P2ry2 (NM_008773) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205778
P2ry2 (Myc-DDK-tagged) - Mouse purinergic receptor P2Y, G-protein coupled 2 (P2ry2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol P2ry2
Synonyms P2U1; P2Y2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205778 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCAGACCTGGAACCCTGGAATAGCACCATCAATGGCACCTGGGAGGGGGACGAACTGGGATACA
AGTGTCGCTTCAACGAGGACTTCAAGTACGTGCTGTTGCCCGTGTCCTATGGCGTGGTGTGCGTGCTCGG
GTTGTGCCTGAACGTCGTGGCTCTCTATATCTTCCTATGCCGCCTCAAAACCTGGAACGCCTCCACCACC
TACATGTTTCACCTGGCAGTTTCGGACTCTCTCTACGCAGCGTCCCTGCCGCTGTTGGTTTATTACTACG
CCCGGGGTGACCACTGGCCATTTAGCACGGTGCTCTGCAAGCTGGTGCGTTTCCTCTTCTACACCAACCT
CTACTGCAGCATCCTCTTCCTCACCTGCATCAGCGTGCACCGGTGCCTGGGAGTCCTGCGCCCTCTGCAC
TCCCTGCGTTGGGGCCGGGCCCGTTATGCCCGCCGGGTGGCTGCGGTTGTGTGGGTGCTGGTGCTGGCCT
GCCAGGCACCCGTGCTCTACTTCGTCACCACCAGCGTGCGGGGAACCCGGATCACTTGCCATGACACCTC
GGCCCGAGAGCTCTTTAGCCATTTTGTGGCTTACAGCTCCGTCATGCTGGGTCTGCTTTTTGCTGTGCCC
TTTTCCGTAATCCTGGTCTGTTACGTGCTTATGGCCAGGCGGCTGCTCAAACCGGCTTATGGGACCACAG
GAGGTCTGCCTCGGGCCAAGCGCAAGTCTGTGCGCACCATTGCCTTGGTACTGGCCGTCTTCGCCCTCTG
CTTTCTGCCTTTCCACGTCACGCGCACCCTCTACTACTCCTTCCGATCACTTGACCTCAGCTGCCACACC
CTCAACGCCATCAACATGGCATATAAGATCACCCGGCCGCTGGCCAGCGCCAACAGTTGTCTTGACCCGG
TACTCTACTTCCTGGCAGGGCAGAGACTTGTCCGCTTTGCCCGAGATGCCAAGCCACCCACGGAGCCTAC
CCCCAGCCCACAGGCTCGTCGCAAGCTGGGCCTGCACAGGCCTAACAGAACTGTGAGGAAAGATTTGTCA
GTCAGCAGTGACGACTCAAGACGGACAGAGTCCACACCAGCTGGAAGTGAGACTAAGGACATTCGGCTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205778 protein sequence
Red=Cloning site Green=Tags(s)

MAADLEPWNSTINGTWEGDELGYKCRFNEDFKYVLLPVSYGVVCVLGLCLNVVALYIFLCRLKTWNASTT
YMFHLAVSDSLYAASLPLLVYYYARGDHWPFSTVLCKLVRFLFYTNLYCSILFLTCISVHRCLGVLRPLH
SLRWGRARYARRVAAVVWVLVLACQAPVLYFVTTSVRGTRITCHDTSARELFSHFVAYSSVMLGLLFAVP
FSVILVCYVLMARRLLKPAYGTTGGLPRAKRKSVRTIALVLAVFALCFLPFHVTRTLYYSFRSLDLSCHT
LNAINMAYKITRPLASANSCLDPVLYFLAGQRLVRFARDAKPPTEPTPSPQARRKLGLHRPNRTVRKDLS
VSSDDSRRTESTPAGSETKDIRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008773
ORF Size 1119 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008773.1
RefSeq Size 3011 bp
RefSeq ORF 1122 bp
Locus ID 18442
UniProt ID P35383
Cytogenetics 7 E2
MW 42.2 kDa
Summary Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity range is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008773" in other vectors (4)
SKU Description Size Price
MC203748 P2ry2 (untagged) - Mouse purinergic receptor P2Y, G-protein coupled 2 (P2ry2), (10ug) 10 ug
$457.00
MG205778 P2ry2 (tGFP-tagged) - Mouse purinergic receptor P2Y, G-protein coupled 2 (P2ry2) 10 ug
$489.00 $657.00
MR205778L3 Lenti ORF clone of P2ry2 (Myc-DDK-tagged) - Mouse purinergic receptor P2Y, G-protein coupled 2 (P2ry2) 10 ug
$757.00
MR205778L4 Lenti ORF clone of P2ry2 (mGFP-tagged) - Mouse purinergic receptor P2Y, G-protein coupled 2 (P2ry2) 10 ug
$757.00

Plasmid Map for MR205778

Download sequence for

Cloning scheme for MR205778

Circular map for MR205778

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