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Elmo1 (BC031782) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206637
Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Other products for "Elmo1 or BC031782"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Elmo1
Synonyms CED-12, 6330578D22Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206637 representing BC031782
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGACTAAGATGGACCCCCAGGACCAGGCCCAAAGGGACATCATATTTGAACTTCGAAGAATTGCTT
TCGATGCAGAGTCAGAACCTAATAACAGCAGTGGCAGCATGGAGAAACGCAAGTCCATGTACACTCGGGA
TTATAAAAAACTTGGCTTCATTAATCACGTCAATCCTGCCATGGACTTCACACAGACCCCTCCTGGGATG
TTGGCTCTGGACAACATGCTGTATTTCGCTAAGCACCACCAGGATGCATACATCCGGATCGTGCTGGAGA
ACAGCAGCCGAGAAGATAAACATGAGTGCCCCTTCGGCCGCAGCAGTATAGAGCTGACCAAGATGCTGTG
TGAGATCCTGAAAGTGGGCGAGCTGCCTAGTGAGACCTGCAACGACTTTCACCCGATGTTCTTCACCCAT
GACAGATCTTTTGAGGAATTCTTCTGCATTTGCATTCAACTCCTGAACAAAACATGGAAGGAAATGAGGG
CAACATCTGAAGACTTCAACAAGGTAATGCAGGTGGTGAAGGAGCAGGTTATGAGAGCGCTTACAACCAA
GCCTAGTTCCCTGGACCAGTTCAAAAGCAAGCTGCAAAACCTGAGCTACACCGAGATCCTGAAAATCCGC
CAGTCTGAGAGGATGAACCAGGAAGATTTCCAGTCTCGCCCGATTTTGGAACTAAAGGAGAAGATCCAGC
CAGAAATCTTAGAGCTGATTAAACAGCAGCGCCTGAACCGCCTTGTGGAAGGGACCTGCTTTAGGAAACT
CAATGCTCGCCGGAGACAAGACAAGTTTTGGTATTGTCGGCTTTCACCAAATCACAAGGTCTTACATTAT
GGTGACTTGGAAGAGAGCCCCCAAGGAGAAGTGCCCCACGATTCCCTGCAGGACAAATTGCCGGTGGCAG
ATATCAAAGCAGTGGTGACGGGAAAGGACTGCCCTCATATGAAAGAGAAAGGTGCCCTTAAACAAAACAA
GGAGGTGCTTGAACTTGCTTTCTCCATCTTATACGACTCAAATTGCCAACTGAACTTCATTGCTCCTGAT
AAGCATGAGTACTGCATCTGGACAGATGGGCTGAATGCACTGCTTGGGAAGGACATGATGAGTGACCTGA
CACGCAATGACCTGGACACCCTGCTGAGCATGGAGATCAAGCTTCGCCTGCTGGACCTGGAAAACATCCA
GATCCCGGATGCACCTCCGCCTATCCCCAAGGAACCTAGCAACTATGACTTTGTCTATGACTGTAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206637 representing BC031782
Red=Cloning site Green=Tags(s)

MMTKMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSMEKRKSMYTRDYKKLGFINHVNPAMDFTQTPPGM
LALDNMLYFAKHHQDAYIRIVLENSSREDKHECPFGRSSIELTKMLCEILKVGELPSETCNDFHPMFFTH
DRSFEEFFCICIQLLNKTWKEMRATSEDFNKVMQVVKEQVMRALTTKPSSLDQFKSKLQNLSYTEILKIR
QSERMNQEDFQSRPILELKEKIQPEILELIKQQRLNRLVEGTCFRKLNARRRQDKFWYCRLSPNHKVLHY
GDLEESPQGEVPHDSLQDKLPVADIKAVVTGKDCPHMKEKGALKQNKEVLELAFSILYDSNCQLNFIAPD
KHEYCIWTDGLNALLGKDMMSDLTRNDLDTLLSMEIKLRLLDLENIQIPDAPPPIPKEPSNYDFVYDCN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC031782
ORF Size 1257 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC031782, AAH31782
RefSeq Size 2540 bp
RefSeq ORF 1259 bp
Locus ID 140580
Cytogenetics 13 A2
MW 93.1 kDa
Summary Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).UniProtKB/Swiss-Prot Function
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"BC031782" in other vectors (4)
SKU Description Size Price
MC217958 Elmo1 (untagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C, (10ug) 10 ug
$503.00
MG206637 Elmo1 (tGFP-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C 10 ug
$489.00 $657.00
MR206637L3 Lenti ORF clone of Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C 10 ug
$757.00
MR206637L4 Lenti ORF clone of Elmo1 (mGFP-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C 10 ug
$757.00

Plasmid Map for MR206637

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206637
Circular map for MR206637

file-alt Citations

Cross-talk between miR-471-5p and autophagy component proteins regulates LC3-associated phagocytosis (LAP) of apoptotic germ cells
Panneerdoss, Viswanadhapalli, Abdelfattah et al
Nat Commun (2017) 8 (1), 598 DOI: 10.1038/s41467-017-00590-9
AI Summary: OriGene products contributed to this study by providing MAP1LC3B (cat #MG200654) and ELMO1 (cat #MR206637) plasmids, which were used to investigate the molecular mechanisms underlying LC3-associated phagocytosis in Sertoli cells.
Snippet: MAP1LC3B and ELMO1 plasmids were purchased from Origene (MAP1LC3B;cat #MG200654;ELMO1 cat #MR206637)
FigureFigureFigure
Showing 1-1 of 1 papers.
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