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Sept8 (NM_033144) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206850
41525 (Myc-DDK-tagged) - Mouse septin 8 (Sept8)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sept8
Synonyms AW046166; Sepl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206850 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCACCGACTTGGAACGCGTCTCGAACGCAGAGCCTGAGCCCCGGAGCCTGTCCCTGGGCGGCC
ATGTTGGGTTTGACAGCCTCCCTGACCAGCTGGTCAGCAAGTCAGTCACTCAGGGCTTCAGCTTCAACAT
ACTCTGTGTGGGCGAAACTGGGATTGGCAAGTCCACGCTAATGAACACACTCTTCAACACGACCTTTGAG
ACTGAAGAAGCCAGTCACCATGAAGAGTGTGTGCGCCTGCGGCCTCAGACCTATGACCTCCAAGAGAGTA
ACGTTCATCTCAAGCTGACCATCGTGGATGCTGTGGGCTTTGGCGATCAGATCAATAAGGATGACAGTTA
CAGGCCCATAGTTGACTACATCGACGCGCAGTTTGAAAACTATCTGCAGGAGGAGTTGAAGATCCGCCGT
TCCCTCTTTGACTATCACGACACGAGGATCCATGTTTGCCTCTACTTCATCACGCCCACCGGGCACTCCC
TGAAGTCCCTGGATCTGGTGACCATGAAGAAACTAGATAGCAAGGTGAATATAATCCCCATCATTGCCAA
GGCTGACACCATCTCCAAGAGCGAGCTCCACAAGTTCAAAATCAAGATCATGGGTGAGCTGGTCAGCAAC
GGAGTCCAGATCTACCAGTTTCCCACCGATGACGAGGCTGTCGCCGAGATTAATGCAGTCATGAACGCAC
ACCTGCCTTTTGCCGTGGTGGGCAGCACAGAGGAGGTGAAGGTGGGGAATAAGCTGGTTCGAGCACGACA
GTACCCTTGGGGTGTGGTGCAGGTGGAGAATGAGAATCACTGTGACTTCGTGAAGCTGCGGGAGATGCTG
ATCCGGGTGAACATGGAGGACCTGCGCGAGCAGACCCACAGCCGGCACTACGAGCTCTACCGCCGCTGCA
AGTTGGAGGAGATGGGCTTCCAGGACAGTGACGGTGACAGCCAGCCCTTCAGCCTCCAAGAGACATATGA
GGCAAAGAGGAAGGAGTTCCTAAGCGAGCTGCAGAGGAAGGAGGAAGAGATGCGACAGATGTTTGTCAAC
AAAGTCAAGGAGACAGAGCTTGAGCTGAAGGAGAAGGAACGGGAGCTCCATGAGAAGTTTGAGCACCTGA
AGCGAATCCACCAGGAGGAGAAGCGCAAGGTAGAGGAGAAGCGCAGGGAGCTGGAAGAGGAGACCAACGC
CTTCAACTGCCGGAAGGCGGCCATGGAGGCCCTGCAGTCACAGGCCTTGCATGCCACTTCACAGCAGCCT
CTGAGGAAAGACAAGGACAAGAAGAAATTT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206850 protein sequence
Red=Cloning site Green=Tags(s)

MAATDLERVSNAEPEPRSLSLGGHVGFDSLPDQLVSKSVTQGFSFNILCVGETGIGKSTLMNTLFNTTFE
TEEASHHEECVRLRPQTYDLQESNVHLKLTIVDAVGFGDQINKDDSYRPIVDYIDAQFENYLQEELKIRR
SLFDYHDTRIHVCLYFITPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADTISKSELHKFKIKIMGELVSN
GVQIYQFPTDDEAVAEINAVMNAHLPFAVVGSTEEVKVGNKLVRARQYPWGVVQVENENHCDFVKLREML
IRVNMEDLREQTHSRHYELYRRCKLEEMGFQDSDGDSQPFSLQETYEAKRKEFLSELQRKEEEMRQMFVN
KVKETELELKEKERELHEKFEHLKRIHQEEKRKVEEKRRELEEETNAFNCRKAAMEALQSQALHATSQQP
LRKDKDKKKF

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_033144
ORF Size 1290 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033144.2
RefSeq Size 4647 bp
RefSeq ORF 1293 bp
Locus ID 20362
UniProt ID Q8CHH9
Cytogenetics 11 31.96 cM
MW 50 kDa
Summary Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion (By similarity). Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_033144" in other vectors (3)
SKU Description Size Price
MC206044 41525 (untagged) - Mouse septin 8 (Sept8), (10ug) 10 ug
$457.00
MR206850L3 Lenti ORF clone of 41525 (Myc-DDK-tagged) - Mouse septin 8 (Sept8) 10 ug
$757.00
MR206850L4 Lenti ORF clone of 41525 (mGFP-tagged) - Mouse septin 8 (Sept8) 10 ug
$757.00

Plasmid Map for MR206850

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206850
Circular map for MR206850

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