Adk (NM_134079) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205531
Adk (Myc-DDK-tagged) - Mouse adenosine kinase (Adk)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adk
Synonyms 2310026J05Rik; 5033405D03Rik; AI255373; AI987814; Ak
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205531 representing NM_134079
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCTGCGGACGAGCCCAAGCCCAAAAAGCTCAAGGTGGAAGCGCCACAAGCGCTGAGTGAAAATG
TGCTATTTGGAATGGGGAATCCTCTTCTTGACATCTCTGCTGTAGTAGACAAAGATTTCCTTGATAAGTA
TTCTCTGAAACCAAATGACCAGATCTTGGCTGAAGACAAGCACAAGGAACTGTTTGATGAACTTGTGAAA
AAATTCAAAGTTGAATATCATGCTGGTGGCTCTACGCAGAATTCAATGAAAGTGGCTCAGTGGTTGATTC
AGGAGCCACACAAAGCAGCAACATTCTTTGGATGCATTGGGATAGATAAGTTTGGGGAGATCCTGAAGCG
TAAGGCTGCTGACGCACATGTGGATGCTCATTACTATGAGCAGAACGAGCAGCCAACAGGAACTTGTGCT
GCGTGCATCACTGGTGGCAACAGGTCCCTCGTTGCTAACCTTGCTGCCGCCAATTGTTACAAGAAAGAGA
AGCACCTTGACCTGGAGCGGAACTGGGTGTTGGTAGAGAAAGCCAGAGTTTACTACATAGCTGGCTTTTT
TCTCACAGTCTCCCCAGAGTCGGTATTGAAAGTGGCTCGCTATGCTGCCGAGAACAACAGGGTCTTCACT
TTGAACCTGTCTGCACCGTTCATTAGCCAGTTCTTCAAGGAAGCCTTGATGGACGTCATGCCTTATGTTG
ATATCCTCTTTGGAAATGAGACGGAAGCTGCCACTTTTGCTAGAGAGCAAGGCTTTGAGACTAAAGACAT
TAAAGAAATAGCCAAAAAGGCGCAGGCTCTTCCAAAGGTGAACTCGAAGAGGCAGAGGACCGTGATCTTC
ACACAAGGGCGAGATGACACCATAGTGGCTGCAGAGAATGATGTCACTGCTTTTCCTGTCTTGGATCAAA
ACCAGGAAGAGATCATTGACACCAATGGAGCTGGAGATGCATTTGTTGGAGGCTTTCTGTCTCAACTGGT
CTCTGACAAGCCTCTGACTGAGTGCATTCGCGCTGGCCACTACGCAGCAAGCGTCATCATTAGACGAACT
GGCTGTACCTTTCCCGAGAAGCCAGACTTCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205531 representing NM_134079
Red=Cloning site Green=Tags(s)

MAAADEPKPKKLKVEAPQALSENVLFGMGNPLLDISAVVDKDFLDKYSLKPNDQILAEDKHKELFDELVK
KFKVEYHAGGSTQNSMKVAQWLIQEPHKAATFFGCIGIDKFGEILKRKAADAHVDAHYYEQNEQPTGTCA
ACITGGNRSLVANLAAANCYKKEKHLDLERNWVLVEKARVYYIAGFFLTVSPESVLKVARYAAENNRVFT
LNLSAPFISQFFKEALMDVMPYVDILFGNETEAATFAREQGFETKDIKEIAKKAQALPKVNSKRQRTVIF
TQGRDDTIVAAENDVTAFPVLDQNQEEIIDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASVIIRRT
GCTFPEKPDFH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_134079
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134079.2
RefSeq Size 1737 bp
RefSeq ORF 1086 bp
Locus ID 11534
UniProt ID P55264
Cytogenetics 14 A3
MW 40.6 kDa
Summary ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_134079" in other vectors (4)
SKU Description Size Price
MC207219 Adk (untagged) - Mouse adenosine kinase (Adk), (10ug) 10 ug
$457.00
MG205531 Adk (tGFP-tagged) - Mouse adenosine kinase (Adk) 10 ug
$657.00
MR205531L3 Lenti ORF clone of Adk (Myc-DDK-tagged) - Mouse adenosine kinase (Adk) 10 ug
$757.00
MR205531L4 Lenti ORF clone of Adk (mGFP-tagged) - Mouse adenosine kinase (Adk) 10 ug
$757.00

Plasmid Map for MR205531

Download sequence for

Cloning scheme for MR205531

Circular map for MR205531

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