Ilkap (NM_023343) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206110
Ilkap (Myc-DDK-tagged) - Mouse integrin-linked kinase-associated serine/threonine phosphatase 2C (Ilkap)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ilkap
Synonyms 0710007A14Rik; 1600009O09Rik; PP2C-DELTA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206110 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCTTTTCGGGGACCTGCCGGAGCCCGAGCGCGCGCCGCGGCCATCTGCCGGGAAGGAAGCACAGG
GACGACCCGTGCTCTTTGAGGACCTGCCCCCGGCCAGCAGTACTGACTCAGGCTCTGGGGGACCTTTACT
CTTTGATGATCTTCCGCCTGCTGCCAGTGGCAATTCAGGTTCTCTTGCCACATCAGGTTCCCAGGTGGTG
AAGACTGAAGGGAAAGGAGCAAAGAGGAAAGCCCCTGAGGAGGAGAAGAATGGCGGTGAAGAGCTTGTGG
AAAAGAAAGTTTGTAAAGCCTCTTCGGTGATCTTTGGTCTGAAAGGCTATGTGGCAGAGCGGAAGGGTGA
GAGGGAGGAGATGCAGGATGCCCATGTCATCCTGAACGATATCACTCAGGAGTGTAATCCTCCATCATCT
CTCATTACTCGGGTTTCATACTTTGCTGTGTTTGATGGACATGGAGGAATACGAGCCTCGAAATTTGCTG
CACAGAATTTGCACCAGAACTTAATCAGGAAATTTCCTAAAGGAGATATAATCAGTGTGGAGAAGACTGT
AAAGAGGTGTCTGCTAGATACTTTTAAGCACACCGATGAAGAGTTCCTGAAACAGGCTTCAAGCCAGAAG
CCTGCCTGGAAAGACGGGTCCACTGCCACGTGTGTCCTGGCTGTGGACAACATCCTGTATATCGCCAACC
TTGGAGATAGTCGGGCAATCCTGTGTCGATATAATGAGGAAAGTCAGAAGCACGCAGCCTTAAGCCTCAG
CAAAGAGCACAACCCAACTCAGTATGAAGAGCGCATGAGGATACAGAAAGCTGGAGGGAATGTCAGAGAT
GGGCGTGTCTTGGGCGTGCTGGAGGTATCGCGTTCCATTGGAGATGGGCAGTACAAGCGCTGTGGGGTCA
CATCTGTGCCTGATATCAGACGCTGCCAGCTGACCCCCAATGACAGGTTCATTTTGCTGGCTTGCGACGG
GCTTTTCAAGGTCTTTACCCCAGAAGAAGCTGTGAACTTCATCTTGTCCTGCCTTGAGGATGACAAGATC
CAGACCCGGGAAGGGAAGCCTGCTGTTGATGCCCGCTATGAAGCTGCATGCAACAGGCTGGCCAACAAGG
CAGTGCAGCGGGGCTCAGCAGACAACGTGACGGTGATGGTGGTGAGGATAGGACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206110 protein sequence
Red=Cloning site Green=Tags(s)

MDLFGDLPEPERAPRPSAGKEAQGRPVLFEDLPPASSTDSGSGGPLLFDDLPPAASGNSGSLATSGSQVV
KTEGKGAKRKAPEEEKNGGEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITQECNPPSS
LITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDIISVEKTVKRCLLDTFKHTDEEFLKQASSQK
PAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRD
GRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDDKI
QTREGKPAVDARYEAACNRLANKAVQRGSADNVTVMVVRIGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023343
ORF Size 1176 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023343.1
RefSeq Size 1368 bp
RefSeq ORF 1179 bp
Locus ID 67444
UniProt ID Q8R0F6
Cytogenetics 1 D
MW 42.8 kDa
Summary Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023343" in other vectors (4)
SKU Description Size Price
MC204065 Ilkap (untagged) - Mouse integrin-linked kinase-associated serine/threonine phosphatase 2C (Ilkap), (10ug) 10 ug
$457.00
MG206110 Ilkap (tGFP-tagged) - Mouse integrin-linked kinase-associated serine/threonine phosphatase 2C (Ilkap) 10 ug
$657.00
MR206110L3 Lenti ORF clone of Ilkap (Myc-DDK-tagged) - Mouse integrin-linked kinase-associated serine/threonine phosphatase 2C (Ilkap) 10 ug
$757.00
MR206110L4 Lenti ORF clone of Ilkap (mGFP-tagged) - Mouse integrin-linked kinase-associated serine/threonine phosphatase 2C (Ilkap) 10 ug
$757.00

Plasmid Map for MR206110

Download sequence for

Cloning scheme for MR206110

Circular map for MR206110

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