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Rbbp7 (NM_009031) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206775
Rbbp7 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 7 (Rbbp7)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rbbp7
Synonyms AA409861; AI173248; AU019541; BB114024; mRbAp46
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206775 representing NM_009031
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAGTAAAGAGATGTTTGAAGATACTGTGGAGGAGCGTGTCATCAACGAAGAGTATAAAATCTGGA
AGAAGAATACACCGTTTCTGTATGACCTGGTTATGACCCATGCTCTTCAGTGGCCCAGTCTTACCGTTCA
GTGGCTTCCTGAAGTGACTAAACCAGAAGGAAAGGATTATGCCCTTCATTGGCTAGTGCTGGGCACTCAT
ACATCTGATGAGCAGAACCATCTGGTGGTTGCTCGAGTTCATATTCCCAATGATGATGCACAGTTTGATG
CTTCCCACTGTGACAGTGACAAGGGAGAATTCGGTGGCTTTGGTTCTGTAACAGGGAAAATTGAATGTGA
AATTAAAATTAACCATGAAGGAGAAGTGAATCGTGCTCGTTATATGCCACAGAATCCTCACATCATTGCC
ACAAAAACACCATCTTCTGATGTTTTGGTTTTTGACTATACAAAACACCCTGCAAAACCAGATCCAAGTG
GAGAATGTAATCCTGATCTTAGATTAAGAGGTCACCAAAAGGAAGGCTATGGTCTTTCCTGGAATTCTAA
TCTGAGTGGGCATCTCCTGAGTGCATCTGATGACCATACTGTCTGCCTGTGGGATATAAATGCAGGACCA
AAGGAAGGCAAAATTGTGGATGCTAAAGCAATCTTTACTGGCCACTCAGCTGTTGTAGAGGATGTGGCCT
GGCATCTGCTGCATGAGTCCTTGTTTGGATCTGTTGCTGATGATCAGAAACTTATGATATGGGACACCAG
ATCCAATACCACTTCTAAGCCGAGCCATTTGGTGGATGCACACACCGCTGAGGTCAACTGCCTCTCATTC
AATCCCTACAGCGAGTTCATTCTGGCAACTGGCTCTGCAGATAAGACTGTAGCTTTATGGGACCTGCGTA
ATCTGAAACTAAAACTCCACACCTTTGAATCGCATAAGGATGAAATTTTCCAGGTCCACTGGTCTCCACA
TAATGAAACTATTCTGGCCTCAAGTGGTACTGATCGCCGCCTGAATGTGTGGGATTTAAGTAAAATTGGA
GAAGAACAATCAGCAGAAGATGCAGAAGATGGGCCTCCAGAGCTCCTGTTTATTCATGGAGGGCACACTG
CCAAGATTTCTGACTTCAGCTGGAATCCCAATGAACCTTGGGTCATTTGCTCTGTGTCTGAAGATAACAT
CATGCAGATATGGCAGATGGCTGAAAATATTTACAATGATGAAGAGTCAGATGTCACGGCATCGGAACTG
GAGGGGCAAGGATCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206775 representing NM_009031
Red=Cloning site Green=Tags(s)

MASKEMFEDTVEERVINEEYKIWKKNTPFLYDLVMTHALQWPSLTVQWLPEVTKPEGKDYALHWLVLGTH
TSDEQNHLVVARVHIPNDDAQFDASHCDSDKGEFGGFGSVTGKIECEIKINHEGEVNRARYMPQNPHIIA
TKTPSSDVLVFDYTKHPAKPDPSGECNPDLRLRGHQKEGYGLSWNSNLSGHLLSASDDHTVCLWDINAGP
KEGKIVDAKAIFTGHSAVVEDVAWHLLHESLFGSVADDQKLMIWDTRSNTTSKPSHLVDAHTAEVNCLSF
NPYSEFILATGSADKTVALWDLRNLKLKLHTFESHKDEIFQVHWSPHNETILASSGTDRRLNVWDLSKIG
EEQSAEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQIWQMAENIYNDEESDVTASEL
EGQGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009031
ORF Size 1275 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009031.3, NP_033057.3
RefSeq Size 2272 bp
RefSeq ORF 1278 bp
Locus ID 245688
UniProt ID Q60973
Cytogenetics X F4
MW 48.2 kDa
Summary Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex (By similarity).UniProtKB/Swiss-Prot Function
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"NM_009031" in other vectors (3)
SKU Description Size Price
MG206775 Rbbp7 (tGFP-tagged) - Mouse retinoblastoma binding protein 7 (Rbbp7) 10 ug
$657.00
MR206775L3 Lenti ORF clone of Rbbp7 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 7 (Rbbp7) 10 ug
$757.00
MR206775L4 Lenti ORF clone of Rbbp7 (mGFP-tagged) - Mouse retinoblastoma binding protein 7 (Rbbp7) 10 ug
$757.00

Plasmid Map for MR206775

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206775
Circular map for MR206775

file-alt Citations

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