Tob1 (NM_009427) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205562
Tob1 (Myc-DDK-tagged) - Mouse transducer of ErbB-2.1 (Tob1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tob1
Synonyms Tob; Trob
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205562 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCTTGAAATCCAAGTAGCACTCAATTTTATTATTTCTTACTTGTACAATAAGCTTCCCAGGAGAC
GTGTCAACATTTTTGGTGAAGAGCTTGAAAGACTTCTTAAGAAGAAATATGAAGGGCACTGGTATCCTGA
AAAGCCATACAAAGGCTCAGGGTTTAGATGTATACACGTGGGGGAGAAGGTAGACCCCGTGATCGAGCAG
GCATCCAAAGAGAGTGGTTTGGACATTGACGATGTTCGTGGCAATCTGCCACAGGATTTGAGTGTCTGGA
TCGACCCGTTTGAGGTTTCCTACCAGATCGGCGAAAAGGGACCAGTGAAGGTGCTGTACGTGGACGACAG
TAGTGAAACCGGCTGTGAGCTGGATAAGGAGATCAAAAACAGCTTTAACCCGGAGGCCCAGGTGTTCATG
CCCATAAGCGACCCGGCCTCCTCCGTGTCCAGCTCTCCCTCACCTCCCTTCGGCCACTCTGCTGCTGTCA
GCCCTACCTTCATGCCCCGGTCCACGCAGCCTTTAACCTTTACCACTGCCACTTTTGCTGCCACCAAGTT
CGGCTCCACCAAAATGAAGAATAGTGGCCGTAGCAGCAAGGTAGCTCGCACTTCTCCGATCAACCTGGGC
CTGACTGTAAATGTGAACGACCTCCTGAAGCAGAAAGCCATCTCTTCCTCAGTGCACTCTCTGTATGGGC
TGGGCCTGGGCAGCCAGCAGCAGCCACAGCCGCAGCCACAGCAGCAGCAGCAGCAGCAGCCGTCATCATC
CCAGCCGCCGCCTCCACTACCGCAGCAGCAGCAGCAGCAGCCACAGCAGCAGCAGCAGCAGCAGCAGCAA
ACCTCTGCTCTTTCTCCCAATGCCAAGGAATTTATTTTTCCTAACATGCAGGGTCAAGGTAGTAGTACCA
ATGGAATGTTCCCAGGTGACAGCCCCCTCAACCTCAGTCCCCTCCAGTACAGCAATGCCTTTGATGTGTT
TGCGGCCTACGGAGGCCTCAACGAGAAGTCTTTTGTAGACGGCTTGAATTTTAGCTTAAATAACATGCAG
TATTCTAACCAGCAATTCCAGCCTGTTATGGCTAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205562 protein sequence
Red=Cloning site Green=Tags(s)

MQLEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPEKPYKGSGFRCIHVGEKVDPVIEQ
ASKESGLDIDDVRGNLPQDLSVWIDPFEVSYQIGEKGPVKVLYVDDSSETGCELDKEIKNSFNPEAQVFM
PISDPASSVSSSPSPPFGHSAAVSPTFMPRSTQPLTFTTATFAATKFGSTKMKNSGRSSKVARTSPINLG
LTVNVNDLLKQKAISSSVHSLYGLGLGSQQQPQPQPQQQQQQQPSSSQPPPPLPQQQQQQPQQQQQQQQQ
TSALSPNAKEFIFPNMQGQGSSTNGMFPGDSPLNLSPLQYSNAFDVFAAYGGLNEKSFVDGLNFSLNNMQ
YSNQQFQPVMAN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009427
ORF Size 1086 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009427.2, NP_033453.2
RefSeq Size 2288 bp
RefSeq ORF 1089 bp
Locus ID 22057
UniProt ID Q61471
Cytogenetics 11 D
MW 40.1 kDa
Summary Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_009427" in other vectors (5)
SKU Description Size Price
MG205562 Tob1 (tGFP-tagged) - Mouse transducer of ErbB-2.1 (Tob1) 10 ug
$657.00
MR205562L1 Lenti ORF clone of Tob1 (Myc-DDK-tagged) - Mouse transducer of ErbB-2.1 (Tob1) 10 ug
$757.00
MR205562L2 Lenti ORF clone of Tob1 (mGFP-tagged) - Mouse transducer of ErbB-2.1 (Tob1) 10 ug
$757.00
MR205562L3 Lenti ORF clone of Tob1 (Myc-DDK-tagged) - Mouse transducer of ErbB-2.1 (Tob1) 10 ug
$757.00
MR205562L4 Lenti ORF clone of Tob1 (mGFP-tagged) - Mouse transducer of ErbB-2.1 (Tob1) 10 ug
$757.00

Plasmid Map for MR205562

Download sequence for

Cloning scheme for MR205562

Circular map for MR205562

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products