Tram1l1 (NM_146140) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205569
Tram1l1 (Myc-DDK-tagged) - Mouse translocation associated membrane protein 1-like 1 (Tram1l1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tram1l1
Synonyms A830091N21Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205569 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCTCCGCAAGAAGAACGCCAGGAACCCCCCGGTGTTGAGCCACGAATTCATGGTGCAGAACCACG
CGGATATGGTCTCCTGCGTGGGCATGTTCTTCGTGCTGGGGCTTATGTTCGAGGGCACCTCCGAGATGTC
GATCGCGTTCCTCACTCTGCAGCATGGAGTCGTTGTCCCAGCGGAAGGGCTGCCCTCCGGGTCCAGGACC
CTGTACCATTATGGGGTCAAAGATCTGGCCACAGTGTTCTTCTACATGCTGGTGGCCATCATCATCCACG
CCACCATTCAGGAGTACGTGCTAGATAAGCTCAGCAGGAGACTGCAGCTCACCAAAGGCAAACAAAACAA
ACTGAATGAGGCCGGGCAGCTGAGTGTGTTCTATATAGTGTCCGGGATCTGGGGCATGATCATTCTGGCC
TCTGAGAACTGCCTGTCAGACCCTACACTCTTGTGGAAGTCTCAGCCCCACAACATGATGACATTTCAGA
TGAAATTTTTCTACATTTCACAGTTGGCTTACTGGTTTCATAGCTTTCCGGAGCTTTACTTCCAGAAAGT
CAGGAAGCAAGATATCCCGGGTCAGCTCATCTACATTGGCCTCCACCTCTTCCACATTGGAGGGGCCTAT
CTCTTGTACTTGAACCACTTGGGCCTGCTGCTGCTGATGCTGCACTACGCCGTAGAGCTCCTCTCCAGCG
TGTGCAGCCTGCTTTACTTTGGTGATGAGCGGTACCAGAAAGGGTTGTCTCTGTGGCCTATCGTGTTTAT
ATCCGGGAGACTCGTGACATTGATTGTCTCGGTGGTTACAGTAGGGCTTCACCTGGCCGGGACAAATCGG
AATGGCAATGCACTCTCTGGTAATGTCAATGTGCTGGCAGCTAAAATCGCTGTTCTGTCCTCGAGTTGCA
GTATCCAGGTGTACATAACATGGACCTTGACTACTGTCTGGCTTCAGAGATGGTTAGAAGACGCGAACCT
TCATGTTTGTGGAAGGAAGAGACGGTCCAGGGCGAGAAAAGGCACAGAAAATGGAGTGGAGAATCCGAAT
AGAATAGACTCTCCACCAAAGAAGAAAGAGAAGGCTCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205569 protein sequence
Red=Cloning site Green=Tags(s)

MGLRKKNARNPPVLSHEFMVQNHADMVSCVGMFFVLGLMFEGTSEMSIAFLTLQHGVVVPAEGLPSGSRT
LYHYGVKDLATVFFYMLVAIIIHATIQEYVLDKLSRRLQLTKGKQNKLNEAGQLSVFYIVSGIWGMIILA
SENCLSDPTLLWKSQPHNMMTFQMKFFYISQLAYWFHSFPELYFQKVRKQDIPGQLIYIGLHLFHIGGAY
LLYLNHLGLLLLMLHYAVELLSSVCSLLYFGDERYQKGLSLWPIVFISGRLVTLIVSVVTVGLHLAGTNR
NGNALSGNVNVLAAKIAVLSSSCSIQVYITWTLTTVWLQRWLEDANLHVCGRKRRSRARKGTENGVENPN
RIDSPPKKKEKAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146140
ORF Size 1089 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146140.4
RefSeq Size 2225 bp
RefSeq ORF 1092 bp
Locus ID 229801
UniProt ID Q8QZR0
Cytogenetics 3 G1
MW 41 kDa
Summary Stimulatory or required for the translocation of secretory proteins across the ER membrane.UniProtKB/Swiss-Prot Function
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Resources
"NM_146140" in other vectors (4)
SKU Description Size Price
MC201946 Tram1l1 (untagged) - Mouse translocation associated membrane protein 1-like 1 (Tram1l1), (10ug) 10 ug
$457.00
MG205569 Tram1l1 (tGFP-tagged) - Mouse translocation associated membrane protein 1-like 1 (Tram1l1) 10 ug
$657.00
MR205569L3 Lenti ORF clone of Tram1l1 (Myc-DDK-tagged) - Mouse translocation associated membrane protein 1-like 1 (Tram1l1) 10 ug
$757.00
MR205569L4 Lenti ORF clone of Tram1l1 (mGFP-tagged) - Mouse translocation associated membrane protein 1-like 1 (Tram1l1) 10 ug
$757.00

Plasmid Map for MR205569

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Cloning scheme for MR205569

Circular map for MR205569

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