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Adap2 (NM_172133) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205955
Adap2 (Myc-DDK-tagged) - Mouse ArfGAP with dual PH domains 2 (Adap2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adap2
Synonyms Centa2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205955 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGACCGCGAGCGCAACAAGAAGCGGCTGCTGGAGCTGCTGCAGGCTGCGGGCACCGGCAACGGGC
ACTGCGCCGACTGCGGGGCAGCGGATCCTGATTGGGCCTCTTACAAGCTGGGGATTTTCATCTGTCTCCA
CTGCTCTGGCGTCCACCGCAACTTCCCAGACATCAGCAAAGTTAAATCCGTGAGACTGGACTTCTGGGAC
GACAGTATGGTGGAGTTCATGACTCACCATGGGAACCTCAACGTGAAAGCCAAGTTTGAAGCAAGAGTCC
CAGCTTTCTACTATGTCCCTCAGGCCAACGACTGCCTGGTCTTAAAGGAACAATGGATTCGAGCTAAGTA
TGAAAGACAGGAGTTTACGGCTATTGACAAAGCTGTCTCACATCCAGGTAACCGAGAAGGGTTCCTGTGG
AAGCGAGGACGGGACAACGCACAGTTCCTGAGAAGGCGGTTTGTCCTTCTGTCGAGGGAAGGCCTCCTGA
AGTACTACACCAAGGAGGAGGGTAAAGCCCCAAAGGCTGTCATCAGCATCAAGGACTTAAACGCCACCTT
CCAGACAGAGAAGATAGGACATCCCCACGGGCTGCAGATCACCTACAGGAAAGAGGGCCACACCAGGAAC
CTGTTTGTTTATCATGACAGTGGGAAGGAGATAGTGGATTGGTTCAATGCTCTCCGTGCAGCCCGCCTGC
AATACCTGAAATTGGCCTTTCCTGATCTCCCAGAGTCTGAGCTCGTGCCTCTCATCACCAGGAACTACCT
CAAACAAGGCTTCATGGAGAAGACTGGTCCAAAGCATAGAGAACCCTTCAAGAAAAGATGGTTTGCCTTG
GATCCGCAGGAACGGAGGCTGCTCTATTATAAGAACCCCCTGGATGCTTTTGAGCTGGGCCAGGTTTTTC
TGGGAAGCAATGAGCAGGGATATGAAGTATGGGAAGATCTGCCCAAGGGCATCCGAGGGAACCGCTGGAA
GGCTGGACTTACCGTCATCACTCCGGAGCGGAAATTCATCTTCACCTGCCCCACTGAAAAGGAACAGCGA
GAATGGCTAGAGAGTCTGCGGGGCGTCTTGTCCAGTCCCTTGTCACCCCTCCACCTCCTCACCACATCAG
CAGAGACTGGCTGTGGCCTGGGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205955 protein sequence
Red=Cloning site Green=Tags(s)

MGDRERNKKRLLELLQAAGTGNGHCADCGAADPDWASYKLGIFICLHCSGVHRNFPDISKVKSVRLDFWD
DSMVEFMTHHGNLNVKAKFEARVPAFYYVPQANDCLVLKEQWIRAKYERQEFTAIDKAVSHPGNREGFLW
KRGRDNAQFLRRRFVLLSREGLLKYYTKEEGKAPKAVISIKDLNATFQTEKIGHPHGLQITYRKEGHTRN
LFVYHDSGKEIVDWFNALRAARLQYLKLAFPDLPESELVPLITRNYLKQGFMEKTGPKHREPFKKRWFAL
DPQERRLLYYKNPLDAFELGQVFLGSNEQGYEVWEDLPKGIRGNRWKAGLTVITPERKFIFTCPTEKEQR
EWLESLRGVLSSPLSPLHLLTTSAETGCGLG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172133
ORF Size 1143 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172133.1, NP_742145.1
RefSeq Size 1611 bp
RefSeq ORF 1146 bp
Locus ID 216991
UniProt ID Q8R2V5
Cytogenetics 11 47.56 cM
MW 44 kDa
Summary GTPase-activating protein for the ADP ribosylation factor family (Potential). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Possesses a stoichiometry of two binding sites for InsP4 with identical affinity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_172133" in other vectors (4)
SKU Description Size Price
MC203184 Adap2 (untagged) - Mouse ArfGAP with dual PH domains 2 (Adap2), (10ug) 10 ug
$457.00
MG205955 Adap2 (tGFP-tagged) - Mouse centaurin, alpha 2 (Centa2) 10 ug
$657.00
MR205955L3 Lenti ORF clone of Adap2 (Myc-DDK-tagged) - Mouse ArfGAP with dual PH domains 2 (Adap2) 10 ug
$757.00
MR205955L4 Lenti ORF clone of Adap2 (mGFP-tagged) - Mouse ArfGAP with dual PH domains 2 (Adap2) 10 ug
$757.00

Plasmid Map for MR205955

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205955
Circular map for MR205955

file-alt Citations

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