St3gal2 (NM_009179) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205286
St3gal2 (Myc-DDK-tagged) - Mouse ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (St3gal2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol St3gal2
Synonyms AI429591; AW822065; Siat5; ST3GalII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205286 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTGCTCTCTTCGGGTGTGGTTCCTCTCTATGGCATTCCTGCTGGTGTTCATTATGTCCCTGCTCT
TCACCTACTCGCACCACAGCATGGCTACCTTGCCCTACCTGGACTCGGGGGCTCTGGGCGGCACACACCG
GGTGAAGCTGGTACCAGGCTATTCAGGACTACAGCGCCTCGGCAAGGAGGGGCTTTTGGGGAGAAATTGT
GCCTGCAGTCGCTGCATGGGTGACGCCAGCACCTCTGAATGGTTTGACAGCCACTTTGACGGCAACATCT
CACCCGTCTGGACCAGAGATAACATGAACCTCCCCCCGGATGTCCAGCGGTGGTGGATGATGCTACAACC
CCAATTCAAGTCACACAACACCAACGAAGTGCTGGAAAAACTGTTCCAGATCGTGCCTGGCGAGAACCCC
TACCGCTTCCGGGACCCCCAACAGTGCCGGCGCTGTGCTGTGGTCGGGAACTCAGGCAACCTTCGGGGCT
CTGGCTATGGACAAGAAGTGGACAGCCACAACTTTATCATGAGGATGAATCAGGCACCGACTGTGGGCTT
TGAGAAGGATGTTGGCAGCCGAACAACTCACCATTTCATGTACCCAGAGAGTGCCAAGAACCTGCCTGCC
AATGTCAGCTTCGTGCTGGTCCCCTTCAAGGCCCTGGACCTAATGTGGATTGCCAGCGCCCTCTCCACAG
GACAGATCCGATTCACCTATGCCCCAGTGAAGTCCTTCCTTCGAGTGGACAAAGAGAAGGTCCAGATTTA
CAACCCAGCCTTCTTCAAGTACATTCACGACAGGTGGACCGAGCACCACGGGCGCTACCCTTCCACAGGA
ATGCTGGTGCTCTTCTTTGCACTACATGTGTGTGATGAGGTGAACGTGTATGGGTTCGGGGCCGACAGTC
GAGGCAACTGGCACCACTACTGGGAGAACAACCGGTACGCGGGCGAGTTCCGGAAGACAGGCGTGCATGA
CGCCGACTTCGAAGCCCACATCATCGACATGCTGGCCAAGGCCAGCAAGATCGAGGTCTACCGAGGGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205286 protein sequence
Red=Cloning site Green=Tags(s)

MKCSLRVWFLSMAFLLVFIMSLLFTYSHHSMATLPYLDSGALGGTHRVKLVPGYSGLQRLGKEGLLGRNC
ACSRCMGDASTSEWFDSHFDGNISPVWTRDNMNLPPDVQRWWMMLQPQFKSHNTNEVLEKLFQIVPGENP
YRFRDPQQCRRCAVVGNSGNLRGSGYGQEVDSHNFIMRMNQAPTVGFEKDVGSRTTHHFMYPESAKNLPA
NVSFVLVPFKALDLMWIASALSTGQIRFTYAPVKSFLRVDKEKVQIYNPAFFKYIHDRWTEHHGRYPSTG
MLVLFFALHVCDEVNVYGFGADSRGNWHHYWENNRYAGEFRKTGVHDADFEAHIIDMLAKASKIEVYRGN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009179
ORF Size 1050 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009179.3, NP_033205.2
RefSeq Size 4470 bp
RefSeq ORF 1053 bp
Locus ID 20444
UniProt ID Q11204
Cytogenetics 8 E1
MW 40.1 kDa
Summary Responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found in terminal carbohydrate groups of certain glycoproteins, oligosaccharides and glycolipids. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009179" in other vectors (5)
SKU Description Size Price
MC205307 St3gal2 (untagged) - Mouse ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (St3gal2), (10ug) 10 ug
$457.00
MG205303 St3gal2 (tGFP-tagged) - Mouse ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (St3gal2), transcript variant 1 10 ug
$703.00
MR205286L3 Lenti ORF clone of St3gal2 (Myc-DDK-tagged) - Mouse ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (St3gal2) 10 ug
$757.00
MR205286L4 Lenti ORF clone of St3gal2 (mGFP-tagged) - Mouse ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (St3gal2) 10 ug
$757.00
MR205303 St3gal2 (Myc-DDK-tagged) - Mouse ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (St3gal2) 10 ug
$503.00

Plasmid Map for MR205286

Download sequence for

Cloning scheme for MR205286

Circular map for MR205286

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