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Pdk3 (NM_145630) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206565
Pdk3 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdk3
Synonyms 2610001M10Rik; AI035637
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206565 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCTCTTCTACCGGCTGCTCAAGCAGCCGGTCCCCAAGCAGATCGAGCGCTACTCCCGCTTCTCCC
CGTCGCCACTGTCTATCAAACAGTTCCTGGACTTCGGAAGGGATAATGCATGTGAAAAAACTTCCTATAT
GTTTCTGCGCAAGGAACTTCCTGTGCGACTGGCTAACACCATGAGAGAGGTTAATCTCTTGCCGGATAAC
TTGTTGAACCGCCCTTCCGTGGGATTAGTTCAGAGCTGGTACATGCAGAGCTTCCTTGAACTTTTAGAAT
ATGAAAACAAGAGCCCAGAAGACCCACGAGTTTTGGATAACTTTCTCAACGTTCTGATAAATATCAGAAA
CAGACACAATGATGTTGTTCCTACAATGGCCCAAGGCGTGATTGAGTACAAGGAAAAGTTCGGGTTTGAT
CCGTTCATTAGCAGTAACATTCAATATTTCCTGGATCGGTTTTATACCAACCGCATTTCTTTCCGCATGC
TTATTAACCAGCACACACTTCTGTTTGGTGGTGACACTAACCCTGCACATCCGAAACATATAGGGAGTAT
CGACCCCACCTGTAATGTAGCTGATGTGGTTAAAGATGCATATGAAACAGCCAAGATGCTTTGTGAACAG
TATTACCTGGTAGCTCCAGAGCTAGAAGTTGAAGAATTCAATGCCAAAGCGCCAAACAAACCCATTCAGG
TAGTTTATGTGCCGTCACATCTGTTTCACATGCTATTTGAGCTGTTCAAGAACTCAATGAGGGCAACAGT
TGAACTACATGAAGATAAAAAAGAAGGCTACCCAGCTGTTAAAACTCTCGTTACTCTGGGTAAAGAAGAC
TTGTCCATTAAGATAAGTGACCTAGGTGGTGGAGTCCCACTTCGAAAAATAGACCGTCTTTTTAACTACA
TGTACTCAACTGCTCCTCGTCCCAGCCTGGAGCCTACAAGAGCGGCGCCCTTGGCTGGATTTGGTTATGG
CTTGCCAATTTCTCGTCTGTATGCCAGATATTTTCAGGGAGATCTAAAACTGTATTCCATGGAAGGAGTG
GGTACCGATGCAGTCATTTATTTGAAGGCCCTTTCAAGTGAATCCTTTGAGAGGCTGCCAGTTTTCAATA
AGTCTGCATGGCGCCATTACAAGACCACTCCTGAAGCTGATGACTGGAGCAATCCCAGCAGTGAACCAAG
GGATGCATCAAAATACAAGGCTAAACAGGACAAGATCAAGAGTAATAGAACTTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206565 protein sequence
Red=Cloning site Green=Tags(s)

MRLFYRLLKQPVPKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDN
LLNRPSVGLVQSWYMQSFLELLEYENKSPEDPRVLDNFLNVLINIRNRHNDVVPTMAQGVIEYKEKFGFD
PFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCNVADVVKDAYETAKMLCEQ
YYLVAPELEVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNSMRATVELHEDKKEGYPAVKTLVTLGKED
LSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGV
GTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNPSSEPRDASKYKAKQDKIKSNRTF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145630
ORF Size 1245 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145630.3
RefSeq Size 2000 bp
RefSeq ORF 1248 bp
Locus ID 236900
UniProt ID Q922H2
Cytogenetics X C3
MW 47.9 kDa
Summary Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species (By similarity).UniProtKB/Swiss-Prot Function
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"NM_145630" in other vectors (4)
SKU Description Size Price
MC200607 Pdk3 (untagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$457.00
MG206565 Pdk3 (tGFP-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3) 10 ug
$657.00
MR206565L3 Lenti ORF clone of Pdk3 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR206565L4 Lenti ORF clone of Pdk3 (mGFP-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR206565

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206565
Circular map for MR206565

file-alt Citations

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