Pdlim2 (NM_145978) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205282
Pdlim2 (Myc-DDK-tagged) - Mouse PDZ and LIM domain 2 (Pdlim2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdlim2
Synonyms 4732462F18Rik; Slim
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205282 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTGACTGTGGATGTGGCAGGACCAGCACCTTGGGGCTTCCGAATTAGCGGGGGCAGAGATTTCC
ACACACCCATCATTGTGACCAAGGTCACAGAGCGGGGCAAGGCTGAAGCAGCTGATCTCCGGCCTGGCGA
CATCATTGTGGCCATCAATGGACAGAGTGCAGAGAACATGCTACACGCGGAGGCCCAAAGCAAGATCCGA
CAGAGCGCCTCACCCCTAAGACTGCAGCTGGACCGGTCCCAAACAGCCTCTCCTGGGCAGACCAATGGGG
AGGGCTCCTTGGAAGTGCTGGCAACCAGATTCCAGGGCTCCCTGAGGACACACCGTGACAGCCAGTCTTC
CCAGAGGTCTGCCTGCTTCAGCCCAGTCTCTCTCAGCCCCAGGCCTTGCAGCCCCTTCTCCACCCCACCC
CCTACCAGCCCAGTTGCCCTTTCTAAAGAGGATATGATTGGCTGTAGTTTCCAGAGTCTGACACACTCTC
CAGGCCTTGCTGCTGCTCACCACTTGACCTACCCTGGCCACCCCACCAGCCAACAGGCCGGCCACAGCAG
CCCAAGCGACTCCGCAGTGAGGGTGCTGCTCCATTCCCCAGGACGGCCCTCCAGCCCTAGGTTCAGCAGT
TTGGATCTGGAGGAAGACTCAGAGGTGTTCAAGATGCTGCAGGAGAACCGCCAGGGACGGGCCGCCCCAA
GGCAGTCCAGCTCTTTTCGACTCTTACAGGAAGCCTTGGAGGCTGAGGAGAGAGGTGGCACACCTGCCTT
TGTGCCCAGCTCGCTGAGCTCCCAGGCTTCCTTGCCCACCTCCAGGGCCTTGGCCACTCCACCCAAGCTC
CACACCTGTGAGAAATGCAGCGTCAACATCTCGAACCAGGCGGTCCGCATCCAGGAGGGGAGGTACCGAC
ACCCTGGCTGCTACACTTGCGCAGACTGTGGGCTGAACCTGAAGATGCGCGGCCACTTCTGGGTGGGCAA
TGAGTTGTACTGCGAGAAGCATGCCCGCCAGCGCTACTCTATGCCTGGAACTCTCAACTCTCGAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205282 protein sequence
Red=Cloning site Green=Tags(s)

MALTVDVAGPAPWGFRISGGRDFHTPIIVTKVTERGKAEAADLRPGDIIVAINGQSAENMLHAEAQSKIR
QSASPLRLQLDRSQTASPGQTNGEGSLEVLATRFQGSLRTHRDSQSSQRSACFSPVSLSPRPCSPFSTPP
PTSPVALSKEDMIGCSFQSLTHSPGLAAAHHLTYPGHPTSQQAGHSSPSDSAVRVLLHSPGRPSSPRFSS
LDLEEDSEVFKMLQENRQGRAAPRQSSSFRLLQEALEAEERGGTPAFVPSSLSSQASLPTSRALATPPKL
HTCEKCSVNISNQAVRIQEGRYRHPGCYTCADCGLNLKMRGHFWVGNELYCEKHARQRYSMPGTLNSRA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145978
ORF Size 1047 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145978.2
RefSeq Size 1514 bp
RefSeq ORF 1050 bp
Locus ID 213019
UniProt ID Q8R1G6
Cytogenetics 14 D2
MW 37.7 kDa
Summary Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145978" in other vectors (3)
SKU Description Size Price
MG205282 Pdlim2 (tGFP-tagged) - Mouse PDZ and LIM domain 2 (Pdlim2) 10 ug
$657.00
MR205282L3 Lenti ORF clone of Pdlim2 (Myc-DDK-tagged) - Mouse PDZ and LIM domain 2 (Pdlim2) 10 ug
$757.00
MR205282L4 Lenti ORF clone of Pdlim2 (mGFP-tagged) - Mouse PDZ and LIM domain 2 (Pdlim2) 10 ug
$757.00

Plasmid Map for MR205282

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Cloning scheme for MR205282

Circular map for MR205282

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