OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Gnb5 (NM_010313) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR206192
Gnb5 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), beta 5 (Gnb5), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Gnb5 or NM_010313"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gnb5
Synonyms flr; Gbeta5; GBS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206192 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCGATCAGACCTTCCTGGTTAATGTGTTCGGCTCATGTGACAAATGCTTCAAACAAAGGGCCCTAA
GACCCGTTTTCAAGAAATCTCAACAACTCAACTACTGCTCCACGTGTGCAGAGATCATGGCAACCGATGG
GCTGCACGAGAACGAGACGCTGGCGTCGCTGAAGAGCGAGGCCGAGAGCCTCAAGGGCAAGCTAGAGGAG
GAGCGGGCAAAGCTGCATGACGTGGAGCTACACCAGGTGGCCGAGCGGGTGGAGGCCCTGGGCCAGTTTG
TCATGAAGACCAGAAGGACCCTCAAAGGCCACGGGAACAAAGTCCTGTGCATGGATTGGTGCAAAGACAA
GCGGAGAATCGTGAGCTCCTCACAGGATGGGAAGGTGATTGTCTGGGATTCCTTCACTACGAACAAGGAG
CACGCGGTCACCATGCCTTGCACCTGGGTGATGGCGTGTGCTTACGCGCCATCGGGATGCGCCATTGCTT
GTGGTGGTCTGGATAATAAGTGCTCTGTGTATCCACTGACATTCGACAAGAACGAGAACATGGCCGCCAA
GAAGAAGTCTGTTGCTATGCACACCAACTACCTGTCTGCCTGCAGCTTCACCAACTCTGACATGCAGATT
CTGACAGCCAGCGGGGATGGCACCTGCGCCCTGTGGGATGTGGAAAGTGGACAGCTGTTACAGAGCTTCC
ATGGGCACGGGGCTGACGTCCTCTGCTTGGACCTGGCCCCCTCGGAAACAGGAAACACCTTCGTGTCTGG
GGGTTGTGACAAGAAGGCCATGGTGTGGGACATGCGCTCTGGCCAGTGCGTGCAAGCCTTTGAAACTCAT
GAATCGGATGTCAACAGTGTTCGGTACTACCCCAGTGGGGATGCTTTTGCTTCGGGGTCGGATGATGCCA
CGTGTCGCCTCTATGACCTGAGGGCAGATAGAGAGGTCGCCATCTACTCCAAGGAGAGCATCATATTTGG
CGCCTCGAGTGTGGACTTCTCCCTCAGTGGTCGCCTGCTTTTTGCTGGGTACAATGACTATACCATCAAT
GTCTGGGATGTTCTCAAGGGCTCCCGGGTCTCCATCCTGTTTGGACATGAAAACCGCGTCAGCACTCTGC
GAGTGTCTCCTGATGGGACAGCATTCTGCTCGGGATCATGGGATCACACCCTAAGAGTTTGGGCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206192 protein sequence
Red=Cloning site Green=Tags(s)

MCDQTFLVNVFGSCDKCFKQRALRPVFKKSQQLNYCSTCAEIMATDGLHENETLASLKSEAESLKGKLEE
ERAKLHDVELHQVAERVEALGQFVMKTRRTLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKE
HAVTMPCTWVMACAYAPSGCAIACGGLDNKCSVYPLTFDKNENMAAKKKSVAMHTNYLSACSFTNSDMQI
LTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQAFETH
ESDVNSVRYYPSGDAFASGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTIN
VWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010313
ORF Size 1185 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010313.2
RefSeq Size 2249 bp
RefSeq ORF 1188 bp
Locus ID 14697
UniProt ID P62881
Cytogenetics 9 42.3 cM
MW 43.6 kDa
Summary Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (Probable). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D(2) dopamine receptors (By similarity). May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_010313" in other vectors (4)
SKU Description Size Price
MC201261 Gnb5 (untagged) - Mouse guanine nucleotide binding protein (G protein), beta 5 (Gnb5), transcript variant 1, (10ug) 10 ug
$457.00
MG206192 Gnb5 (tGFP-tagged) - Mouse guanine nucleotide binding protein, beta 5 (Gnb5), transcript variant 1 10 ug
$657.00
MR206192L3 Lenti ORF clone of Gnb5 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), beta 5 (Gnb5), transcript variant 1 10 ug
$757.00
MR206192L4 Lenti ORF clone of Gnb5 (mGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), beta 5 (Gnb5), transcript variant 1 10 ug
$757.00

Plasmid Map for MR206192

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206192
Circular map for MR206192

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products