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Ccne2 (NM_001037134) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206360
Ccne2 (Myc-DDK-tagged) - Mouse cyclin E2 (Ccne2), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccne2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206360 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAAGACGCAGCCGTTTACAAGCTAAGCAACATGCCCAGCCCAACCAGCCAGACTCTCCGCAAGAAA
CCCAGATAATTCAGGCCAAGAAGAGAAAAACAGCACAGGATGTCAAAAAAAGAAAAGAGGAGATCACCAA
GAAGCATCAGTATGAGATTAGGAATTGTTGGCCACCTGTACTGTCTGGAGGAATCAGCCCTTGCATTATC
ATTGAAACACCCCATAAAGAAATAGGAACAAGTGACTTCTCTAGATTTACAAATTACAGATTTAAAAATC
TTTTTATTAATCCCTCACCTCTGCCAGATTTAAGCTGGGCATGTTCACAGGAGGTTTGGCAAAACATGTT
ACAAAAGGAAAACAGATACGTGCATGACAAACATTTTCAAGTTCTGCATTCTGACCTGGAACCACAGATG
AGGTCAATACTTTTAGACTGGCTTTTAGAGGTTTGTGAAGTATACACTCTTCATAGGGAGACATTTTACC
TTGCCCAAGACTTTTTTGACAGATTTATGTTGACACAAAAGGATGTAAATAAAAATATGCCTCAACTCAT
TGGGATTACCTCATTGTTCATTGCTTCCAAACTTGAGGAAATCTACGCTCCTAAACTCCAAGAGTTTGCT
TACGTCACTGATGGTGCTTGCAGTGAAGTAGATATCTTAAAGGTGGAACTCAATATATTAAAGGCTTTAA
AATGGGAACTTTGTCCAGTAACAGTCATCTCCTGGTTGAATCTTTTTCTTCAAGTTGATGCTGTTAAAGA
TGTTCCTAAGGTTCTTCTGCCTCAATATTCTCAGGAGACGTTCATCCAGATAGCTCAGCTTTTAGATCTG
TGCATTCTAGCCATCGACTCTTTAGAATTTCAATACAGAATTCTGGTTGCTGCCGCCTTATGTCATTTTA
CCTCCATTGAAGTGGTTAAGAAAGCTTCAGGTTTGGAATGGGATGACATCTCGGAATGTGTAGACTGGAT
GGTGCCTTTTGTTAGTGTTGTAAAAAGTGTGAGTCCAGTGAAGCTGAAGACTTTTAAGAAGATACCCATG
GAAGATAGACACAATATCCAGACACACACAAATTATTTGGCTTTGCTGAATGAAGTAAACTATGTGAACA
TCTACAGAAAAGGAGGGCAGCTGTCACCAGTGTGTAATGGAGGCATTATGACACCACCAAAGAGTACTGA
AAAACCACCAGGAAAACAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206360 protein sequence
Red=Cloning site Green=Tags(s)

MSRRSRLQAKQHAQPNQPDSPQETQIIQAKKRKTAQDVKKRKEEITKKHQYEIRNCWPPVLSGGISPCII
IETPHKEIGTSDFSRFTNYRFKNLFINPSPLPDLSWACSQEVWQNMLQKENRYVHDKHFQVLHSDLEPQM
RSILLDWLLEVCEVYTLHRETFYLAQDFFDRFMLTQKDVNKNMPQLIGITSLFIASKLEEIYAPKLQEFA
YVTDGACSEVDILKVELNILKALKWELCPVTVISWLNLFLQVDAVKDVPKVLLPQYSQETFIQIAQLLDL
CILAIDSLEFQYRILVAAALCHFTSIEVVKKASGLEWDDISECVDWMVPFVSVVKSVSPVKLKTFKKIPM
EDRHNIQTHTNYLALLNEVNYVNIYRKGGQLSPVCNGGIMTPPKSTEKPPGKH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001037134
ORF Size 1209 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001037134.1, NM_001037134.2, NP_001032211.1
RefSeq Size 3113 bp
RefSeq ORF 1215 bp
Locus ID 12448
UniProt ID Q9Z238
Cytogenetics 4 A1
MW 46.6 kDa
Summary Essential for the control of the cell cycle at the late G1 and early S phase.UniProtKB/Swiss-Prot Function
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Documents
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"NM_001037134" in other vectors (4)
SKU Description Size Price
MC202066 Ccne2 (untagged) - Mouse cyclin E2 (Ccne2), transcript variant 1, (10ug) 10 ug
$457.00
MG206360 Ccne2 (tGFP-tagged) - Mouse cyclin E2 (Ccne2), transcript variant 1 10 ug
$657.00
MR206360L3 Lenti ORF clone of Ccne2 (Myc-DDK-tagged) - Mouse cyclin E2 (Ccne2), transcript variant 1 10 ug
$757.00
MR206360L4 Lenti ORF clone of Ccne2 (mGFP-tagged) - Mouse cyclin E2 (Ccne2), transcript variant 1 10 ug
$757.00

Plasmid Map for MR206360

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206360
Circular map for MR206360

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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