Rrm2 (NM_009104) Mouse Tagged ORF Clone

SKU
MR206082
Rrm2 (Myc-DDK-tagged) - Mouse ribonucleotide reductase M2 (Rrm2)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$289.00 MSRP $457.00 MSRP $457.00
In Stock*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rrm2
Synonyms AA407299; R2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206082 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCTCCGTCCGCACCCCGCTCGCCACCATCGCTGACCAGCAGCAGCTGCAGTTGTCGCCGCTGAAGC
GACTCACCCTGGCTGACAAGGAGAACACGCCCCCGACTCTCAGCAGCACCCGCGTCCTGGCCAGCAAAGC
TGCGAGGAGAATCTTCCAGGACTCCGCCGAGCTGGAAAGTAAAGCGCCTACTAACCCCAGCGTTGAGGAT
GAGCCGTTACTGAGAGAAAACCCCCGCCGCTTCGTTGTCTTTCCCATCGAGTACCATGATATCTGGCAGA
TGTACAAGAAAGCCGAGGCCTCCTTTTGGACTGCCGAGGAGGTGGACCTTTCCAAGGATATTCAGCACTG
GGAAGCTCTGAAACCCGATGAGAGACATTTTATATCTCACGTTCTGGCTTTCTTTGCAGCGAGTGATGGC
ATAGTCAATGAGAACTTGGTGGAGCGATTTAGCCAAGAAGTTCAAGTTACAGAGGCCCGCTGTTTCTATG
GCTTCCAAATTGCCATGGAAAACATACACTCTGAAATGTACAGTCTCCTTATTGACACTTACATTAAAGA
TCCCAAGGAAAGAGAATATCTCTTCAATGCTATTGAAACAATGCCTTGTGTGAAGAAGAAGGCTGACTGG
GCCTTGCGCTGGATTGGGGACAAAGAGGCTACGTATGGAGAACGCGTTGTGGCCTTTGCCGCCGTAGAAG
GAATCTTCTTTTCCGGTTCTTTTGCATCGATATTCTGGCTCAAGAAACGGGGGCTGATGCCGGGCCTTAC
ATTTTCCAATGAGCTTATTAGCAGAGACGAGGGTTTACACTGTGACTTTGCCTGCCTGATGTTCAAGCAC
CTGGTACACAAGCCAGCAGAGCAGAGGGTCCGAGAGATAATCACCAACGCCGTTAGGATAGAGCAGGAGT
TCCTCACGGAGGCCTTGCCCGTGAAGCTCATCGGGATGAACTGCACTTTGATGAAGCAGTACATTGAGTT
TGTGGCCGACAGGCTTATGCTGGAGCTGGGTTTTAACAAGATTTTCAGAGTAGAAAATCCGTTTGACTTC
ATGGAAAATATCTCACTAGAAGGAAAGACAAACTTCTTTGAGAAGCGAGTAGGCGAGTATCAGAGGATGG
GAGTCATGTCGAATTCGACAGAGAACTCTTTTACCTTGGATGCTGACTTC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206082 protein sequence
Red=Cloning site Green=Tags(s)

MLSVRTPLATIADQQQLQLSPLKRLTLADKENTPPTLSSTRVLASKAARRIFQDSAELESKAPTNPSVED
EPLLRENPRRFVVFPIEYHDIWQMYKKAEASFWTAEEVDLSKDIQHWEALKPDERHFISHVLAFFAASDG
IVNENLVERFSQEVQVTEARCFYGFQIAMENIHSEMYSLLIDTYIKDPKEREYLFNAIETMPCVKKKADW
ALRWIGDKEATYGERVVAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFKH
LVHKPAEQRVREIITNAVRIEQEFLTEALPVKLIGMNCTLMKQYIEFVADRLMLELGFNKIFRVENPFDF
MENISLEGKTNFFEKRVGEYQRMGVMSNSTENSFTLDADF

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene Plasmid Map
ACCN NM_009104
ORF Size 1170 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009104.2
RefSeq Size 2199 bp
RefSeq ORF 1173 bp
Locus ID 20135
UniProt ID P11157
Cytogenetics 12 8.5 cM
MW 45.1 kDa
Summary Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling (By similarity).[UniProtKB/Swiss-Prot Function]
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MR206082L2 Lenti ORF clone of Rrm2 (mGFP-tagged) - Mouse ribonucleotide reductase M2 (Rrm2) 10 ug
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MR206082L3 Lenti ORF clone of Rrm2 (Myc-DDK-tagged) - Mouse ribonucleotide reductase M2 (Rrm2) 10 ug
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MR206082L4 Lenti ORF clone of Rrm2 (mGFP-tagged) - Mouse ribonucleotide reductase M2 (Rrm2) 10 ug
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Citations

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