Dpf2 (NM_011262) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206101
Dpf2 (Myc-DDK-tagged) - Mouse D4, zinc and double PHD fingers family 2 (Dpf2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dpf2
Synonyms 2210010M07Rik; BAF45D; Req; ubi-d4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206101 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGTGGTGGAGAATGTAGTGAAGCTCCTTGGCGAGCAATACTACAAAGATGCCATGGAACAGT
GCCACAATTATAACGCCCGCCTCTGTGCTGAACGTAGTGTGCGCCTGCCTTTCCTGGACTCACAGACTGG
AGTAGCCCAGAGCAATTGTTATATCTGGATGGAAAAGCGACACCGGGGACCAGGATTGGCCTCTGGACAG
TTATACTCCTATCCTGCCAGACGCTGGCGGAAAAAGCGCCGAGCCCACCCACCTGAGGATCCCAGGCTTT
CTTTCCCATCGATTAAACCAGACACTGACCAGACTCTGAAGAAAGAGGGGCTTATCTCTCAGGATGGCAG
CAGTTTAGAGGCTCTGTTGCGTACTGATCCCCTGGAGAAACGGGGTGCCCCAGATCCCCGAGTTGACGAT
GACAGCCTGGGCGAGTTTCCTGTTAGCAACAGTCGAGCACGGAAGCGGATCATTGAACCCGATGACTTCC
TTGATGACCTTGATGATGAGGACTATGAAGAAGATACGCCAAAGCGTCGGGGGAAGGGGAAGTCCAAGAG
TAAGGGTGTGAGCAGTGCCCGGAAGAAGCTGGATGCTTCCATCCTGGAGGACCGGGATAAGCCCTATGCC
TGTGACATTTGTGGAAAACGTTACAAGAACCGACCTGGCCTCAGTTACCACTATGCCCACTCCCACCTGG
CTGAAGAGGAAGGAGAGGACAAAGAAGACTCCCGACCCCCCACTCCTGTGTCCCAGAGGTCTGAGGAGCA
GAAATCCAAGAAAGGACCTGATGGATTGGCCCTGCCTAACAACTACTGTGACTTCTGCCTAGGAGACTCA
AAAATCAACAAGAAGACAGGGCAGCCCGAGGAGCTAGTGTCCTGTTCCGACTGTGGCCGCTCAGGGCATC
CGTCCTGCCTGCAGTTCACCCCTGTGATGATGGCGGCCGTGAAGACCTACCGCTGGCAGTGCATCGAATG
CAAGTGCTGCAACCTCTGCGGCACGTCGGAGAACGACGACCAGCTACTTTTCTGTGATGACTGTGACCGT
GGCTACCACATGTACTGTCTCACTCCTTCCATGTCTGAGCCTCCTGAAGGAAGTTGGAGTTGCCACCTGT
GTCTGGATCTGCTGAAGGAGAAAGCATCCATCTACCAGAACCAGAACTCCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206101 protein sequence
Red=Cloning site Green=Tags(s)

MAAVVENVVKLLGEQYYKDAMEQCHNYNARLCAERSVRLPFLDSQTGVAQSNCYIWMEKRHRGPGLASGQ
LYSYPARRWRKKRRAHPPEDPRLSFPSIKPDTDQTLKKEGLISQDGSSLEALLRTDPLEKRGAPDPRVDD
DSLGEFPVSNSRARKRIIEPDDFLDDLDDEDYEEDTPKRRGKGKSKSKGVSSARKKLDASILEDRDKPYA
CDICGKRYKNRPGLSYHYAHSHLAEEEGEDKEDSRPPTPVSQRSEEQKSKKGPDGLALPNNYCDFCLGDS
KINKKTGQPEELVSCSDCGRSGHPSCLQFTPVMMAAVKTYRWQCIECKCCNLCGTSENDDQLLFCDDCDR
GYHMYCLTPSMSEPPEGSWSCHLCLDLLKEKASIYQNQNSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011262
ORF Size 1173 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011262.1
RefSeq Size 3314 bp
RefSeq ORF 1176 bp
Locus ID 19708
UniProt ID Q61103
Cytogenetics 19 4.34 cM
MW 44.2 kDa
Summary Plays an active role in transcriptional regulation by binding modified histones H3 and H4. Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (By similarity). Might also have a role in the development and maturation of lymphoid cells (PubMed:7961935). Involved in the regulation of non-canonical NF-kappa-B pathway (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011262" in other vectors (4)
SKU Description Size Price
MC200792 Dpf2 (untagged) - Mouse D4, zinc and double PHD fingers family 2 (Dpf2), (10ug) 10 ug
$457.00
MG206101 Dpf2 (tGFP-tagged) - Mouse D4, zinc and double PHD fingers family 2 (Dpf2) 10 ug
$657.00
MR206101L3 Lenti ORF clone of Dpf2 (Myc-DDK-tagged) - Mouse D4, zinc and double PHD fingers family 2 (Dpf2) 10 ug
$757.00
MR206101L4 Lenti ORF clone of Dpf2 (mGFP-tagged) - Mouse D4, zinc and double PHD fingers family 2 (Dpf2) 10 ug
$757.00

Plasmid Map for MR206101

Download sequence for

Cloning scheme for MR206101

Circular map for MR206101

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