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Isgf3g (BC005435) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR206259L3
Lenti ORF clone of Isgf3g (Myc-DDK-tagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $757.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Isgf3g
Synonyms p48, Irf-9
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR206259).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC005435
ORF Size 1197 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC005435.1
RefSeq Size 2341 bp
RefSeq ORF 1199 bp
Locus ID 16391
Cytogenetics 14 28.19 cM
Summary Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.UniProtKB/Swiss-Prot Function
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"BC005435" in other vectors (4)
SKU Description Size Price
MC200578 Isgf3g (untagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete, (10ug) 10 ug
$457.00
MG206259 Isgf3g (tGFP-tagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete 10 ug
$657.00
MR206259 Isgf3g (Myc-DDK-tagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete 10 ug
$289.00 $457.00
MR206259L4 Lenti ORF clone of Isgf3g (mGFP-tagged) - Mouse interferon dependent positive acting transcription factor 3 gamma (cDNA clone MGC:5974 IMAGE:3601255), complete 10 ug
$757.00

Plasmid Map for MR206259L3

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Cloning scheme for MR206259L3
Circular map for MR206259L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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