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Tdh (NM_021480) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205787
Tdh (Myc-DDK-tagged) - Mouse L-threonine dehydrogenase (Tdh)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tdh
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205787 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTATTCCTTGGGATGCTAAAACAGGTGGTCAATGGGACTGCACAGAGCAAAGCCTCTAGCTGCCGGA
AGCTTGTCTTGCCCTTGAAGTTTCTAGGCACCTCCCAGCACCGGATCCCAGCCGATGCCAACTTCCACTC
CACCTCGATCTCGGAAGCTGAGCCTCCTCGGGTCTTGATCACAGGGGGTCTTGGCCAGCTGGGGGTAGGA
CTTGCTAACCTCCTGAGGAAACGATTTGGGAAGAACAATGTGATCCTATCAGACATAAGGAAGCCCCCTG
CTCATGTCTTCCACAGTGGCCCGTTTGTTTATGCCAACATCTTGGATTACAAGAGCCTCCGTGAGATCGT
GGTGAACCACCGTATCAGCTGGCTGTTTCACTACAGTGCCCTGCTGAGTGCCGTGGGAGAGGCAAATGTG
TCCTTAGCCAGAGATGTGAATATAACTGGATTGCATAACGTTCTAGATGTTGCAGCTGAATACAACGTGA
GATTGTTTGTGCCCAGCACGATTGGAGCCTTTGGACCTACCTCTCCCCGGAACCCTGCACCCGATCTCTG
TATTCAAAGGCCTAGGACCATCTATGGGGTATCCAAGGTCCACACAGAGCTCATGGGAGAATACTATTAT
TACCGGTACGGGTTAGATTTTCGGTGCCTGCGTTATCCTGGAATCATTTCTGCAGATTCCCAGCCTGGAG
GAGGAACAACTGACTACGCCGTCCAGATTTTCCACGCTGCAGCAAAGAACGGCACATTCGAGTGCAACCT
CGAGGCTGGCACCAGGCTCCCAATGATGTACATCAGTGACTGCCTCAGGGCCACCCTGGAGGTCATGGAA
GCCCCTGCCGAGCGCCTGTCCATGAGAACCTACAACATCAGCGCCATGAGCTTCACCCCCGAAGAGCTGG
CCCAGGCGCTCCGCAAGCACGCGCCTGACTTCCAGATCACCCACTGCGTGGATCCCCTCCGACAGGCCAT
AGCGGAGAGCTGGCCCATGATTCTAGATGACAGCAATGCTCGGAAGGACTGGGGCTGGAAGCATGACTTT
GATCTTCCAGAGTTGGTGGCTACGATGCTCAACTTCCACGGCGTCAGCACCAGAGTTGCCCAAGTGAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205787 protein sequence
Red=Cloning site Green=Tags(s)

MLFLGMLKQVVNGTAQSKASSCRKLVLPLKFLGTSQHRIPADANFHSTSISEAEPPRVLITGGLGQLGVG
LANLLRKRFGKNNVILSDIRKPPAHVFHSGPFVYANILDYKSLREIVVNHRISWLFHYSALLSAVGEANV
SLARDVNITGLHNVLDVAAEYNVRLFVPSTIGAFGPTSPRNPAPDLCIQRPRTIYGVSKVHTELMGEYYY
YRYGLDFRCLRYPGIISADSQPGGGTTDYAVQIFHAAAKNGTFECNLEAGTRLPMMYISDCLRATLEVME
APAERLSMRTYNISAMSFTPEELAQALRKHAPDFQITHCVDPLRQAIAESWPMILDDSNARKDWGWKHDF
DLPELVATMLNFHGVSTRVAQVN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021480
ORF Size 1119 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021480.4, NP_067455.4
RefSeq Size 1900 bp
RefSeq ORF 1122 bp
Locus ID 58865
UniProt ID Q8K3F7
Cytogenetics 14 D1
MW 41.4 kDa
Summary Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate, mediating L-threonine catabolism.UniProtKB/Swiss-Prot Function
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Documents
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"NM_021480" in other vectors (4)
SKU Description Size Price
MC202018 Tdh (untagged) - Mouse L-threonine dehydrogenase (Tdh), (10ug) 10 ug
$457.00
MG205787 Tdh (tGFP-tagged) - Mouse L-threonine dehydrogenase (Tdh) 10 ug
$657.00
MR205787L3 Lenti ORF clone of Tdh (Myc-DDK-tagged) - Mouse L-threonine dehydrogenase (Tdh) 10 ug
$757.00
MR205787L4 Lenti ORF clone of Tdh (mGFP-tagged) - Mouse L-threonine dehydrogenase (Tdh) 10 ug
$757.00

Plasmid Map for MR205787

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205787
Circular map for MR205787

file-alt Citations

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