G6pc (NM_008061) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205458
G6pc (Myc-DDK-tagged) - Mouse glucose-6-phosphatase, catalytic (G6pc)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol G6pc
Synonyms AW107337; G6P; G6Pase; G6pc1; G6pt; Glc-6-; Glc-6-Pase
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205458 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAAGGAATGAACATTCTCCATGACTTTGGGATCCAGTCGACTCGCTATCTCCAAGTGAATTACC
AAGACTCCCAGGACTGGTTCATCCTTGTGTCTGTGATTGCTGACCTGAGGAACGCCTTCTATGTCCTCTT
TCCCATCTGGTTCCATCTTAAAGAGACTGTGGGCATCAATCTCCTCTGGGTGGCAGTGGTCGGAGACTGG
TTCAACCTCGTCTTCAAGTGGATTCTGTTTGGACAACGCCCGTATTGGTGGGTCCTGGACACCGACTACT
ACAGCAACAGCTCCGTGCCTATAATAAAGCAGTTCCCTGTCACCTGTGAGACCGGACCAGGAAGTCCCTC
TGGCCATGCCATGGGCGCAGCAGGTGTATACTATGTTATGGTCACTTCTACTCTTGCTATCTTTCGAGGA
AAGAAAAAGCCAACGTATGGATTCCGGTGTTTGAACGTCATCTTGTGGTTGGGATTCTGGGCTGTGCAGC
TGAACGTCTGTCTGTCCCGGATCTACCTTGCTGCTCACTTTCCCCACCAGGTCGTGGCTGGAGTCTTGTC
AGGCATTGCTGTGGCTGAAACTTTCAGCCACATCCGGGGCATCTACAATGCCAGCCTCCGGAAGTATTGT
CTCATCACCATCTTCTTGTTTGGTTTCGCGCTTGGATTCTACCTGCTACTAAAAGGGCTAGGGGTGGACC
TCCTGTGGACTTTGGAGAAAGCTAAGAGATGGTGTGAGCGGCCAGAATGGGTCCACCTTGACACTACACC
CTTTGCCAGCCTCTTCAAAAACCTGGGAACCCTCTTGGGGTTGGGGCTGGCCCTCAACTCCAGCATGTAC
CGGAAGAGCTGCAAGGGAGAACTCAGCAAGTTGCTCCCATTCCGCTTCGCCTGCATTGTGGCTTCCTTGG
TCCTCCTGCATCTCTTTGACTCTCTGAAGCCCCCATCCCAGGTTGAGTTGATCTTCTACATCTTGTCTTT
CTGCAAGAGCGCAACAGTTCCCTTTGCATCTGTCAGTCTTATCCCATACTGCCTAGCCCGGATCCTGGGA
CAGACACACAAGAAGTCTTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205458 protein sequence
Red=Cloning site Green=Tags(s)

MEEGMNILHDFGIQSTRYLQVNYQDSQDWFILVSVIADLRNAFYVLFPIWFHLKETVGINLLWVAVVGDW
FNLVFKWILFGQRPYWWVLDTDYYSNSSVPIIKQFPVTCETGPGSPSGHAMGAAGVYYVMVTSTLAIFRG
KKKPTYGFRCLNVILWLGFWAVQLNVCLSRIYLAAHFPHQVVAGVLSGIAVAETFSHIRGIYNASLRKYC
LITIFLFGFALGFYLLLKGLGVDLLWTLEKAKRWCERPEWVHLDTTPFASLFKNLGTLLGLGLALNSSMY
RKSCKGELSKLLPFRFACIVASLVLLHLFDSLKPPSQVELIFYILSFCKSATVPFASVSLIPYCLARILG
QTHKKSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008061
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008061.4
RefSeq Size 2245 bp
RefSeq ORF 1074 bp
Locus ID 14377
UniProt ID P35576
Cytogenetics 11 D
MW 40.5 kDa
Summary The enzyme encoded by this gene is a multisubunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for glucose-6-phosphate, inorganic phosphate, and glucose. This gene is one of three glucose-6-phosphatase catalytic-subunit-encoding genes in mouse. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). provided by RefSeq, Sep 2015
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Documents
Resources
"NM_008061" in other vectors (3)
SKU Description Size Price
MC201182 G6pc (untagged) - Mouse glucose-6-phosphatase, catalytic (G6pc), (10ug) 10 ug
$457.00
MR205458L3 Lenti ORF clone of G6pc (Myc-DDK-tagged) - Mouse glucose-6-phosphatase, catalytic (G6pc) 10 ug
$757.00
MR205458L4 Lenti ORF clone of G6pc (mGFP-tagged) - Mouse glucose-6-phosphatase, catalytic (G6pc) 10 ug
$757.00

Plasmid Map for MR205458

Download sequence for

Cloning scheme for MR205458

Circular map for MR205458

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