Crlf2 (NM_016715) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205490
Crlf2 (Myc-DDK-tagged) - Mouse cytokine receptor-like factor 2 (Crlf2), transcript variant 2
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Crlf2
Synonyms CRLM2; Ly114; Tpte2; Tslpr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205490 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATGGGCACTCGCGGTCATCCTCCTGCCTCGGCTCCTTGCGGCGGCAGCGGCGGCGGCGGCGGTGA
CGTCACGGGGTGATGTCACAGTCGTCTGCCATGACCTGGAGACGGTGGAGGTCACGTGGGGCTCGGGCCC
CGACCACCACAGCGCCAACTTGAGCCTGGAGTTCCGTTATGGTACTGGCGCCCTGCAACCCTGCCCGCGA
TATTTCCTGTCCGGCGCTGGTGTCACTTCCGGGTGCATCCTCCCCGCGGCGAGGGCGGGGCTGCTGGAGC
TGGCACTGCGCGACGGAGGCGGGGCCATGGTGTTTAAGGCTAGGCAGCGCGCGTCCGCCTGGCTGAAGCC
CCGCCCACCTTGGAATGTGACGCTGCTCTGGACACCAGACGGGGACGTGACTGTCTCCTGGCCTGCCCAC
TCCTACCTGGGCCTGGACTACGAGGTGCAGCACCGGGAGAGCAATGACGATGAGGACGCCTGGCAGACGA
CCTCAGGGCCCTGCTGTGACTTGACAGTGGGCGGGCTCGACCCCGCGCGCTGCTATGACTTCCGGGTTCG
GGCGTCGCCCCGGGCCGCGCACTATGGCCTGGAGGCGCAGCCTAGCGAGTGGACAGCGGTGACAAGGCTT
TCCGGGGCAGCATCCGCGGCCTCCTGTACCGCAAGCCCCGCCCCATCCCCGGCCCTGGCCCCGCCCCTCC
TGCCCCTGGGCTGCGGCCTAGCAGCGCTGCTGACACTGTCCCTGCTCCTGGCCGCCCTGAGGCTTCGCAG
GGTGAAAGATGCGCTGCTGCCCTGCGTCCCTGACCCCAGCGGCTCCTTCCCTGGACTCTTTGAGAAGCAT
CACGGGAACTTCCAGGCCTGGATTGCGGACGCCCAGGCCACAGCCCCGCCAGCCAGGACCGAGGAGGAAG
ATGACCTCATCCACCCCAAGGCTAAGAGGGTGGAGCCCGAGGATGGCACCTCCCTCTGCACCGTGCCAAG
GCCACCCAGCTTCGAGCCAAGGGGGCCGGGAGGCGGGGCCATGGTGTCAGTGGGCGGGGCCACGTTCATG
GTGGGCGACAGCGGCTACATGACCCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205490 protein sequence
Red=Cloning site Green=Tags(s)

MAWALAVILLPRLLAAAAAAAAVTSRGDVTVVCHDLETVEVTWGSGPDHHSANLSLEFRYGTGALQPCPR
YFLSGAGVTSGCILPAARAGLLELALRDGGGAMVFKARQRASAWLKPRPPWNVTLLWTPDGDVTVSWPAH
SYLGLDYEVQHRESNDDEDAWQTTSGPCCDLTVGGLDPARCYDFRVRASPRAAHYGLEAQPSEWTAVTRL
SGAASAASCTASPAPSPALAPPLLPLGCGLAALLTLSLLLAALRLRRVKDALLPCVPDPSGSFPGLFEKH
HGNFQAWIADAQATAPPARTEEEDDLIHPKAKRVEPEDGTSLCTVPRPPSFEPRGPGGGAMVSVGGATFM
VGDSGYMTL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016715
ORF Size 1077 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016715.3
RefSeq Size 1338 bp
RefSeq ORF 1080 bp
Locus ID 57914
UniProt ID Q8CII9
Cytogenetics 5 F
MW 37.8 kDa
Summary Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016715" in other vectors (5)
SKU Description Size Price
MG205490 Crlf2 (tGFP-tagged) - Mouse transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 (Tpte2) 10 ug
$657.00
MR205490L1 Lenti ORF clone of Crlf2 (Myc-DDK-tagged) - Mouse cytokine receptor-like factor 2 (Crlf2), transcript variant 2 10 ug
$757.00
MR205490L2 Lenti ORF clone of Crlf2 (mGFP-tagged) - Mouse cytokine receptor-like factor 2 (Crlf2), transcript variant 2 10 ug
$757.00
MR205490L3 Lenti ORF clone of Crlf2 (Myc-DDK-tagged) - Mouse cytokine receptor-like factor 2 (Crlf2), transcript variant 2 10 ug
$757.00
MR205490L4 Lenti ORF clone of Crlf2 (mGFP-tagged) - Mouse cytokine receptor-like factor 2 (Crlf2), transcript variant 2 10 ug
$757.00

Plasmid Map for MR205490

Download sequence for

Cloning scheme for MR205490

Circular map for MR205490

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