Serpinb7 (NM_027548) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205936
Serpinb7 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 7 (Serpinb7)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Serpinb7
Synonyms 4631416M05Rik; megsin; ovalbumin
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205936 representing NM_027548
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCCCTTGCTGCAGCAAATGCAGAATTTGGCTTCGACTTATTCAGAGAGATGGATAGTAGCCAAG
GAAATGGAAATGTATTCTTCTCTTCCCTGAGCATCTTCACTGCCCTGACCCTAATCCGTCTGGGTGCTCG
AGGTGACTGTGCACGTCAGATTGACAAGGCACTGCACTTTAACATACCATCAAGACAAGGAAACTCATCT
AATAATCAGCCAGGACTTCAGTATCAATTGAAAAGAGTTCTTGCTGACATAAACTCATCTCATAAGGATT
ATGAACTCAGCATTGCCACTGGAGTTTTTGCAGAAAAAGTCTATGACTTTCATAAGAACTACATTGAGTG
TGCTGAAAACTTATACAATGCTAAAGTGGAAAGAGTTGACTTCACAAATGATGTACAAGATACCAGATTT
AAAATTAATAAATGGATTGAAAATGAGACACATGGAAAGATCAAGAAGGTGTTGGGCGACAGCAGCCTCA
GCTCGTCGGCTGTCATGGTGCTGGTGAACGCTGTTTACTTCAAAGGCAAATGGAAATCGGCCTTCACCAA
GACTGATACCCTCAGTTGCCGTTTTAGGTCTCCCACGTGTCCTGGAAAAGTAGTTAATATGATGCATCAA
GAACGGCGGTTCAATTTGTCTACCATTCAGCAGCCACCAATGCAGGTTCTTGAGCTCCAATATCATGGTG
GCATAAGCATGTACATTATGCTGCCTGAGGATGGCCTATGTGAAATTGAAAGCAAGCTGAGTTTCCAGAA
TCTGATGGACTGGACCAATAGGAGGAAAATGAAATCTCAGTATGTGAACGTGTTTCTCCCCCAGTTCAAG
ATAGAGAAGAATTATGAAATGACGCACCACTTGAAATCCTTAGGCTTGAAAGATATCTTTGATGAGTCCA
GTGCAGATCTCTCTGGAATTGCCTCTGGAGGTCGTCTCTACGTATCAAAGCTAATGCACAAGTCATTCAT
AGAGGTCTCAGAGGAGGGCACTGAAGCCACTGCTGCCACAGAAAATAACATTGTTGAAAAGCAGCTTCCT
GAGTCCACAGTGTTCAGAGCCGACCGCCCCTTTCTGTTTGTCATCAAGAAGAATGACATCATCTTATTTA
CTGGCAAAGTCTCTTGTCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205936 representing NM_027548
Red=Cloning site Green=Tags(s)

MASLAAANAEFGFDLFREMDSSQGNGNVFFSSLSIFTALTLIRLGARGDCARQIDKALHFNIPSRQGNSS
NNQPGLQYQLKRVLADINSSHKDYELSIATGVFAEKVYDFHKNYIECAENLYNAKVERVDFTNDVQDTRF
KINKWIENETHGKIKKVLGDSSLSSSAVMVLVNAVYFKGKWKSAFTKTDTLSCRFRSPTCPGKVVNMMHQ
ERRFNLSTIQQPPMQVLELQYHGGISMYIMLPEDGLCEIESKLSFQNLMDWTNRRKMKSQYVNVFLPQFK
IEKNYEMTHHLKSLGLKDIFDESSADLSGIASGGRLYVSKLMHKSFIEVSEEGTEATAATENNIVEKQLP
ESTVFRADRPFLFVIKKNDIILFTGKVSCP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027548
ORF Size 1140 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027548.3, NP_081824.1
RefSeq Size 1894 bp
RefSeq ORF 1143 bp
Locus ID 116872
UniProt ID Q9D695
Cytogenetics 1 E2.1
MW 43.1 kDa
Summary Might function as an inhibitor of Lys-specific proteases. Might influence the maturation of megakaryocytes via its action as a serpin (By similarity).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_027548" in other vectors (3)
SKU Description Size Price
MG205936 Serpinb7 (tGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 7 (Serpinb7) 10 ug
$657.00
MR205936L3 Lenti ORF clone of Serpinb7 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 7 (Serpinb7) 10 ug
$757.00
MR205936L4 Lenti ORF clone of Serpinb7 (mGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 7 (Serpinb7) 10 ug
$757.00

Plasmid Map for MR205936

Download sequence for

Cloning scheme for MR205936

Circular map for MR205936

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products