Gnaq (NM_008139) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205488
Gnaq (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha q polypeptide (Gnaq)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gnaq
Synonyms 1110005L02Rik; 6230401I02Rik; AA408290; AW060788; Dsk1; Dsk10; Galphaq; Gq; GqI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205488 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTCTGGAGTCCATCATGGCGTGCTGCCTGAGCGAGGAGGCCAAGGAAGCCCGGAGGATCAACGACG
AGATCGAGCGGCAGCTGCGCAGGGACAAGCGCGACGCCCGCCGGGAGCTCAAGCTGCTGCTGCTGGGGAC
AGGGGAGAGTGGCAAGAGCACCTTCATCAAGCAGATGAGGATCCACGGGTCGGGCTACTCTGACGAAGAC
AAGCGCGGCTTCACCAAGCTGGTGTATCAGAACATCTTCACGGCCATGCAGGCCATGATCAGAGCGATGG
ACACGCTCAAGATCCCATACAAGTATGAACACAATAAGGCTCATGCACAATTGGTTCGAGAGGTTGATGT
GGAGAAGGTGTCTGCTTTTGAGAATCCATATGTAGATGCAATAAAGAGCTTGTGGAATGATCCTGGAATC
CAGGAGTGCTACGACAGACGACGGGAATATCAGTTATCTGACTCTACCAAATACTATCTGAATGACTTGG
ACCGTGTAGCCGACCCTTCCTATCTGCCTACACAACAAGACGTGCTTAGAGTTCGAGTCCCCACTACAGG
GATCATCGAATACCCCTTTGACTTACAAAGTGTCATTTTCAGAATGGTCGATGTAGGGGGCCAAAGGTCA
GAGAGAAGAAAATGGATACACTGCTTTGAAAATGTCACCTCCATCATGTTTCTAGTAGCGCTTAGCGAAT
ATGATCAAGTTCTTGTGGAGTCAGACAATGAGAACCGCATGGAGGAGAGCAAAGCACTCTTTAGAACAAT
TATCACCTACCCCTGGTTCCAGAACTCCTCTGTGATTCTGTTCTTAAACAAGAAAGATCTTCTAGAGGAG
AAAATCATGTATTCCCACCTAGTCGACTACTTCCCAGAATATGATGGACCCCAGAGAGATGCCCAGGCAG
CTCGAGAATTCATCCTGAAAATGTTCGTGGACCTGAACCCCGACAGTGACAAAATCATCTACTCCCACTT
CACGTGCGCCACAGATACCGAGAACATCCGCTTCGTCTTTGCAGCCGTCAAGGACACCATCCTGCAGCTG
AACCTGAAGGAGTACAATCTGGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205488 protein sequence
Red=Cloning site Green=Tags(s)

MTLESIMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIHGSGYSDED
KRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFENPYVDAIKSLWNDPGI
QECYDRRREYQLSDSTKYYLNDLDRVADPSYLPTQQDVLRVRVPTTGIIEYPFDLQSVIFRMVDVGGQRS
ERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEE
KIMYSHLVDYFPEYDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQL
NLKEYNLV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008139
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008139.2
RefSeq Size 5634 bp
RefSeq ORF 1080 bp
Locus ID 14682
UniProt ID P21279
Cytogenetics 19 11.01 cM
MW 42 kDa
Summary Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008139" in other vectors (4)
SKU Description Size Price
MC207290 Gnaq (untagged) - Mouse guanine nucleotide binding protein, alpha q polypeptide (Gnaq), (10ug) 10 ug
$503.00
MG205488 Gnaq (tGFP-tagged) - Mouse guanine nucleotide binding protein, alpha q polypeptide (Gnaq) 10 ug
$489.00 $657.00
MR205488L3 Lenti ORF clone of Gnaq (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha q polypeptide (Gnaq) 10 ug
$757.00
MR205488L4 Lenti ORF clone of Gnaq (mGFP-tagged) - Mouse guanine nucleotide binding protein, alpha q polypeptide (Gnaq) 10 ug
$757.00

Plasmid Map for MR205488

Download sequence for

Cloning scheme for MR205488

Circular map for MR205488

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