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Klhl7 (NM_001161800) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206281
Klhl7 (Myc-DDK-tagged) - Mouse kelch-like 7 (Drosophila) (Klhl7), transcript variant 2
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Klhl7
Synonyms 2700038B03Rik; D5Ertd363e; SBBI26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206281 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCTCCGGCGTGGAAAAGAGTAGCAAGAAGAAAACTGAGAAGAAACTTGCGGCTCGCGAAGAAG
CCAAGCTGCTGGCGGGGTTCATGGGCGTCATGAACAACATGAGGAAACAGAGAACACTGTGTGACGTGAT
CCTCACCGTCCAGGAAAGAAAGATCCCCGCTCACCGCGTTGTCCTGGCTGCAGCCAGTCACTTCTTTAAC
TTAATGTTCACAACTAACATGCTTGAATCTAAGTCCTTTGAAGTAGAACTCAAAGATGCTGAACCTGATA
TTATTGAACAACTGGTGGAATTTGCTTATACTGCTAGAATTTCTGTGAATAGCAACAATGTTCAGTCTTT
GTTAGATGCAGCAAATCAGTACCAGATTGAACCTGTGAAGAAAATGTGTGTTGATTTTTTGAAAGAACAA
GTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCTTGCAGAGTGTCTGGACTGTCCTGAACTGAAAGCTA
CAGCTGACGACTTCATTCATCAGCATTTCACTGAGGTTTACAAAACAGATGAATTCCTACAGCTTGATGT
CAAGCGAGTGACACATCTTCTCAGCCAGGACACCCTGACTGTGAGAGCAGAGGACCAGGTTTATGACGCT
GCCGTGAGGTGGTTGAAATATGATGAGCCTAACCGCCAGCCATTCATGGTTGATATCCTTGCTAAAGTCA
GGTTTCCTCTTATATCGAAGAATTTCCTAAGTAAAACAGTACAAGCTGAACCACTTATTCAAGATAATCC
TGAATGCCTTAAAATGGTGATAAGTGGAATGAGGTACCATCTGCTGTCTCCTGAGGATCGAGAAGAACTT
GCAGGTGGCACACGGCCTAGAAGAAAGAAACATGATTACCGCATAGCTCTGTTTGGAGGCTCCCAACCAC
AGTCCTGTAGATATTTTAACCCAAAGGATTACAGTTGGACAGACATTCGCTGCCCCTTTGAAAAACGAAG
AGATGCAGCATGTGTGTTTTGGGACAATGTAGTATACATTTTGGGAGGTTCTCAGCTTTTCCCCATAAAG
CGAATGGATTGCTACAATGTTGTAAAGGATAGCTGGTATTCCAAACTGGGCCCTCCAACACCTCGAGACA
GCCTTGCTGCCTGTGCAGCAGAAGGCAAAAATTTATACATCTGGAGGTTCAGAAGTAGGAAACTCGGCCC
TGTATTTGTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206281 protein sequence
Red=Cloning site Green=Tags(s)

MATSGVEKSSKKKTEKKLAAREEAKLLAGFMGVMNNMRKQRTLCDVILTVQERKIPAHRVVLAAASHFFN
LMFTTNMLESKSFEVELKDAEPDIIEQLVEFAYTARISVNSNNVQSLLDAANQYQIEPVKKMCVDFLKEQ
VDASNCLGISVLAECLDCPELKATADDFIHQHFTEVYKTDEFLQLDVKRVTHLLSQDTLTVRAEDQVYDA
AVRWLKYDEPNRQPFMVDILAKVRFPLISKNFLSKTVQAEPLIQDNPECLKMVISGMRYHLLSPEDREEL
AGGTRPRRKKHDYRIALFGGSQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFPIK
RMDCYNVVKDSWYSKLGPPTPRDSLAACAAEGKNLYIWRFRSRKLGPVFV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001161800
ORF Size 1200 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 3329 bp
RefSeq ORF 1422 bp
Locus ID 52323
Cytogenetics 5 10.67 cM
MW 45.9 kDa
Summary Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex. The BCR(KLHL7) complex acts by mediating ubiquitination and subsequent degradation of substrate proteins. Probably mediates 'Lys-48'-linked ubiquitination (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_001161800" in other vectors (3)
SKU Description Size Price
MC217790 Klhl7 (untagged) - Mouse kelch-like 7 (Drosophila) (Klhl7), transcript variant 2, (10ug) 10 ug
$503.00
MR206281L3 Lenti ORF clone of Klhl7 (Myc-DDK-tagged) - Mouse kelch-like 7 (Drosophila) (Klhl7), transcript variant 2 10 ug
$757.00
MR206281L4 Lenti ORF clone of Klhl7 (mGFP-tagged) - Mouse kelch-like 7 (Drosophila) (Klhl7), transcript variant 2 10 ug
$757.00

Plasmid Map for MR206281

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206281
Circular map for MR206281

file-alt Citations

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