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Bcat2 (NM_009737) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206133
Bcat2 (Myc-DDK-tagged) - Mouse branched chain aminotransferase 2, mitochondrial (Bcat2), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Bcat2 or NM_009737"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bcat2
Synonyms Bcat(m); Bcat-2; Eca40
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206133 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCAGCCACACTAGGACAGGTCTGGGCCCGAAAGCTTCTGCCTGTTCCCTGGCTTCTATGTGGGT
CCAAAAGATGTGTGTCCTCCATATTCAAGGCTGCAGACCTCCAGATTCAGATGACCAAAGAACCACAGAA
GAAGCCAGCCCCTAGCCAGGCACTGCTGTTTGGGAAGACCTTCACAGACCACATGCTGATGGTGGAGTGG
AATAACAAGGCTGGATGGGGCCCACCAAGGATCCAGCCCTTCCAGAACCTCACGCTACACCCAGCCTGCT
CTGGTCTGCACTACTCTCTGCAGCTCTTTGAGGGCTTGAAAGCATACAAAGGTGGAGACCAGCAGGTACG
CCTCTTCCGGCCCTGGCTCAACATGGACAGGATGCTACGTTCTGCTAGGCGCCTCTGCCTGCCAGACTTT
GACAAGCAGGAGCTGTTGGAGTGTATCCGCCAGCTCATTGAAGTAGACAAAGACTGGGTTCCCGATGGCA
ACGGAACGAGCCTCTACGTGCGGCCCGTGCTGATCGGGAATGAGCCCTCGCTGGGTGTTGGTATGGTCAC
ACAAGCCCTCCTGTACGTCATTCTGTGCCCTGTGGGCTCCTACTTCCCTGGAGACTCCATGACACCTGTC
TCCCTCCTGGCGGACCCTTCATTCGTCAGAGCCTGGATAGGCGGAGTCGGTGACTGCAAGTTGGGGGGGA
ACTATGGACCCACTGTGGCTGTGCAGCGAGAAGCCCAGAAGAGAGGCTGCGAGCAGGTCCTCTGGCTGTA
CGGGCCAGACCACCAGCTCACTGAGGTGGGCACCATGAACATCTTTGTCTACTGGACCCATGAAGACGGA
GTACTGGAGCTGGTAACCCCCCCACTGAATGGCGTCATCTTGCCTGGAGTAGTTCGACAAAGTCTGCTGG
ATCTGGCCAGGACTTGGGGTGAGTTCCGGGTGGCAGAACGCAAGGTCACTATGAAGGAATTGAAGCGGGC
GCTGGAGGAAGGCCGAGTGCGAGAAGTCTTCGGCTCAGGCACTGCTTGTCAGGTCTGCCCGGTGCACCAA
ATCCTGTATGAAGGCAAGCAACTCCACATACCTACCATGGAGAACGGCCCAGAGCTCATCCTGCGCTTCC
AGAAGGAACTGAAGGCTATTCAGTACGGAGCCAGTGCCCACGATTGGATGTTTCGGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206133 protein sequence
Red=Cloning site Green=Tags(s)

MAAATLGQVWARKLLPVPWLLCGSKRCVSSIFKAADLQIQMTKEPQKKPAPSQALLFGKTFTDHMLMVEW
NNKAGWGPPRIQPFQNLTLHPACSGLHYSLQLFEGLKAYKGGDQQVRLFRPWLNMDRMLRSARRLCLPDF
DKQELLECIRQLIEVDKDWVPDGNGTSLYVRPVLIGNEPSLGVGMVTQALLYVILCPVGSYFPGDSMTPV
SLLADPSFVRAWIGGVGDCKLGGNYGPTVAVQREAQKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDG
VLELVTPPLNGVILPGVVRQSLLDLARTWGEFRVAERKVTMKELKRALEEGRVREVFGSGTACQVCPVHQ
ILYEGKQLHIPTMENGPELILRFQKELKAIQYGASAHDWMFRV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009737
ORF Size 1179 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009737.3
RefSeq Size 1780 bp
RefSeq ORF 1182 bp
Locus ID 12036
UniProt ID O35855
Cytogenetics 7 29.36 cM
MW 44.1 kDa
Summary Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009737" in other vectors (4)
SKU Description Size Price
MC201152 Bcat2 (untagged) - Mouse branched chain aminotransferase 2, mitochondrial (Bcat2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$457.00
MG206133 Bcat2 (tGFP-tagged) - Mouse branched chain aminotransferase 2, mitochondrial (Bcat2) 10 ug
$657.00
MR206133L3 Lenti ORF clone of Bcat2 (Myc-DDK-tagged) - Mouse branched chain aminotransferase 2, mitochondrial (Bcat2), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR206133L4 Lenti ORF clone of Bcat2 (mGFP-tagged) - Mouse branched chain aminotransferase 2, mitochondrial (Bcat2), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR206133

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206133
Circular map for MR206133

file-alt Citations

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