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Kynu (BC069848) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206825
Kynu (Myc-DDK-tagged) - Mouse kynureninase (L-kynurenine hydrolase) (cDNA clone MGC:78020 IMAGE:5097145)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kynu
Synonyms 4432411A05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206825 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAGCCTTCGCCTCTTGAGCTTCCAGTTGATGCAGTGCGGCGCATCGCGGCTGAACTCAATTGTG
ACCCAACAGATGAGAGGGTTGCTCTCCGCTTGGATGAGGAAGATAAACTGAGTCATTTTAGGAACTGTTT
TTATATTCCCAAAATGCGGGACCTGCCTTCAATTGATCTATCTTTAGTGAGTGAGGATGATGATGCCATC
TATTTCCTGGGAAATTCCCTTGGCCTTCAACCGAAAATGGTTAGGACATACCTGGAGGAAGAACTAGATA
AGTGGGCCAAGATGGGAGCCTATGGCCATGATGTAGGCAAACGCCCTTGGATTGTAGGGGATGAGAGTAT
TGTAAGCCTTATGAAGGACATTGTAGGAGCCCATGAGAAAGAAATAGCTCTAATGAATGCTTTGACTATT
AATTTACATCTCCTGCTGTTATCATTCTTTAAGCCTACTCCAAAGCGGCACAAAATTCTTCTAGAAGCCA
AAGCCTTCCCTTCTGATCATTATGCTATTGAGTCACAGATTCAACTTCACGGACTTGATGTTGAGAAAAG
TATGCGGATGGTAAAGCCACGAGAGGGGGAAGAGACCTTAAGGATGGAGGACATACTGGAAGTAATCGAG
GAGGAAGGAGACTCGATCGCCGTGATCCTGTTCAGTGGGCTGCACTTTTATACTGGACAGCTGTTCAACA
TTCCTGCCATAACAAAAGCTGGACATGCAAAGGGCTGTTTTGTTGGCTTTGACCTAGCACATGCAGTTGG
AAATGTTGAACTCCGCTTACATGACTGGGGTGTTGACTTTGCCTGCTGGTGTTCCTATAAGTATTTAAAT
TCAGGAGCTGGAGGTCTGGCTGGTGCCTTTGTCCACGAGAAACATGCTCATACTGTCAAGCCTGCGTTAG
TGGGATGGTTCGGCCATGACCTCAGTACAAGGTTTAACATGGATAACAAACTACAATTAATCCCCGGGGC
CAATGGATTCCGAATTTCAAACCCTCCCATTTTGTTGGTCTGCTCCTTGCACGCCAGTTTAGAGGTCTTT
CAGCAAGCAACTATGACTGCGCTGAGAAGAAAATCCATTCTGCTGACAGGTTATCTGGAATACATGCTCA
AACATTACCACAGCAAAGATAACACCGAAAACAAGGGGCCGATTGTGAATATCATCACCCCGTCCAGAGC
AGAGGAGCGTGGCTGCCAGTTAACACTCACCTTTTCCATTCCCAAGAAAAGCGTTTTTAAGGAACTAGAA
AAAAGAGGAGTCGTTGTATGTATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206825 protein sequence
Red=Cloning site Green=Tags(s)

MMEPSPLELPVDAVRRIAAELNCDPTDERVALRLDEEDKLSHFRNCFYIPKMRDLPSIDLSLVSEDDDAI
YFLGNSLGLQPKMVRTYLEEELDKWAKMGAYGHDVGKRPWIVGDESIVSLMKDIVGAHEKEIALMNALTI
NLHLLLLSFFKPTPKRHKILLEAKAFPSDHYAIESQIQLHGLDVEKSMRMVKPREGEETLRMEDILEVIE
EEGDSIAVILFSGLHFYTGQLFNIPAITKAGHAKGCFVGFDLAHAVGNVELRLHDWGVDFACWCSYKYLN
SGAGGLAGAFVHEKHAHTVKPALVGWFGHDLSTRFNMDNKLQLIPGANGFRISNPPILLVCSLHASLEVF
QQATMTALRRKSILLTGYLEYMLKHYHSKDNTENKGPIVNIITPSRAEERGCQLTLTFSIPKKSVFKELE
KRGVVVCI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC069848
ORF Size 1284 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC069848, AAH69848
RefSeq Size 1599 bp
RefSeq ORF 1286 bp
Locus ID 70789
Cytogenetics 2 B
MW 48.2 kDa
Summary Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.UniProtKB/Swiss-Prot Function
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"BC069848" in other vectors (4)
SKU Description Size Price
MC218419 Kynu (untagged) - Mouse kynureninase (L-kynurenine hydrolase) (cDNA clone MGC:78020 IMAGE:5097145), (10ug) 10 ug
$503.00
MG206825 Kynu (tGFP-tagged) - Mouse kynureninase (L-kynurenine hydrolase) (cDNA clone MGC:78020 IMAGE:5097145) 10 ug
$657.00
MR206825L3 Lenti ORF clone of Kynu (Myc-DDK-tagged) - Mouse kynureninase (L-kynurenine hydrolase) (cDNA clone MGC:78020 IMAGE:5097145) 10 ug
$757.00
MR206825L4 Lenti ORF clone of Kynu (mGFP-tagged) - Mouse kynureninase (L-kynurenine hydrolase) (cDNA clone MGC:78020 IMAGE:5097145) 10 ug
$757.00

Plasmid Map for MR206825

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206825
Circular map for MR206825

file-alt Citations

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