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C130050O18Rik (NM_177000) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205440
C130050O18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C130050O18 gene (C130050O18Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol C130050O18Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205440 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAGAGAGCATGCCCCAGCCTCACGGCAACCCCCCAGTGCTGACAGAGCGTTGTGTGGATTTCAGG
CTGGCACAGGACTGAGAGCTCTCCTGAAGATGGACCGTCACAACATGTCCTGGGTCTGCTACCCGAGCCA
TGTGTCCCCGGCTGTGGAGGAAGTCATTGCAGCACAGAGCATCATTTCTAGGTGCACGCAGGTGTACATA
GCAGTGTGCGTCCCACTGAGCCTAGTGATGGGGCTCTTCAGTCTGAGCGTGTTCATCTTAGACCGTACTA
GGCTGGGGGTGCTGGACAGGCTCCTTGCAGGCCTCACAGCCACTAGTATCCTGGTGACTCTACTGTCCCT
CAATGCTGCTGTCCGACCCGACTACATGTCCACGACAAACCTGGGCTGTGGCGCACTCTCATTCTTCTCC
AATGTCTGCTACTTCATGGCCCAGTACCTGCAGGGAGCCACGCTTCTCCTGTCCTTTCTGCCGGAATCAC
CCTGCTGCCGGCTCTTGGCAAGGCCTATGGCAAGCCTAGCTGCCATTGGGGGCTGTGCGGTCTGTAGCTC
CCTGATTGTGGTGTCCCTGCTGGGCACATCTGGGGAGTTGCACACAACCACCATGTGCCAGGCGGATCCG
CTGACTGCATGGCCCGAATATGAGATCGTCAAGTTCAGCCTGGGCTTTGCTCTTGCCCTGAGCTTCCAGA
TGGTTCTATTGCTCCTGTATGCTACACAGCCAGTCTGGCGAGCAGCTCCTTCTCAAAGGGACACAGCCTC
GGGGCGCTGGGTGGTACTGGCTGTGGCTCTCAACATGCTTGCCTGTAGACTCTTCTTCAATGTGGTCCTC
CTGCATCGGGCCCGCCTGAAGCTGTGGAAGGATGTGGGCTCCCCCTGGGATGAACTGCTCATGAACCTCG
CAGAGCTGGTCCTGTCTGGAGAGAGCTGCATCAACGTGGTGGCCATCCTCCTCCTCCACACACCATGCAG
GCTCAGGCTGCTTGGCCTCCTTGAGCGCCTCACACAGAGATGCAGGCCAGGGCTGGACAACAGCATGTCC
TTGAACAGAGTAGGGGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205440 protein sequence
Red=Cloning site Green=Tags(s)

MNREHAPASRQPPSADRALCGFQAGTGLRALLKMDRHNMSWVCYPSHVSPAVEEVIAAQSIISRCTQVYI
AVCVPLSLVMGLFSLSVFILDRTRLGVLDRLLAGLTATSILVTLLSLNAAVRPDYMSTTNLGCGALSFFS
NVCYFMAQYLQGATLLLSFLPESPCCRLLARPMASLAAIGGCAVCSSLIVVSLLGTSGELHTTTMCQADP
LTAWPEYEIVKFSLGFALALSFQMVLLLLYATQPVWRAAPSQRDTASGRWVVLAVALNMLACRLFFNVVL
LHRARLKLWKDVGSPWDELLMNLAELVLSGESCINVVAILLLHTPCRLRLLGLLERLTQRCRPGLDNSMS
LNRVGG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177000
ORF Size 1068 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177000.3, NP_795974.2
RefSeq Size 2006 bp
RefSeq ORF 1071 bp
Locus ID 319772
Cytogenetics 5 G2
MW 38.8 kDa
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"NM_177000" in other vectors (4)
SKU Description Size Price
MC207955 C130050O18Rik (untagged) - Mouse RIKEN cDNA C130050O18 gene (C130050O18Rik), (10ug) 10 ug
$503.00
MG205440 C130050O18Rik (tGFP-tagged) - Mouse RIKEN cDNA C130050O18 gene (C130050O18Rik) 10 ug
$657.00
MR205440L3 Lenti ORF clone of C130050O18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C130050O18 gene (C130050O18Rik) 10 ug
$757.00
MR205440L4 Lenti ORF clone of C130050O18Rik (mGFP-tagged) - Mouse RIKEN cDNA C130050O18 gene (C130050O18Rik) 10 ug
$757.00

Plasmid Map for MR205440

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205440
Circular map for MR205440

file-alt Citations

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