Ptx3 (NM_008987) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205951
Ptx3 (Myc-DDK-tagged) - Mouse pentraxin related gene (Ptx3)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptx3
Synonyms AI607804; TSG-14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205951 representing NM_008987
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACCTCCCTGCGATCCTGCTTTGTGCTCTCTGGTCTGCAGTAGTGGCTGAGACCTCGGATGACTACG
AGCTCATGTATGTGAATTTGGACAACGAAATAGACAATGGACTTCATCCCACCGAGGACCCCACGCCATG
CGACTGCCGCCAGGAGCACTCGGAGTGGGACAAGCTGTTCATCATGCTGGAGAACTCGCAGATGCGGGAG
GGCATGCTGTTGCAGGCCACCGACGACGTCCTCCGTGGAGAGCTGCAGAGGCTGCGGGCAGAGCTGGGGC
GGCTGGCGGGCGGCATGGCGAGGCCGTGCGCAGCCGGTGGCCCCGCAGACGCCAGGCTGGTGCGGGCGCT
GGAGCCGCTGCTGCAGGAGAGCCGTGACGCGAGCCGCAGGCTGGCGCGCCTGGAGGACGCGGAGGCGCGG
CGATCCGAGGCGACAGTGTCTGGCCTAGGCGCTGTGCTGGAGGAACTGCGGCGGACGCGCGCCGACCTGA
GCGCAGTGCAGAGCTGGGTCGCCCGCCACTGGCTGCCCGCAGGTTGTGAAACAGCAATTCTCTTCCCAAT
GCGTTCGAAGAAGATTTTTGGAAGCGTGCATCCTGTGAGACCAATGAAGCTTGAATCTTTTAGTACTTGC
ATTTGGGTCAAAGCCACAGATGTATTAAACAAAACCATCCTGTTTTCTTATGGCACAAAGTGGAACCCCT
ATGAGATTCAGCTGTACCTCAGTTCCCAGTCCCTAGTGTTGGTGGTGGGTGGAAAGGAGAACAAGCTGGC
TGCAGACACTGTGGTGTCCCTGGGGAGGTGGTCCCACCTGTGTGGCACCTGGAGTTCAGAGCAGGGGAGC
ATGTCCCTGTGGGCAAACGGGGAGCTGGTGGCTACCACTGTAGAGATGGCCAAAAGTCACTCTGTTCCTG
AGGGTGGACTCCTACAGATTGGCCAAGAAAAGAATGGTTGCTGTGTAGGTGGGGGCTTTGACGAATCATT
AGCATTTTCTGGAAGAATCACAGGCTTCAATATCTGGGATCGGGTTCTCAGCGAGGAGGAGATACGGGCC
AGTGGAGGAGTCGAATCCTGTCACATCCGGGGAAATGTCGTCGGGTGGGGAGTCACAGAGATTCAGGCGC
ACGGAGGAGCCCAGTATGTTTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205951 representing NM_008987
Red=Cloning site Green=Tags(s)

MHLPAILLCALWSAVVAETSDDYELMYVNLDNEIDNGLHPTEDPTPCDCRQEHSEWDKLFIMLENSQMRE
GMLLQATDDVLRGELQRLRAELGRLAGGMARPCAAGGPADARLVRALEPLLQESRDASRRLARLEDAEAR
RSEATVSGLGAVLEELRRTRADLSAVQSWVARHWLPAGCETAILFPMRSKKIFGSVHPVRPMKLESFSTC
IWVKATDVLNKTILFSYGTKWNPYEIQLYLSSQSLVLVVGGKENKLAADTVVSLGRWSHLCGTWSSEQGS
MSLWANGELVATTVEMAKSHSVPEGGLLQIGQEKNGCCVGGGFDESLAFSGRITGFNIWDRVLSEEEIRA
SGGVESCHIRGNVVGWGVTEIQAHGGAQYVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008987
ORF Size 1143 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008987.2
RefSeq Size 1924 bp
RefSeq ORF 1146 bp
Locus ID 19288
UniProt ID P48759
Cytogenetics 3 30.25 cM
MW 42.3 kDa
Summary Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_008987" in other vectors (4)
SKU Description Size Price
MC200901 Ptx3 (untagged) - Mouse pentraxin related gene (Ptx3), (10ug) 10 ug
$686.00
MG205951 Ptx3 (tGFP-tagged) - Mouse pentraxin related gene (Ptx3) 10 ug
$886.00
MR205951L3 Lenti ORF clone of Ptx3 (Myc-DDK-tagged) - Mouse pentraxin related gene (Ptx3) 10 ug
$986.00
MR205951L4 Lenti ORF clone of Ptx3 (mGFP-tagged) - Mouse pentraxin related gene (Ptx3) 10 ug
$986.00

Plasmid Map for MR205951

Download sequence for

Cloning scheme for MR205951

Circular map for MR205951

file-alt Citations

Comprehensive Molecular Profiles of Functionally Effective MSC-Derived Extracellular Vesicles in Immunomodulation
Kim, Lee, Bae et al
Mol Ther (2020) 28 (7), 1628-1644 DOI: 10.1016/j.ymthe.2020.04.020
Cited by 146
AI Summary: OriGene products, specifically the human PTX3 cDNA clone (RC209875) and TGF-β1 shRNA plasmids (TL312463V), were used in this study to manipulate expression levels of key molecules in MSCs, enabling the authors to assess the roles of PTX3 and TGF-β1 in the immunomodulatory effects of MSC-derived extracellular vesicles.
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products