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Nusap1 (BC004787) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206803
Nusap1 (Myc-DDK-tagged) - Mouse nucleolar and spindle associated protein 1 (cDNA clone MGC:8102 IMAGE:3588697)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Other products for "Nusap1 or BC004787"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nusap1
Synonyms LNP, ANKT, SAPL, BM037, NuSAP, Q0310
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206803 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGTCCCCTCTGCAGAGGAGCTGGACTCCTTCAAGTACAGCGACCTGCAAAATTTAGCCAAAAGGC
TGGGCCTCCGGGCTAACATGAAGGCAGACAAGTTGTTAAAAGCCTTGAAAGCACACCTGAATCCAGAAAC
AAGGAAAGAAAATAAAAATCAGGATGAAAATCAATTCTCCACTGATGAAACTGAGATACACGTTAGCAGT
GAGGAGCAAGCTGAGACGGAATCAGGTGGTCACGTCACCAAAACGAGGAGGAGGAGGAGGAAGAAGCACA
AGACCATCCATGGAATTCCTACCTCCCAGACTTTGTTGCAGGATCATTTGGAGATGAAAGGAACTGATAG
TAGTAACTTCCAAAATCAAGAAAATCAGGAAAATCAAGACCCCAGGGATACAGCAGAAGTTCCTTCTCTG
CCGGAGCAGAGGCCAGAGGACGGCAATGCGGCTTCTTCAGGAGAAGGAGAAGTAAATGACATTAAAGATT
CAAAGAAGCCTTTAGAAAAAAGATCTCTATGCACGGATGAGTTTTCTAAACTTGGGAACAATAAAAGGAC
TTCAGCCACAACACCAAACTTTAAGAAGCTTCATGAGGCTCGTTTTAAGAAAATGGAATCCATTGATGAA
TATATTATGAGGAAAAAGAAACACCTTAAAGAACACAGTTCACTTAATGAACTAAAGCTTGACAAAAAAG
GGATAGTGACCCCAGTTCCTCCAAGAGGAAGGCTCTCTGTACCCTGTACTCCTGCCAGGCAGCAGTGCCC
ACAAGGCCACTCAGCAACTAAAATGAATGTCAGGTTTTCAGCTGCTACTAAAGACAATGAACATAAGTGC
TCACTGACCAAGACACCAGCCAGAAAGTCTCCACACGTGACTGCACCTGGGAGTGCTTCAAAAGGCCAGG
CTGTGTTTAGGACACCCAAGTCAAAGGCCACTGAAAGGACTTCTATTGCAGTTATTACCCCTTTCAAGTT
GATGACTGAAGCAACACAGACTCCAAGTTCTAGTAAGAAGCCAGTATTTGATCTCAAAGCAAGCTTGTCT
CGTCCCCTCAACTACAAGCCACACAAAGGAAAGCTGAAACCTTGGGGACAAGCTAAAGAGAACAATTCTC
TGAACGAACGTGTAAGCAGAGTTACCTTCCACAGGAAAACTTACAAACAACCTCATCTCCAAACCAGGGA
AGAACGATGGAAGAGACAAGAGCAAGAACGAAAGGAGAAGAAAGAAAAGCTTTTGGAAGCTCGAAGAAAC
CTGGGTGTGACTAAAGCCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206803 protein sequence
Red=Cloning site Green=Tags(s)

MTVPSAEELDSFKYSDLQNLAKRLGLRANMKADKLLKALKAHLNPETRKENKNQDENQFSTDETEIHVSS
EEQAETESGGHVTKTRRRRRKKHKTIHGIPTSQTLLQDHLEMKGTDSSNFQNQENQENQDPRDTAEVPSL
PEQRPEDGNAASSGEGEVNDIKDSKKPLEKRSLCTDEFSKLGNNKRTSATTPNFKKLHEARFKKMESIDE
YIMRKKKHLKEHSSLNELKLDKKGIVTPVPPRGRLSVPCTPARQQCPQGHSATKMNVRFSAATKDNEHKC
SLTKTPARKSPHVTAPGSASKGQAVFRTPKSKATERTSIAVITPFKLMTEATQTPSSSKKPVFDLKASLS
RPLNYKPHKGKLKPWGQAKENNSLNERVSRVTFHRKTYKQPHLQTREERWKRQEQERKEKKEKLLEARRN
LGVTKAQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC004787
ORF Size 1281 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC004787, AAH04787
RefSeq Size 2142 bp
RefSeq ORF 1283 bp
Locus ID 108907
Cytogenetics 2 E5
MW 48.6 kDa
Summary Microtubule-associated protein with the capacity to bundle and stabilize microtubules. May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.UniProtKB/Swiss-Prot Function
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"BC004787" in other vectors (3)
SKU Description Size Price
MG206803 Nusap1 (tGFP-tagged) - Mouse nucleolar and spindle associated protein 1 (cDNA clone MGC:8102 IMAGE:3588697) 10 ug
$657.00
MR206803L3 Lenti ORF clone of Nusap1 (Myc-DDK-tagged) - Mouse nucleolar and spindle associated protein 1 (cDNA clone MGC:8102 IMAGE:3588697) 10 ug
$757.00
MR206803L4 Lenti ORF clone of Nusap1 (mGFP-tagged) - Mouse nucleolar and spindle associated protein 1 (cDNA clone MGC:8102 IMAGE:3588697) 10 ug
$757.00

Plasmid Map for MR206803

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206803
Circular map for MR206803

file-alt Citations

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