Ptgr2 (NM_029880) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205330
Ptgr2 (Myc-DDK-tagged) - Mouse prostaglandin reductase 2 (Ptgr2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptgr2
Synonyms 1810016I24Rik; 9130222H03Rik; 9630002F03Rik; AI838763; B830026H24Rik; PGR-2; PRG-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205330 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCATACAAAGAGTGGTATTGAATTCCCGACCTGGGAAAAATGGAAATCCAGTCGCAGAGAACTTCA
GGGTGGAAGAGTTCAGTTTACTGGATGCTCTCAATGAAGGTCAAGTTCAAGTGAGGACTCTTTATCTCTC
GGTGGATCCTTACATGCGCTGTAAGATGAACGAGGACACTGGCACTGACTACTTGGCACCGTGGCAGCTG
GCGCAGGTGGCTGATGGTGGAGGAATTGGAATTGTAGAAGAGAGCAAGCACCAGAAGTTGGCTAAAGGCG
ATTTTGTGACTTCGTTTTACTGGCCCTGGCAAACTAAGGCAATTCTAGACGGGAATGGCCTTGAAAAGGT
AGACCCACAACTTGTAGATGGACACCTTTCATATTTTCTTGGAGCTATAGGTATGCCTGGCTTGACTTCC
TTGATTGGGGTACAAGAGAAAGGCCATATATCTGCTGGATCTAATCAGACAATGGTTGTCAGCGGAGCAG
CAGGCGCCTGTGGATCTTTGGCTGGGCAGATTGGCCACCTGCTTGGCTGTTCCAGAGTGGTGGGAATTTG
TGGAACTCAGGAGAAATGTCTCTTTTTGACCTCAGAGCTGGGGTTTGATGCTGCAGTTAATTACAAAACA
GGGAATGTGGCAGAGCAGCTGCGAGAAGCGTGCCCGGGCGGAGTGGATGTCTACTTTGACAATGTTGGAG
GTGACATCAGCAACACGGTGATAAGTCAGATGAATGAGAACAGCCACATCATTCTGTGTGGTCAGATTTC
TCAGTACAATAAAGATGTGCCCTACCCTCCTCCACTGCCCCCTGCAGTAGAAGCCATCCGGAAGGAACGA
AACATCACAAGAGAGAGATTTACGGTATTAAATTATAAAGATAAATTTGAGCCTGGAATTCTACAGCTGA
GTCAGTGGTTTAAAGAAGGAAAGCTAAAGGTCAAGGAGACCGTGGCAAAGGGCTTGGAAAACATGGGAGT
TGCATTCCAGTCCATGATGACAGGGGGCAACGTAGGGAAACAGATCGTCTGCATTTCAGAAGATTCTTCT
CTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205330 protein sequence
Red=Cloning site Green=Tags(s)

MIIQRVVLNSRPGKNGNPVAENFRVEEFSLLDALNEGQVQVRTLYLSVDPYMRCKMNEDTGTDYLAPWQL
AQVADGGGIGIVEESKHQKLAKGDFVTSFYWPWQTKAILDGNGLEKVDPQLVDGHLSYFLGAIGMPGLTS
LIGVQEKGHISAGSNQTMVVSGAAGACGSLAGQIGHLLGCSRVVGICGTQEKCLFLTSELGFDAAVNYKT
GNVAEQLREACPGGVDVYFDNVGGDISNTVISQMNENSHIILCGQISQYNKDVPYPPPLPPAVEAIRKER
NITRERFTVLNYKDKFEPGILQLSQWFKEGKLKVKETVAKGLENMGVAFQSMMTGGNVGKQIVCISEDSS
L

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029880
ORF Size 1053 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 3121 bp
RefSeq ORF 1056 bp
Locus ID 77219
UniProt ID Q8VDQ1
Cytogenetics 12 D1
MW 38.1 kDa
Summary Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029880" in other vectors (4)
SKU Description Size Price
MC204036 Ptgr2 (untagged) - Mouse prostaglandin reductase 2 (Ptgr2), (10ug) 10 ug
$457.00
MG205330 Ptgr2 (tGFP-tagged) - Mouse zinc binding alcohol dehydrogenase, domain containing 1 (Zadh1) 10 ug
$657.00
MR205330L3 Lenti ORF clone of Ptgr2 (Myc-DDK-tagged) - Mouse prostaglandin reductase 2 (Ptgr2) 10 ug
$757.00
MR205330L4 Lenti ORF clone of Ptgr2 (mGFP-tagged) - Mouse prostaglandin reductase 2 (Ptgr2) 10 ug
$757.00

Plasmid Map for MR205330

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Cloning scheme for MR205330

Circular map for MR205330

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