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Plin2 (NM_007408) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206783
Plin2 (Myc-DDK-tagged) - Mouse perilipin 2 (Plin2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Plin2 or NM_007408"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Plin2
Synonyms AA407157; Adfp; ADPH; Adrp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007408
ORF Size 1275 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007408.3, NP_031434.3
RefSeq Size 1887 bp
RefSeq ORF 1278 bp
Locus ID 11520
UniProt ID P43883
Cytogenetics 4 40.69 cM
MW 47.1 kDa
Summary May be involved in development and maintenance of adipose tissue.UniProtKB/Swiss-Prot Function
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Documents
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"NM_007408" in other vectors (4)
SKU Description Size Price
MC202691 Plin2 (untagged) - Mouse perilipin 2 (Plin2), (10ug) 10 ug
$686.00
MG206783 Plin2 (tGFP-tagged) - Mouse adipose differentiation related protein (Adfp) 10 ug
$886.00
MR206783L3 Lenti ORF clone of Plin2 (Myc-DDK-tagged) - Mouse perilipin 2 (Plin2) 10 ug
$986.00
MR206783L4 Lenti ORF clone of Plin2 (mGFP-tagged) - Mouse perilipin 2 (Plin2) 10 ug
$986.00

Plasmid Map for MR206783

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206783
Circular map for MR206783

file-alt Citations

The major cap-binding protein eIF4E regulates lipid homeostasis and diet-induced obesity
Conn, Yang, Tom et al
Nat Metab (2021) 3 (2), 244-257 DOI: 10.1038/s42255-021-00349-z
Cited by 58
AI Summary: OriGene’s Plin2 cDNA-Flag plasmid (Cat# NM_007408) was used to transfect AML12 cells, enabling the study of lipid droplet-associated protein function in the regulation of lipid homeostasis by eIF4E.
Snippet: … A hallmark of disease progression is the ectopic deposition of lipids in the form of lipid droplets in vital organs such as the liver. However;AML12 cells were transfected with plasmid containing Plin2 cDNA tagged with Flag (no. NM_007408, ORIGENE) using Lipofectamine LTX with Plus Reagent (no. …
Structural and Functional Assessment of Perilipin 2 Lipid Binding Domains(s)
Najt, Lwande, McIntosh et al
Biochemistry (2014)
Cited by 61
AI Summary: OriGene’s pQE9-His vector was used to clone the full coding sequence of mouse Plin2 cDNA, enabling the expression of recombinant Plin2 and its mutants for structural and functional analyses in this study.
Snippet: Plasmids. The full coding sequence of mouse Plin2 cDNA was cloned into pQE9-His vector (Origene, Rockville, MD) following standard procedures. Unique restriction sites BamHI and HindIII were introduced by PCR amplification with the following forward and reverse ... Cite SaveSaving
Showing 1-2 of 2 papers.
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