Acta2 (NM_007392) Mouse Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Acta2 |
| Synonyms | 0610041G09Rik; a-; a-SMA; Ac; Actvs; al; alphaSMA; SM; SMAalpha; SMalphaA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007392 |
| ORF Size | 1131 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007392.1, NM_007392.2, NM_007392.3, NP_031418.1 |
| RefSeq Size | 2572 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 11475 |
| UniProt ID | P62737 |
| Cytogenetics | 19 C1 |
| MW | 42 kDa |
| Summary | The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in smooth muscle. provided by RefSeq, Feb 2021 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007392" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC204777 | Acta2 (untagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2), (10ug) | 10 ug |
$686.00
|
|
| MG205873 | Acta2 (tGFP-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2) | 10 ug |
$886.00
|
|
| MR205873L1 | Lenti ORF clone of Acta2 (Myc-DDK-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2) | 10 ug |
$986.00
|
|
| MR205873L2 | Lenti ORF clone of Acta2 (mGFP-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2) | 10 ug |
$986.00
|
|
| MR205873L3 | Lenti ORF clone of Acta2 (Myc-DDK-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2) | 10 ug |
$986.00
|
|
| MR205873L4 | Lenti ORF clone of Acta2 (mGFP-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2) | 10 ug |
$986.00
|
|
Citations
Collateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profile
Lemaitre, Hurtado, De Gregorio et al
Mol. Neurobiol. (2020) 57 (10), 4232-4249 DOI: 10.1007/s12035-020-01986-3
Lemaitre, Hurtado, De Gregorio et al
Mol. Neurobiol. (2020) 57 (10), 4232-4249 DOI: 10.1007/s12035-020-01986-3
Cited by 29
AI Summary: OriGene's GFP-tagged plasmids for Atf3 (cat no. MR201634), Fgf3 (cat no. MR222923), and Gdf10 were used in this study to genetically upregulate candidate genes and assess their roles in promoting neuronal branching during collateral sprouting of sensory neurons.
Snippet: 30530) and 5 μg of the following genes plasmids tagged with green fluorescent protein (GFP): Atf3 (Origene, cat no. MR201634), fibroblast growth factor 3 (Fgf3) (Origene, cat no. MR222923), growth differentiation factor 10 (Gdf10) (Origene, cat no;- 30530) and 5 μg of the following genes plasmids tagged with green fluorescent protein (GFP): Atf3 (Origene, cat no. MR201634), fibroblast growth factor 3 (Fgf3) (Origene, cat no. MR222923), growth differentiation factor 10 (Gdf10) (Origene, cat no
The role of α-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling
Shinde, Humeres, Frangogiannis
Biochim. Biophys. Acta (2016) 1863 (1), 298-309 DOI: 10.1016/j.bbadis.2016.11.006
Shinde, Humeres, Frangogiannis
Biochim. Biophys. Acta (2016) 1863 (1), 298-309 DOI: 10.1016/j.bbadis.2016.11.006
Cited by 589
AI Summary: In this study, OriGene Technologies' SMA cDNA was used to overexpress α-smooth muscle actin in mouse cardiac fibroblasts, enabling the authors to investigate the functional role of α-SMA in fibroblast-mediated matrix contraction and remodeling.
Snippet: For -SMA overexpression experiments, mouse cardiac fibroblasts at passage1 were seeded at 80% confluence (10 cm dishes) in complete medium and were either transfectedwith 2.5 ng of SMA cDNA (Origene Technologies) or transfected with a control entry ... Cite SaveSaving
Showing 1-2 of 2 papers.
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