Acy1 (NM_025371) Mouse Tagged ORF Clone
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Acy1 |
| Synonyms | 1110014J22Rik; Acy-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_025371 |
| ORF Size | 1224 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_025371.1, NM_025371.2, NM_025371.3, NP_079647.1 |
| RefSeq Size | 1459 bp |
| RefSeq ORF | 1227 bp |
| Locus ID | 109652 |
| UniProt ID | Q99JW2 |
| Cytogenetics | 9 57.49 cM |
| MW | 45.8 kDa |
| Summary | Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_025371" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC200771 | Acy1 (untagged) - Mouse aminoacylase 1 (Acy1), (10ug) | 10 ug |
$457.00
|
|
| MG206415 | Acy1 (tGFP-tagged) - Mouse aminoacylase 1 (Acy1) | 10 ug |
$657.00
|
|
| MR206415L3 | Lenti ORF clone of Acy1 (Myc-DDK-tagged) - Mouse aminoacylase 1 (Acy1) | 10 ug |
$757.00
|
|
| MR206415L4 | Lenti ORF clone of Acy1 (mGFP-tagged) - Mouse aminoacylase 1 (Acy1) | 10 ug |
$757.00
|
|
Citations
Proteomic profiling reveals biomarkers and pathways in type 2 diabetes risk
Ngo, Benson, Long et al
JCI Insight (2021) 6 (5) DOI: 10.1172/jci.insight.144392
Ngo, Benson, Long et al
JCI Insight (2021) 6 (5) DOI: 10.1172/jci.insight.144392
Cited by 85
AI Summary: OriGene’s pCMV6-ENTRY plasmid containing the full-length mouse ACY1 gene (NM_025371) was used to generate AAV vectors for functional studies investigating the role of ACY1 in type 2 diabetes risk.
Snippet: AAV production. pCMV6-ENTRY plasmid containing a full-length mouse ACY-1-myc-ddk insert was purchased (NM_025371, OriGene Technologies) and subcloned into pENN.AAV8.CB7.CI.WPRE.rBG (Penn Vector Core, Philadelphia, Pennsylvania, USA) using the EcoRV/XhoI sites
Cooperative enzymatic control of N-acyl amino acids by PM20D1 and FAAH
Kim, Terrell, Li et al
Elife (2020) 9 DOI: 10.7554/eLife.55211
Kim, Terrell, Li et al
Elife (2020) 9 DOI: 10.7554/eLife.55211
AI Summary: OriGene products—specifically FAAH-flag (MR209084), ACY1-flag (MR206415), CNDP1-flag (MR219018), and CNDP2-flag (MR207616) transfected constructs—were used to express and study the roles of these enzymes in the cooperative enzymatic regulation of N-acyl amino acids in this research.
Snippet: Mouse line (M. musculus) C57BL/6J Jackson Labs 000664 Transfected construct (M. musculus) PM20D1-flag Addgene 84566 Transfected construct (M. musculus) FAAH-flag Origene MR209084 Transfected construct (M. musculus) ACY1-flag Origene MR206415 Transfected construct (M. musculus) CNDP1-flag Origene MR219018 Transfected construct (M. musculus) CNDP2-flag Origene MR207616
Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo Metabolomics
Kim, Li, Terrell et al
Cell Chem Biol (2019) 26 (11), 1623-1629.e3 DOI: 10.1016/j.chembiol.2019.09.009
Kim, Li, Terrell et al
Cell Chem Biol (2019) 26 (11), 1623-1629.e3 DOI: 10.1016/j.chembiol.2019.09.009
AI Summary: OriGene products, specifically the mACY1 cDNA (MR206415), mPM20D2 cDNA (MR222068), mCNDP2 cDNA (MR207616), and mCNDP1 cDNA constructs, were used as sources of full-length mouse genes for generating adeno-associated viruses to study enzymatic pathways in vivo.
Snippet: …Full-length mouse _Pm20d1_, _Pm20d2_, _Cndp1, Cndp2_, and _Acy1_ cDNA (Origene) with C-terminal 6xHIS and Flag tags were subcloned into pENN.AAV8.CB7.CI.WPRE.rBG (Penn Vector Core) using the PstI/HindIII sites to generate AAV8 plasmids. … mACY1 cDNA, C-term myc-DDK tagged, Origene, MR206415. mPM20D2 cDNA, C-term myc-DDK tagged, Origene, MR222068. mCNDP2 cDNA, C-term myc-DDK tagged, Origene, MR207616. …
Showing 1-3 of 3 papers.
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