OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Wdr4 (BC039272) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR206503
Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Wdr4 or BC039272"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wdr4
Synonyms AI415180; AI448349; D530049K22Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206503 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAGCTCTGCGGGGCTGGCACTGTGCGCCCAGACGCTGGTGGTGCGAGGAGGCAGCCGGTTCCTAG
CCTTCTCCACTACGGGCAGTGATGATGACTGTGTCTTCACATACGACTGCAGTACTGCAGAGAAGAAGGC
CACGCCAGAAGATAAAGGGGAGGACGGACAGCCCGCAGACACAGGGAGTGACTCGATTCTGGCGTCCACC
TTCTCCAAGTCTGGCCGCTATTTTGCTTTAACAGATGACAGTAAGCGTCTGATTCTTTTCCGTACAAAAC
CATGGCAATGTCTGAGTGTCAGGATGGTGGTGCGGAGGTGCACCGCCCTGACCTTCACAGCCTCAGAGGA
CCGAGTCTTGGTGGCTGACAAGTCTGGAGACGTCTACTCCTTTTCGGTGCTGGAGCCAGATGGATGTGGC
AGGCTGGAGCTTGGGCACCTCTCCATGCTGCTAGACGTGGCTGTGAGTCCTGATGACCAGTTTGTGCTTA
CTGCAGACCGGGATGAGAAGATCCGGGTCAGCTGGGCTGCTGCCCCGCATAGCATCGAGTCTTTCTGCCT
GGGACACACTGAGTTTGTGAGCCGCATCCTTGTAGTGCCCAGTCATCCTGAACTGCTGCTTTCTTCCTCT
GGGGATGGCACCCTGAGACTCTGGGAGTACAGAAGCGGTAGGCAGCTGCAGTGCTGTGACCTGGCCGGCC
TACAGGAGCCTGGAGAGCAGCCAGGCCACAAGGGGTTGGCCGCGTCCAGGATTGCATTCTGGGGACAGGA
GAGCTATGTGGTGCTTCTGTGTGAGTGCGTTCCCGTGGTCTTCGTCTTCCAGCTTGATGCCAGCAGACAG
CAGCTGGTGTTCAGACAGCGGCTGACTTTCCCTCACCGAGTGTGGGATGTTGTGTTTGAGGAGGCCCGGG
GGCTGTGGGTTCTACAGGACTGCCGTGATGCCCCCCTGGTGCTCTGGAGGCCTGTGGGTGGTGAGTGGCA
GGCTGCTCCAGACGGTGCTGTGTCCCCGAGACTCTGCAGCCATCTCCGTGAGAGCTGGGCCATGCTGGAA
GGTTCTGTTGGTACAGATGACAGCTTCCGCAGCCTGTACAAGGCCACCTTTGACAACATGACCTCTTACC
TGAAGAAAAAGGAAGAGAGACTGCAGCAGCAGCTGAAGAAGAAGCGGCAAAGGAGCCCCTTCCCAGGGTC
CCCGGAACAGACCAAAAAGGCGTGCCCGGGCCAGTCAGCCCTTAGTTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206503 protein sequence
Red=Cloning site Green=Tags(s)

MASSAGLALCAQTLVVRGGSRFLAFSTTGSDDDCVFTYDCSTAEKKATPEDKGEDGQPADTGSDSILAST
FSKSGRYFALTDDSKRLILFRTKPWQCLSVRMVVRRCTALTFTASEDRVLVADKSGDVYSFSVLEPDGCG
RLELGHLSMLLDVAVSPDDQFVLTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRILVVPSHPELLLSSS
GDGTLRLWEYRSGRQLQCCDLAGLQEPGEQPGHKGLAASRIAFWGQESYVVLLCECVPVVFVFQLDASRQ
QLVFRQRLTFPHRVWDVVFEEARGLWVLQDCRDAPLVLWRPVGGEWQAAPDGAVSPRLCSHLRESWAMLE
GSVGTDDSFRSLYKATFDNMTSYLKKKEERLQQQLKKKRQRSPFPGSPEQTKKACPGQSALSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC039272
ORF Size 1239 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC039272, AAH39272
RefSeq Size 2054 bp
RefSeq ORF 1241 bp
Locus ID 57773
Cytogenetics 17 B1
MW 45.8 kDa
Summary Non-catalytic component of a methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320). In the methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit (By similarity). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (PubMed:29983320). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (By similarity). Also required for methylation of a specific subset of miRNAs, such as let-7 (By similarity). Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320). Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed:29574139).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"BC039272" in other vectors (3)
SKU Description Size Price
MG206503 Wdr4 (tGFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600) 10 ug
$657.00
MR206503L3 Lenti ORF clone of Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600) 10 ug
$757.00
MR206503L4 Lenti ORF clone of Wdr4 (mGFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600) 10 ug
$757.00

Plasmid Map for MR206503

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206503
Circular map for MR206503

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products