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Serpina3a (BC063325) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206715
Serpina3a (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 3A (cDNA clone MGC:74171 IMAGE:30280343), complete
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Serpina3a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206715 representing BC063325
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTCATTGCAGCTCTGGGGCTCCTGATGGTGGGGATCTGTCCTGCTGTCACCTACTGGGCAACTG
CAGATGGGCAACTGGGAGGGCACACTGCAGTCCAGAAAGACAGAGACCATGAAATACAACTGGACAGTGT
CACACTGGCTTCCATCAACACTGACTTTGCCTTCAGCCTCTACAAGAAGCTGGCTTTGGAGAATCCACAT
AAAAATATTGTCTTCTCCCCACTTAGCATCTCAGCTGCCTTGGCCCTCATGTCCCTGGGAGCAAAGGGCA
ACACCCTGGAAGAGATTCTAGAAGGTCTCAAGTTCAATCTCCCAGAGACCCCTGAGGCAGACATCCACCA
GAACTTTGGGCACCTCCTACAAATGCTCATTCAGCCAGAGAACCAGGTTCAGATCAATGCAGGCAACGCC
CTGTTTATTGATAAGCACCTGCAGATCTTGACAGAGTTCAAGGAGAAGGCAAGGGCTCTGTACAAGGCTG
AGGCCTTCACAACAGACTTCCAGCGGCCTCGTGAGGCCACAAAGCTCATCAATGACTATGTGAGGAAACA
GACCCAGGGGAAGATCAAGGAACTGGTCTCAGACCTGCATAGGAACACATCCATGGCACTAGTGAATTTC
CTCAACTTTCAAGGCTTTTGGAACGTGACTTTTGACCCTGAAGACACGTTCCTGGGAAATTTCACATTGG
ATAGGAAGAGGACAGTGAACGTGCCCATGATGAAAACCGAGGAGCTAACCACAAACTACTTCCGGGACGA
GGAGATGCAAAGCACTGTGGTGGAGCTGAATTACATAGGCAATGCCAGCTTCCTGTTCATCCTCCCTGAC
CAGGGCAGGATACAGCACGTGGAAGATAGCTTGCAACCCCAGTCTCTGAGGAAATGGAGGAAATCTTTGA
GACCCAGGATGCTAGATGAACTCTCCCTGCCCAAGTTCTCCTTATCACAAGACTACAACCTAAATGACAT
CCTTCCAGAGCTGGGTATCAAGGAAGTCTTCTCCACACAGGCTGACCTGTCTGGGATCACTGGAGCCAAA
AATATAAGAGTCTCTCAGATGATCCACCAGGCTGCGCTGGATGTTACCGAGACACACACAGAAGCAGATG
TCATCACAATAGCCCGATATAACTTCCAATCTGCAAAAATTAAGGCTAAAATTGTTAAGGTGGACAGGGA
ATTCTTGTACCTTATTTTAGACCCAATGTTTAAGAGTATTAGCGTTATGGGTAAGGTTATCAACCCTTTA
ACAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206715 representing BC063325
Red=Cloning site Green=Tags(s)

MAFIAALGLLMVGICPAVTYWATADGQLGGHTAVQKDRDHEIQLDSVTLASINTDFAFSLYKKLALENPH
KNIVFSPLSISAALALMSLGAKGNTLEEILEGLKFNLPETPEADIHQNFGHLLQMLIQPENQVQINAGNA
LFIDKHLQILTEFKEKARALYKAEAFTTDFQRPREATKLINDYVRKQTQGKIKELVSDLHRNTSMALVNF
LNFQGFWNVTFDPEDTFLGNFTLDRKRTVNVPMMKTEELTTNYFRDEEMQSTVVELNYIGNASFLFILPD
QGRIQHVEDSLQPQSLRKWRKSLRPRMLDELSLPKFSLSQDYNLNDILPELGIKEVFSTQADLSGITGAK
NIRVSQMIHQAALDVTETHTEADVITIARYNFQSAKIKAKIVKVDREFLYLILDPMFKSISVMGKVINPL
TN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC063325
ORF Size 1266 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC063325.1
RefSeq Size 1994 bp
RefSeq ORF 1268 bp
Locus ID 74069
Cytogenetics 12 E
MW 47.7 kDa
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"BC063325" in other vectors (4)
SKU Description Size Price
MC218465 Serpina3a (untagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 3A (cDNA clone MGC:74171 IMAGE:30280343), complete, (10ug) 10 ug
$503.00
MG206715 Serpina3a (tGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 3A (cDNA clone MGC:74171 IMAGE:30280343), complete 10 ug
$657.00
MR206715L3 Lenti ORF clone of Serpina3a (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 3A (cDNA clone MGC:74171 IMAGE:30280343), complete 10 ug
$757.00
MR206715L4 Lenti ORF clone of Serpina3a (mGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 3A (cDNA clone MGC:74171 IMAGE:30280343), complete 10 ug
$757.00

Plasmid Map for MR206715

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206715
Circular map for MR206715

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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