Pafah1b1 (BC026141) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR206439
Pafah1b1 (Myc-DDK-tagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit (cDNA clone MGC:13913 IMAGE:4017963)
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Pafah1b1 |
| Synonyms | Lis1, LIS-1, MGC25297, MMS10-U, Ms10u |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | BC026141 |
| ORF Size | 1230 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC026141, AAH26141 |
| RefSeq Size | 1935 bp |
| RefSeq ORF | 1232 bp |
| Locus ID | 18472 |
| Cytogenetics | 11 45.76 cM |
| MW | 46.7 kDa |
| Summary | Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position. Required for dynein recruitment to microtubule plus ends and BICD2-bound cargos (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"BC026141" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG206439 | Pafah1b1 (tGFP-tagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit (cDNA clone MGC:13913 IMAGE:4017963) | 10 ug |
$657.00
|
|
| MR206439L3 | Lenti ORF clone of Pafah1b1 (Myc-DDK-tagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit (cDNA clone MGC:13913 IMAGE:4017963) | 10 ug |
$757.00
|
|
| MR206439L4 | Lenti ORF clone of Pafah1b1 (mGFP-tagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit (cDNA clone MGC:13913 IMAGE:4017963) | 10 ug |
$757.00
|
|
Citations
NuMA1 promotes axon initial segment assembly through inhibition of endocytosis
Torii, Ogawa, Liu et al
J. Cell Biol. (2019) 219 (2) DOI: 10.1083/jcb.201907048
Torii, Ogawa, Liu et al
J. Cell Biol. (2019) 219 (2) DOI: 10.1083/jcb.201907048
AI Summary: In this study, OriGene products including the Pafah1b1-myc-FLAG (Cat# BC026141), mouse NDE1-myc-FLAG (Cat# MR228536), and mouse DCX-myc-FLAG (Cat# MR205614) expression constructs were used to investigate protein interactions and mechanisms underlying axon initial segment assembly.
Snippet: Skip to Main Content Advertisement;(also known as Pafah1b1)-myc-FLAG (Cat# BC026141), mouse NDE1-myc-FLAG (Cat# MR228536), and mouse DCX-myc-FLAG (Cat# MR205614) constructs were purchased from Origene. Lis1-myc-FLAG was inserted into the pEGFP-N1 vector to create pEGFPN1-Lis1. Phosphomimetic mutants of NuMA1 (NuMA1 C-ter 1TD, NuMA1 C-ter 2TD, and NuMA1 C-ter 3T/SD)
Showing 1-1 of 1 papers.
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