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Setd7 (NM_080793) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205628
Setd7 (Myc-DDK-tagged) - Mouse SET domain containing (lysine methyltransferase) 7 (Setd7)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Setd7
Synonyms 1600028F23Rik; H3K4MT; KMT7; mKIAA1717; Set7; Set7/9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205628 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATAGCGACGATGAGGTGGTGGAGGAAGCCGTGGAAGGGCACCTGGACGATGACGGATTACCACACG
GGTTCTGCACTGTCACCTACTCCTCCACGGACAGATTTGAGGGCAACTTTGTGCATGGAGAAAAGAATGG
GCGTGGGAAATTCTTCTTCTTTGACGGCAGCACCCTGGAGGGATATTACGTGGACGATGCCCTGCAGGGC
CAGGGCGTTTACACCTATGTGGATGGAGGTGTTCTCCAAGGCACCTATGTGGATGGAGAGCTGAATGGAC
CGGCCCAGGAGTACGACTCTGACGGGAGGCTGATCTTCAAGGGACAGTACAAAGACAACAATCGACACGG
AGTGTGTTGGATCCATTACCCGGATGGAGGCAGCCTCGTCGGGGAAGTCAATGAAGATGGAGAGATGACG
GGAGAAAAGATCGCCTATGTGTACCCTGACCAGAGGACTGCGCTCTATGGGAAGTTCATTGACGGAGAGA
TGCTCGAAGGGAAACTGGCCACCCTCATGGCCACCGAAGAAGGGAGGCCACACTTTGAAGTGACGTCGGG
AAGTTCCGTGTACCACTTTGATAAATCGACTTCCTCCTGCATCTCCAGCGATGCTCTCCTTCCAGACCCT
TATGAGTCTGAGAGGGTTTATGTTGCCGACTCTCTCATCTCCAGTGCTGGAGAAGGGCTGTTTTCCAAGG
TAGCAGTTGGACCTAATACTGTTATGTCGTTTTATAATGGAGTCCGAATTACACACCAAGAGGTTGACAG
CAGGGATTGGGCCCTAAATGGGAACACTCTCTCCCTGGACGAGGAGACAGTCATTGATGTGCCCGAGCCC
TACAACCACGTGTCCAAGTACTGTGCCTCCTTGGGACACAAGGCCAATCACTCCTTCACTCCGAACTGCG
TCTATGACCTGTTTGTCCACCCCCGTTTCGGGCCGATCAAGTGCATCCGCACCCTGCGAGCTGTGGAGGC
CGAGGAAGAGCTGACCGTTGCCTACGGCTATGACCACAGCCCCCCGGGGAAGAGTGGGCCTGAAGCCCCC
GAGTGGTATCAAGTGGAGCTGAAGGCCTTCCAGGCCACCCAGCAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205628 protein sequence
Red=Cloning site Green=Tags(s)

MDSDDEVVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQG
QGVYTYVDGGVLQGTYVDGELNGPAQEYDSDGRLIFKGQYKDNNRHGVCWIHYPDGGSLVGEVNEDGEMT
GEKIAYVYPDQRTALYGKFIDGEMLEGKLATLMATEEGRPHFEVTSGSSVYHFDKSTSSCISSDALLPDP
YESERVYVADSLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEP
YNHVSKYCASLGHKANHSFTPNCVYDLFVHPRFGPIKCIRTLRAVEAEEELTVAYGYDHSPPGKSGPEAP
EWYQVELKAFQATQQK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080793
ORF Size 1098 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080793.2
RefSeq Size 7356 bp
RefSeq ORF 1101 bp
Locus ID 73251
UniProt ID Q8VHL1
Cytogenetics 3 C
MW 40.5 kDa
Summary Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the KR-STA-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.UniProtKB/Swiss-Prot Function
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"NM_080793" in other vectors (3)
SKU Description Size Price
MG205628 Setd7 (tGFP-tagged) - Mouse SET domain containing (lysine methyltransferase) 7 (Setd7) 10 ug
$657.00
MR205628L3 Lenti ORF clone of Setd7 (Myc-DDK-tagged) - Mouse SET domain containing (lysine methyltransferase) 7 (Setd7) 10 ug
$757.00
MR205628L4 Lenti ORF clone of Setd7 (mGFP-tagged) - Mouse SET domain containing (lysine methyltransferase) 7 (Setd7) 10 ug
$757.00

Plasmid Map for MR205628

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205628
Circular map for MR205628

file-alt Citations

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