GAGAACAAGGCTATGCTTGCTCAGTTGTTGGCGGAATTGAATTCGGTGCCAGATTTCTTCCCTGTTCGGA
CACCACCTTCGGCTTCTAGGAGGCGAACACCACGACGGGCCTTCTCAGAGGGACAGATCACACGGCGCAT
GAACCCAACCCGGAGTGCACGACCCCCTGAAAAGTTTGCTCTGGAGAACTTCACCTTCTCAGCCACCAAG
CTTACTGAAGAGCTTTACAGTTTCAGAAGAAGAAAGACAATCAGTGGGGGGAAATGCCAGACGTATAGAC
GTCATCGGATATCTTCTTTCCGCTCAGTGAAAGACATCACTGAGGAAGACTTGGAAAACATTGCCATAAC
TGTGCGTGATAAAGTCTATGATAAAGTGCTTGGTAACACGTGCCATCAGTGCCGGCAGAAGACCATCGAT
ACCAAGACGGTCTGTCGGAACCAGAGCTGTGGTGGTGTGCGGGGACAGTTCTGTGGGCCCTGCCTGCGGA
ATCGCTATGGGGAGGATGTGCGGACAGCGTTGCTGGATCCGAAGTGGACATGTCCTCCCTGCCGTGGCAT
CTGCAATTGCAGTTACTGCCGGAGGCGAGATGGCCGCTGTGCTACTGGAATTCTCATTCATCTGGCCAAG
TTCTACGGTTATGATAATGTCAAGGAGTACCTGGAGAGCTTACAGAAGCAGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206981 protein sequence
Red=Cloning site Green=Tags(s)

MELATRSQIPKEVADIFSAPSDDEEFVGFQDDVPMQNLSESCGSLDSRELEKQQNVCFRSKYFTEELRRI
FKEDTDSEMEDFEGFTESELNMSSNPELMESELSDSDKAYPVMNDAEEDDEEEAAPRRGRSTRRSSFGLR
VAFQFPTKKLARTPDKDSSHLLDSKTDLRRKKSSRQPKGKEDSASDAEDESRAESQENSDALLKRAMNIK
ENKAMLAQLLAELNSVPDFFPVRTPPSASRRRTPRRAFSEGQITRRMNPTRSARPPEKFALENFTFSATK
LTEELYSFRRRKTISGGKCQTYRRHRISSFRSVKDITEEDLENIAITVRDKVYDKVLGNTCHQCRQKTID
TKTVCRNQSCGGVRGQFCGPCLRNRYGEDVRTALLDPKWTCPPCRGICNCSYCRRRDGRCATGILIHLAK
FYGYDNVKEYLESLQKQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146040
ORF Size 1314 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146040.1, NP_666152.1
RefSeq Size 2626 bp
RefSeq ORF 1317 bp
Locus ID 217946
UniProt ID Q922M5
Cytogenetics 12 F2
MW 50.2 kDa
Summary Plays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. Plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells (By similarity). Involved in apoptotic signaling pathways; May act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_146040" in other vectors (4)
SKU Description Size Price
MC205616 Cdca7l (untagged) - Mouse cell division cycle associated 7 like (Cdca7l), (10ug) 10 ug
$457.00
MG206981 Cdca7l (tGFP-tagged) - Mouse cell division cycle associated 7 like (Cdca7l) 10 ug
$657.00
MR206981L3 Lenti ORF clone of Cdca7l (Myc-DDK-tagged) - Mouse cell division cycle associated 7 like (Cdca7l) 10 ug
$757.00
MR206981L4 Lenti ORF clone of Cdca7l (mGFP-tagged) - Mouse cell division cycle associated 7 like (Cdca7l) 10 ug
$757.00

Plasmid Map for MR206981

Download sequence for

Cloning scheme for MR206981

Circular map for MR206981

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