Fbxl14 (NM_133940) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206290
Fbxl14 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 14 (Fbxl14)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbxl14
Synonyms AW322056; Fbx14l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206290 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACCCACATCTCGTGCCTGTTCCCCGAGCTGCTGGCCATGATCTTCGGCTACTTGGACTTCCGAG
ACAAGGGTCGCGCGGCGCAGGTGTGCACGGCCTGGCGGGACGCCGCCTACCACAAGTCGGTGTGGCGGGG
CGTGGAGGCCAAGCTGCACCTGCGCCGGGCGAACCCGTCGCTGTTCCCCAGCCTGCAGGCCCGGGGCATT
CGCCGCGTGCAGATCCTCAGCCTCCGCCGCAGCCTCAGCTACGTGATCCAGGGCATGGCGAACATCGAGA
GCCTCAACCTCAGCGGCTGCTACAACCTCACCGACAACGGCCTGGGCCACGCGTTCGTGCAGGAGATCGG
CTCGCTGCGCGCTCTCAACTTGAGCCTTTGCAAGCAGATCACCGACAGCAGCCTGGGCCGCATAGCCCAG
TACCTCAAGGGCCTGGAGGTGCTGGAGCTGGGGGGCTGCAGCAACATCACCAACACCGGCCTTCTGCTCA
TTGCCTGGGGGCTGCAGCGCCTCAAGAGCCTTAATCTCCGCAGCTGTCGCCACCTCTCGGATGTGGGCAT
CGGGCACCTGGCCGGCATGACGCGCAGCGCTGCCGAGGGCTGCCTGGGCCTGGAGCAGCTCACTCTTCAG
GACTGCCAGAAACTCACGGATCTTTCCCTGAAGCACATCTCGCGAGGGCTGACGGGCCTCCGGCTCCTCA
ACCTCAGCTTCTGCGGCGGCATCTCGGACGCGGGCCTTCTGCACCTGTCGCACATGGGCAGCCTGCGCAG
CCTGAACCTGCGCTCCTGCGACAACATCAGCGACACCGGCATCATGCATCTGGCCATGGGCAGCCTGCGC
CTTTCCGGTCTGGATGTGTCGTTCTGTGACAAGGTGGGAGATCAGAGTCTGGCGTACATAGCCCAGGGAC
TGGACGGCCTCAAGTCCCTCTCCCTTTGCTCTTGCCATATCAGTGACGATGGCATCAACCGCATGGTGCG
GCAGATGCACGGGCTGCGCACGCTCAACATTGGACAGTGTGTGCGCATCACGGACAAGGGCCTGGAGCTG
ATCGCTGAGCACCTGAGCCAGCTCACGGGCATAGATCTGTACGGCTGCACCAGGATCACCAAGCGCGGCC
TGGAGCGCATCACGCAGCTGCCCTGCCTCAAGGTACTTAACCTGGGACTTTGGCAGATGACGGACAGTGA
GAAGGTCAGG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206290 protein sequence
Red=Cloning site Green=Tags(s)

METHISCLFPELLAMIFGYLDFRDKGRAAQVCTAWRDAAYHKSVWRGVEAKLHLRRANPSLFPSLQARGI
RRVQILSLRRSLSYVIQGMANIESLNLSGCYNLTDNGLGHAFVQEIGSLRALNLSLCKQITDSSLGRIAQ
YLKGLEVLELGGCSNITNTGLLLIAWGLQRLKSLNLRSCRHLSDVGIGHLAGMTRSAAEGCLGLEQLTLQ
DCQKLTDLSLKHISRGLTGLRLLNLSFCGGISDAGLLHLSHMGSLRSLNLRSCDNISDTGIMHLAMGSLR
LSGLDVSFCDKVGDQSLAYIAQGLDGLKSLSLCSCHISDDGINRMVRQMHGLRTLNIGQCVRITDKGLEL
IAEHLSQLTGIDLYGCTRITKRGLERITQLPCLKVLNLGLWQMTDSEKVR

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_133940
ORF Size 1200 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133940.2
RefSeq Size 4219 bp
RefSeq ORF 1203 bp
Locus ID 101358
UniProt ID Q8BID8
Cytogenetics 6 F1
MW 43.9 kDa
Summary Substrate-recognition component of some (SKP1-CUL1-F-box protein)-type E3 ubiquitin-protein ligase complexes. The SCF(FBXL14) complex acts by mediating ubiquitination and subsequent degradation of SNAI1 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133940" in other vectors (4)
SKU Description Size Price
MC205423 Fbxl14 (untagged) - Mouse F-box and leucine-rich repeat protein 14 (Fbxl14), (10ug) 10 ug
$457.00
MG206290 Fbxl14 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 14 (Fbxl14) 10 ug
$657.00
MR206290L3 Lenti ORF clone of Fbxl14 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 14 (Fbxl14) 10 ug
$757.00
MR206290L4 Lenti ORF clone of Fbxl14 (mGFP-tagged) - Mouse F-box and leucine-rich repeat protein 14 (Fbxl14) 10 ug
$757.00

Plasmid Map for MR206290

Download sequence for

Cloning scheme for MR206290

Circular map for MR206290

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