Mterf3 (NM_025547) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206485
Mterfd1 (Myc-DDK-tagged) - Mouse MTERF domain containing 1 (Mterfd1), nuclear gene encoding mitochondrial protein
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mterf3
Synonyms 2410017I18Rik; Mterfd1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206485 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGTTAGCCCAACAGTTACCCAGACGGTTCAACTCAGTTAAGTTGACTAGCTTCATTAAAGCCA
AACAAGTCACCAAACGCTCTGCAAGAGCAGGAAAAGCCGTGTTGCCCGGCTTCTCAGCTCAGCCTCTGCT
CTCCTCTGACACGAGCTTTCTCCGGCGGGGAATTAAGACTTACAGGACTTTGTTCTGGAATCGTTTCCAT
TCTGCCAGCACAAATAGGACAAAGAGTTCTGCCGAAAGCACTCTGCTTCCTTCAGTGGCTGAACAGCAGG
AGAGGATACTTAGTCTTGAATCAGAGCTGCCTCTTGAAGAAGTGGATGACCTGCCTCCATTGTCTCCGTT
ACAGTCAGTTTCAGAGGAGGAGGCTATTCAGATTGCTGCGTACTCTCCGCTGCCCATCTCCTCCTTCACA
CTTGCAGATTACGTAGACCATTCCAAGACTCTGCAGAAGCTAGTGCAGCTTGGAGTGGATTTGTCTAAGA
TAGAAAAACATCCAGATGCAGCCAACCTCCTTCTAAGGCTGGATTTTGAAAAGCACATTAAGCAAATACT
GCTATTTCTTAAAGATTTGGGTTTAGAAGATAACCAGCTGGGACCATTTCTGACAAAAAATTATGCTATT
TTCTCTGAAGACCTTGAAAACCTTAAGACCAGAGTAGCTTATCTACAGTCAAAAAATTTCAGTAAGACAG
ACATTGCACGGATGGTCAAAAATGCACCATTTCTGTTGAGCTTCTCCGTGGAAAGACTAGATAACAGACT
GGGATTTTTTCAGAAAGAACTTGAACTGAATGTGAAGAAGACTAGAGATCTGGTTGTTCGCCTTCCAAGG
CTACTAACAGGAAGTCTGGAGCCTGTGAAGGAAAACATGAAGGTTTATCATCTTGAACTTGGTTTTAAAC
ATAATGAAATTCAACATATGGTCATCAAGATTCCTAAGATGCTAACTGCAAACAAAAGAAAACTTACAGA
GATTTTTGATTATGTACACAATGTGATGAATATTCCCCACCACATCATCGTCAAGTTTCCACAGTTATTT
AACACAAGAGTGTTTAAAATTAAAGAAAGACACTTGTTTCTTGCCTACCTGGGAAGAGCACAGTATGACC
CAGCAAAACCTAACTATGTTTCTTTGGACAAGTTCGTATCTTTTCCTGATAAAATATTTTGTAAAGAGAT
TGCCAAAGCTTCACTAAATGATTTTGAGAAATTCTTAAAAACGCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206485 protein sequence
Red=Cloning site Green=Tags(s)

MALLAQQLPRRFNSVKLTSFIKAKQVTKRSARAGKAVLPGFSAQPLLSSDTSFLRRGIKTYRTLFWNRFH
SASTNRTKSSAESTLLPSVAEQQERILSLESELPLEEVDDLPPLSPLQSVSEEEAIQIAAYSPLPISSFT
LADYVDHSKTLQKLVQLGVDLSKIEKHPDAANLLLRLDFEKHIKQILLFLKDLGLEDNQLGPFLTKNYAI
FSEDLENLKTRVAYLQSKNFSKTDIARMVKNAPFLLSFSVERLDNRLGFFQKELELNVKKTRDLVVRLPR
LLTGSLEPVKENMKVYHLELGFKHNEIQHMVIKIPKMLTANKRKLTEIFDYVHNVMNIPHHIIVKFPQLF
NTRVFKIKERHLFLAYLGRAQYDPAKPNYVSLDKFVSFPDKIFCKEIAKASLNDFEKFLKTL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025547
ORF Size 1236 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025547.4
RefSeq Size 1486 bp
RefSeq ORF 1239 bp
Locus ID 66410
UniProt ID Q8R3J4
Cytogenetics 13 B3
MW 47.4 kDa
Summary Binds promoter DNA and regulates initiation of transcription (By similarity). Required for normal mitochondrial transcription and translation, and for normal assembly of mitochondrial respiratory complexes (PubMed:17662942, PubMed:23300484). Required for normal mitochondrial function (PubMed:17662942, PubMed:23300484). Maintains 16S rRNA levels and functions in mitochondrial ribosome assembly by regulating the biogenesis of the 39S ribosomal subunit (PubMed:17662942, PubMed:23300484).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025547" in other vectors (4)
SKU Description Size Price
MC203189 Mterfd1 (untagged) - Mouse MTERF domain containing 1 (Mterfd1), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$457.00
MG206485 Mterfd1 (tGFP-tagged) - Mouse MTERF domain containing 1 (Mterfd1) 10 ug
$657.00
MR206485L3 Lenti ORF clone of Mterfd1 (Myc-DDK-tagged) - Mouse MTERF domain containing 1 (Mterfd1), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR206485L4 Lenti ORF clone of Mterfd1 (mGFP-tagged) - Mouse MTERF domain containing 1 (Mterfd1), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR206485

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Cloning scheme for MR206485

Circular map for MR206485

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