Napsa (NM_008437) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206657
Napsa (Myc-DDK-tagged) - Mouse napsin A aspartic peptidase (Napsa)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Napsa
Synonyms KAP; Kdap; NAP1; pronapsin; SNAPA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206657 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCCACTACTGCTGCTGCTGCTGTGCCTGCTGCTGGGGAATTTGGAGCCTGAGGAGGCCAAACTGA
TCCGTGTCCCTCTTCAACGAATCCACCTTGGACACAGAATCTTAAACCCACTGAATGGATGGGAACAGCT
GGCAGAGCTTTCTAGGACCTCCACCTCTGGTGGCAACCCCTCCTTTGTGCCTCTCTCCAAGTTCATGAAC
ACCCAGTATTTTGGAACTATTGGTTTGGGAACGCCTCCTCAGAATTTCACCGTTGTCTTTGACACGGGTT
CTTCCAACTTGTGGGTTCCGTCCACGAGATGTCATTTCTTCAGTTTGGCATGCTGGTTTCACCATCGCTT
TAATCCCAAGGCCTCCAGCTCCTTCAGGCCCAATGGGACCAAGTTTGCCATTCAGTATGGGACCGGGCGG
CTGAGCGGAATCCTGAGCCAGGACAATCTGACTATCGGGGGGATCCACGATGCTTTTGTGACATTTGGAG
AGGCTCTGTGGGAGCCCAGCCTGATCTTTGCTTTAGCCCACTTTGATGGGATCCTGGGCCTCGGCTTCCC
CACTCTGGCTGTGGGCGGAGTTCAGCCTCCGCTGGATGCGATGGTGGAGCAAGGGCTGCTGGAGAAACCC
GTCTTCTCCTTTTACCTCAACAGGGATTCTGAAGGGTCTGATGGGGGAGAGCTGGTCCTAGGGGGCTCAG
ACCCCGCTCACTACGTACCTCCCCTCACCTTCATACCAGTCACCATCCCTGCCTACTGGCAGGTCCACAT
GGAGAGTGTGAAGGTCGGCACAGGGCTGAGCCTCTGTGCCCAGGGCTGCAGTGCCATCCTAGACACAGGC
ACATCCCTCATCACAGGACCTAGTGAGGAGATCCGGGCCTTGAATAAAGCCATTGGGGGATATCCCTTCC
TGAATGGGCAGTACTTCATTCAGTGTTCCAAGACGCCAACGCTTCCCCCTGTCTCCTTCCACCTTGGTGG
AGTCTGGTTTAACCTCACAGGCCAGGACTATGTCATCAAGATTCTTCAGAGCGATGTTGGCCTCTGCCTG
TTGGGCTTCCAAGCCTTGGATATCCCCAAGCCTGCGGGACCCCTCTGGATCCTTGGGGACGTCTTTTTGG
GGCCCTATGTGGCTGTCTTTGACCGTGGGGACAAAAACGTCGGCCCGCGCGTGGGACTGGCGCGTGCTCA
GTCTCGTTCAACAGACCGGGCAGAAAGAAGGACTACGCAGGCGCAGTTCTTCAAAAGACGCCCTGGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206657 protein sequence
Red=Cloning site Green=Tags(s)

MSPLLLLLLCLLLGNLEPEEAKLIRVPLQRIHLGHRILNPLNGWEQLAELSRTSTSGGNPSFVPLSKFMN
TQYFGTIGLGTPPQNFTVVFDTGSSNLWVPSTRCHFFSLACWFHHRFNPKASSSFRPNGTKFAIQYGTGR
LSGILSQDNLTIGGIHDAFVTFGEALWEPSLIFALAHFDGILGLGFPTLAVGGVQPPLDAMVEQGLLEKP
VFSFYLNRDSEGSDGGELVLGGSDPAHYVPPLTFIPVTIPAYWQVHMESVKVGTGLSLCAQGCSAILDTG
TSLITGPSEEIRALNKAIGGYPFLNGQYFIQCSKTPTLPPVSFHLGGVWFNLTGQDYVIKILQSDVGLCL
LGFQALDIPKPAGPLWILGDVFLGPYVAVFDRGDKNVGPRVGLARAQSRSTDRAERRTTQAQFFKRRPG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008437
ORF Size 1257 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008437.1, NP_032463.1
RefSeq Size 1479 bp
RefSeq ORF 1260 bp
Locus ID 16541
UniProt ID O09043
Cytogenetics 7 B3
MW 45.5 kDa
Summary May be involved in processing of pneumocyte surfactant precursors.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008437" in other vectors (4)
SKU Description Size Price
MC204501 Napsa (untagged) - Mouse napsin A aspartic peptidase (Napsa), (10ug) 10 ug
$457.00
MG206657 Napsa (tGFP-tagged) - Mouse napsin A aspartic peptidase (Napsa) 10 ug
$886.00
MR206657L3 Lenti ORF clone of Napsa (Myc-DDK-tagged) - Mouse napsin A aspartic peptidase (Napsa) 10 ug
$986.00
MR206657L4 Lenti ORF clone of Napsa (mGFP-tagged) - Mouse napsin A aspartic peptidase (Napsa) 10 ug
$986.00

Plasmid Map for MR206657

Download sequence for

Cloning scheme for MR206657

Circular map for MR206657

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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