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Sox11 (NM_009234) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206174
Sox11 (Myc-DDK-tagged) - Mouse SRY-box containing gene 11 (Sox11)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sox11
Synonyms 1110038H03Rik; 6230403H02Rik; AI836553; end1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206174 representing NM_009234
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCAGCAGGCCGAGAGCTCGGAAGCCGAGAGCAACCTGCCCCGGGACGCGCTGGACACCGAGGAGG
GCGAGTTCATGGCGTGCAGCCCGGTGGCCCTGGACGAGAGCGACCCGGACTGGTGCAAGACGGCGTCGGG
CCACATCAAACGGCCCATGAACGCCTTCATGGTGTGGTCCAAGATCGAGCGCAGGAAGATCATGGAGCAG
TCGCCCGACATGCACAACGCCGAGATCTCCAAGAGGCTGGGCAAGCGCTGGAAGATGCTGAAGGACAGCG
AGAAGATCCCGTTCATCAGGGAGGCGGAGCGCCTGCGCCTCAAGCACATGGCTGATTATCCCGACTACAA
GTACCGGCCGCGCAAAAAGCCCAAGACGGACCCAGCGGCCAAGCCCAGCGCGGGCCAGAGCCCCGACAAG
AGCGCGGCGGGCGCCAAGGCAGCCAAGGGCCCCGGCAAGAAGTGCGCCAAGCTCAAGGCGCCTGCGGGCA
AGGCGGGCGCGGGCAAGGCGGCGCAGCCGGGGGACTGCGCCGCGGGCAAGGCAGCCAAGTGCGTCTTCCT
GGACGACGACGATGAAGACGACGACGAAGATGACGAGCTGCAGCTACGGCCCAAGCCGGACGCTGACGAC
GACGACGACGAGCCCGCGCACTCGCACCTGCTGCCGCCGCCGACGCAGCAGCAACCCCCTCAGCTGCTGA
GGCGCTACAGCGTGGCCAAGGTCCCCGCCAGCCCCACGCTCAGCAGTGCCGCCGAGTCCCCCGAGGGCGC
GAGCCTGTACGACGAAGTGCGCGCGGGCGGCCGGCTCTACTACAGCTTCAAGAACATCACCAAGCAGCAG
CCTCCGCCCGCGCCTCCCGCGCTGTCGCCCGCGTCCTCCCGCTGCGTGTCCACCTCCTCATCCAGCGGCA
GCAGCAGCGGCAGCGGCGCCGAGGATGCAGACGACCTCATGTTCGACCTGAGCTTGAATTTCTCCCAGGG
CGCGCACAGCGCCTGCGAGCAGCCACTGGGCGCGGGAGCGGCGGGGAACCTGTCCCTGTCGCTGGTGGAT
AAGGACCTGGATTCCTTCAGCGAGGGCAGCCTGGGTTCCCACTTCGAGTTCCCCGACTACTGCACGCCGG
AGCTGAGCGAGATGATCGCGGGGGACTGGCTGGAGGCGAACTTCTCCGACCTGGTGTTCACGTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206174 representing NM_009234
Red=Cloning site Green=Tags(s)

MVQQAESSEAESNLPRDALDTEEGEFMACSPVALDESDPDWCKTASGHIKRPMNAFMVWSKIERRKIMEQ
SPDMHNAEISKRLGKRWKMLKDSEKIPFIREAERLRLKHMADYPDYKYRPRKKPKTDPAAKPSAGQSPDK
SAAGAKAAKGPGKKCAKLKAPAGKAGAGKAAQPGDCAAGKAAKCVFLDDDDEDDDEDDELQLRPKPDADD
DDDEPAHSHLLPPPTQQQPPQLLRRYSVAKVPASPTLSSAAESPEGASLYDEVRAGGRLYYSFKNITKQQ
PPPAPPALSPASSRCVSTSSSSGSSSGSGAEDADDLMFDLSLNFSQGAHSACEQPLGAGAAGNLSLSLVD
KDLDSFSEGSLGSHFEFPDYCTPELSEMIAGDWLEANFSDLVFTY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009234
ORF Size 1185 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009234.6
RefSeq Size 7497 bp
RefSeq ORF 1188 bp
Locus ID 20666
UniProt ID Q7M6Y2
Cytogenetics 12 A2
MW 43.1 kDa
Summary Transcriptional factor involved in the embryonic neurogenesis. May also have a role in tissue modeling during development.UniProtKB/Swiss-Prot Function
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Documents
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"NM_009234" in other vectors (3)
SKU Description Size Price
MG206174 Sox11 (tGFP-tagged) - Mouse SRY-box containing gene 11 (Sox11) 10 ug
$489.00 $657.00
MR206174L3 Lenti ORF clone of Sox11 (Myc-DDK-tagged) - Mouse SRY-box containing gene 11 (Sox11) 10 ug
$757.00
MR206174L4 Lenti ORF clone of Sox11 (mGFP-tagged) - Mouse SRY-box containing gene 11 (Sox11) 10 ug
$757.00

Plasmid Map for MR206174

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206174
Circular map for MR206174

file-alt Citations

Transfection of Sox11 plasmid alleviates ventilator-induced lung injury via Sox11 and FAK
Fang, Fan, Yao et al
Biochem. Biophys. Res. Commun. (2019) 512 (2), 182-188 DOI: 10.1016/j.bbrc.2019.03.041
AI Summary: OriGene's Sox11 plasmid was used to transfect mice in this study, enabling the researchers to upregulate Sox11 expression and investigate its protective effects against ventilator-induced lung injury.
Snippet: Mice in HMV group were received HMV for 4 h. Mice in Sox11 plasmid and Sox11 plasmid + FAK antagonist groups were first administrated with Sox11 plasmid only (10 μg/kg, Origene, USA) or co-administrated with Sox11 plasmid and the antagonist of FAK (PF562271
Showing 1-1 of 1 papers.
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