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Ffar4 (NM_181748) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR205527L3
Lenti ORF clone of Ffar4 (Myc-DDK-tagged) - Mouse omega-3 fatty acid receptor 1 (O3far1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $757.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ffar4
Synonyms AI552415; Gpr120; Gpr129; GT01; KPG_013; O3far1; Pgr4
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR205527).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181748
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181748.2
RefSeq Size 1393 bp
RefSeq ORF 1086 bp
Locus ID 107221
UniProt ID Q7TMA4
Cytogenetics 19 C2
Summary Receptor for medium and long-chain free fatty acids (FFAs). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti-inflammatory effects, particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1-dependent effect, but independent of the G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. Mediates the taste of fatty acids. Mediates FFA-induced inhibition of apoptosis in enteroendocrine cells. May play a role in the regulation of adipocyte development and differentiation.UniProtKB/Swiss-Prot Function
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"NM_181748" in other vectors (4)
SKU Description Size Price
MC202355 Ffar4 (untagged) - Mouse omega-3 fatty acid receptor 1 (O3far1), (10ug) 10 ug
$457.00
MG205527 Ffar4 (tGFP-tagged) - Mouse G protein-coupled receptor 120 (Gpr120) 10 ug
$489.00 $657.00
MR205527 Ffar4 (Myc-DDK-tagged) - Mouse omega-3 fatty acid receptor 1 (O3far1) 10 ug
$289.00 $457.00
MR205527L4 Lenti ORF clone of Ffar4 (mGFP-tagged) - Mouse omega-3 fatty acid receptor 1 (O3far1) 10 ug
$757.00

Plasmid Map for MR205527L3

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Cloning scheme for MR205527L3
Circular map for MR205527L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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